Aguirre-Arteta, Ana Maria: REGULATION OF DNA METHYLATION DURING DEVELOPMENT: ALTERNATIVE ISOFORMS OF DNA METHYLTRANSFERASE

i

Abbreviations

 

A

A:

adenine

Ab:

antibody

A260:

absorbance at 260 nm

A280:

absorbance at 280 nm

Amp:

ampicillin

AMV:

avian myeloblastosis virus

AP:

alkaline phosphatase

APS:

ammonium persulphate

Arg:

arginine

Asp:

aspartate

ATP:

adenosine triphosphate

 

B

B:

BamHI

bp:

base pair

BSA:

bovine serum albumin

 

C

C:

cytosine

cDNA:

complementary DNA

COS-7 cells:

african green monkey kidney fibroblast-like cells transformed with SV40 T antigen

CpG:

cytosine-guanine doublet

cpm:

counts per minute

CTP:

cytosine triphosphate

Cys:

cysteine

 

D

D:

diplotene

Da:

dalton

dATP:

deoxyadenosine triphosphate

dCTP:

deoxycytidine triphosphate

ddNTP:

2‘, 3‘-dideoxynucleotide-5‘-triphosphate

dGTP:

deoxyguanosine triphosphate

DMPC:

dimethylpyrocarbonate

DMSO:

dimethyl sulfoxide


ii

dMTase:

DNA demethylase

DNA:

deoxyribonucleic acid

DNA MTase:

DNA methyltransferase

DNase:

deoxyribonuclease

dNTP:

2‘-deoxynucleotide-5‘- triphosphate

dpc:

day post coitum

DTT:

dithiothreitol

dTTP:

deoxythymidine triphosphate

 

E

E:

EcoRI

EDTA:

ethylenediaminetetraacetic acid

ES cells:

embryonic stem cells

 

F

F:

forward

FCS:

fetal calf serum

FITC:

fluorescein isothiocyanate

 

G

G:

guanine

GAPDH:

glyceraldehyde phosphate dehydrogenase

Gln:

glutamine

Glu:

glutamate

Gly:

glycine

g:

gram

GTP:

guanosine triphosphate

 

H

h:

hour

HDAC:

histone deacetylase

HhaI:

Haemophilus haemolyticus

Hoechst 33258:

2‘ (4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5‘ bi-1H-benzimidazole

HRP:

horseradishperoxidase

 

I

IgG:

immunoglobulin G

In:

intermediate spermatogonia


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IPTG:

isopropylthio-beta-D-galactoside

 

K

kb:

kilobase

kDa:

kilo Dalton

 

L

L:

leptotene

LB:

Luria Bertani

Lys:

lysine

 

M

M:

molar (mol/l)

mA:

milliampere

MB:

myoblast

5 mC:

C5-methylcytosine

MDG1:

microvascular endothelial differentiation gene 1

MeCP:

methyl-CpG-binding protein

mg:

milligram

min:

minute

ml:

milliliter

mmol:

millimol

MoMuLV:

moloney murine leukemia virus

MOPS:

3-(N-morpholino) propanesulfonic acid

mRNA:

messenger RNA

MT:

myotube

MTase:

methyltransferase

 

N

NF_B:

NF-kappa B

ng:

nanogram

NLS:

nuclear localization signal

nm:

nanometer

 

O

OD:

optical density

ORF:

open reading frame

 

P

P:

pachytene


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PAGE:

polyacrylamide electrophoresis

PBHD:

polybromo-1 protein homologous domain

PBS:

phosphate buffer saline

PCNA:

proliferating cell nuclear antigen

PCR:

polymerase chain reaction

PGC:

primordial germ cell

pH:

negative log of hydrogen ion concentration

Phe:

phenylalanine

Pl:

preleptotene

pRb:

retinoblastoma protein

Pro:

proline

PVDF:

polyvinylidenfluorid

pVHL:

von Hippel-Lindau tumor suppressor protein

Pwo:

Pyrococcus woesei

 

R

R:

reverse

RACE:

rapid amplification of cDNA ends

RFTS:

replication foci targeting sequence

RNA:

ribonucleic acid

RNase:

ribonuclease

rRNA:

ribosomal RNA

RT:

reverse transcription

 

S

S:

SalI

SAH/AdoHCy:

S-adenosyl-L-homocysteine

SAM/AdoMet:

S-adenosyl-L-methionine

SDS:

sodium dodecyl sulfate

Ser:

serine

SSC:

standard saline citrate

SV40:

simian virus 40

 

T

T:

thymine

TAE:

tris acetate EDTA

Taq:

Thermus aquaticus


v

TBS:

tris buffered saline

t-exon:

testis specific exon

TE:

tris EDTA

TEMED:

N, N, N‘, N‘-tetramethyl-ethylendiamine

Tf:

transcription factor

Tm:

melting temperature

TRD:

target recognition domain

Tris:

tris (hydroxymethyl)-aminomethane

tRNA:

transfer RNA

TTP:

thymidine triphosphate

Tyr:

tyrosine

 

U

U:

uridine

UTP:

uridine triphosphate

UV:

ultraviolet light

 

V

V:

volt

 

X

X:

XbaI

X-gal:

5-bromo-4-chloro-3-indolyl-?-D-galactopyranoside

 

Z

Z:

zygotene

Zn:

Zinc

 

µ

µCi:

microCurie

µg:

microgram

µl:

microliter


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