Amersham Pharmacia Biotech, Amersham place, England

Chemiluminescence’s reaction Kit

BstYI- restriction enzyme

Hind III- restriction enzyme

XbaI- restriction enzyme

Biogenes, Berlin, Germany

Anti-rabbit antibody

Bio-Rad, Munich

Tween 20

PBS

Diagnostic Chemicals Limited

isopropyl (-D)-thiogalactopyranoside

Invitrogen GmbH, Karlsruhe, Germany

BL21 (DE3) pLysS cells

Expression vector pRSETA

PCR-Kit

Merck, Darmstadt, Germany.

DNase

HCl

K₂HPo₄

KH₂po₄

MgSo₄

NaHCo₃

Na-K tartrat

Na₄P₂O₇

NaPo₄

Molecular Biochemicals Roche, Mannheim, Germany

Protease inhibitor mixture

proteinase K

Molecular Probes, Inc. USA

Secondary anti-mouse antibodies

Secondary anti-rabbit antibodies

Qiagen, Germany

NiNTA-agarose beads

Reanal-Budapest Hungary

Temed (N, N, N, N, teramethylethylene diamine)

Roth Karlsruhe

APS (ammonium peroxodisulfate)

EDTA

KCl

Methanol

NaCl

Na₂Co₃

Sodium lauryl sulfate (SDS)

Serva, Heidelberg, Germany

Acrylamid

Ampholyte mixture Servalyte

BSA (bovine serum albumin)

CaCl₂

Coomassie blue R-256

DTT (dithiothreitol)

Glucose

Glycerol

Glycine

Ponceau S

Sucrose

Sodium dodecylsulfate (SDS)

Tris

Sigma Chemical Co., St. Louis, MO

Albumin

β-mercaptoethanol

Bromophenol blue

Chloral hydrate

CuSo₄

Folin

Isopropanol

MgCl₂

Monoclonal antibody against α-actinin

NaN₃

Paraform aldehyde

Pepstatin A

RNAse A

Thiourea

Urea