Molecular Epidemiology, Clinical Molecular Diagnosis and Genetic Diversity of Cutaneous Leishmaniasis in Jericho, Palestine Amer Al-Jawabreh111.121.2341.31.3.11.3.21.4561.5781.61.791.7.110563#296111.7.212583#2611.7.31.81313#51.9141.101.10.1151.10.216605#386171.10.318277#2201.10.419604#202201.10.5605#255211.10.6221.10.71.11231.12241.132526272829451#22022.12.1.13031656#3922.22.2.12.2.2322.2.32.2.4332.32.3.1342.3.22.435478#293362.52.5.1372.5.22.5.32.6382.72.7.12.7.1.1392.7.1.22.7.1.340412.7.22.82.8.142599#3542.943595#472.9.1442.9.2452.9.32.9.42.10462.10.1472.10.22.10.32.10.4482.10.52.112.1249502.12.12.12.22.12.3512.12.42.135214#175333.13.1.13.1.1.15354288#2063.1.1.23.1.1.3555657452#5123.1.258339#3425960565#25761499#5363.23.2.162604#8773.2.263605#8893.2.364605#9263.2.465574#395567#3553.33.3.166673.3.26869605#3463.3.370605#3173.414#1714#1771627#3343.53.5.1614#74972733.5.23.5.33.5.3.174465#23975605#851763.5.3.2574#186773.5.478624#840636#79479625#7563.5.5803.5.681458#311481824.14.1.1838485864.1.2878889904.2914.2.192934.39495964.4974.4.198994.4.21004.4.31014.5102List of AbbreviationsReferencesAcknowledgementsErklärungenTable of contentsHelp References Abrial, D., Calavas, D., Lauvergne, N., Morignat, E., and Ducrot, C. (2003). Descriptive spatial analysis of BSE in western France. Vet. Res.34 749-760 Abdeen, Z. A., Sawalha, S. S., Khanfar, H. M., Shtayeh, M. S., Warburg, A., Greenblatt, C. L., Jaffe, C. L, Baneth, G. (2002) Epidemiology of human and canine visceral leishmaniasis in the Jenin district, West Bank, 1989-1998. Am. J. Trop. Med. Hyg. 66(4) 329-33 Abdeen Z, Greenough G, Shaheen M, Tayback M. (2002) Nutritional Assessment of the West Bank and Gaza Strip http://www.carewbg.org Adler, S, & Theodor, O. (1957) Transmission of disease agents by phlebotomine sandflies. Ann Rev. Entomol.2 203-226. Al-Jawabreh, A., Schnur, L. F., Nasereddin, A., Schwenkenbecher, J. M, Abdeen, Z., Barghuthy, F., Khanfar, H. Presber, W and Schoenian, G. (2004). The recent emergence of Leishmania tropica in Jericho (A’riha) and its environs, a classical focus of Leishmania major. Trop. Med. Int. Hlth. 9 (7): 812–816 Al-Jawabreh, A., Barghuthy, F., Schnur, L. F., Jacobson, R. L., Schoenian, G. & Abdeen, Z. (2003) Risk assessment of cutaneous leishmaniasis in the endemic area of Jericho in Palestine. East. Med. Hlth. J.; 9 (4): 805-15. Al-Gindan Y, Kubba R, El-Hassan A, Omar, A. H., Kutty, M. K., and Saeed, M. B . (1989). Dissemination in cutaneous leishmaniasis. 3. Lymph node involvement. Int. J. Dermatol.; 28: 248 –54. Al-Majali O, Routh H, Abuloham O, Abuloham, O., Bhowmik, K. R., Muhsen, M., Hebeheba, H. (1997). A 2-year study of liquid nitrogen therapy in cutaneous leishmaniasis. Int. J. Dermatol.; 36: 460 –2. Al-Rai, K. (2005). Description of the natural status of child-bearing women and children under five among the Palestinian Bedouins in the Jordan Valley; MPH, School of Public Health, Al-Quds University, Jerusalem; 1-123. Alten, B., Caglar, S. S., Kaynas, S. and Simsek, F. M. (2003) Evaluation of protective efficacy of K-OTAB impregnated bednets for cutaneous leishmaniasis control in Southeast Anatolia-Turkey. J. Vec. Ecol.; 53-64. Anders, G., Eisenberger, C. L., Jonas, F., and Greenblatt, C. L. (2002). Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers. Trans. R. Soc. Trop. Med. Hyg.;96: 87-92 Andersen, K., Ibrahim, M. E., Theander, T. G., and Kharazmi, A. (1996). Random amplified polymorphic DNA for the differentiation of Leishmania donovani isolates from Sudan. Trans. R. Soc. Trop. Med. Hyg.;90, 204–205. Anis E, Leventhal A, Elkana Y, Wilamowski A & Pener H. (2001) Cutaneous leishmaniasis in Israel in the era of changing environment. Pub. Hlth. Rev.;29, 37–47. Arda, H. M. and Kamal, A. (1983). A report on cutaneous Leishmaniasis in the West Bank of Jordan. Jordan Med. J.; 17 (1): 57-63. Ashford, R.W., (1996). Leishmaniasis reservoirs and their significance in control. Clin. Dermatol;14: 523–32. Ashford, R. W. (1999). Cutaneous Leishmaniasis: Strategies for Prevention. Clin. Dermatol.; 17:327–332. Ashford, R. W. (2000). The leishmaniasis as emerging and remerging zonooses. Int. J. Parasitol.; 30: 1269-1281 Ashford R.W. & Sang D.K. (2001). Review of Leishmania tropica infection in East Africa. Proceedings of the Second International Congress on Leishmania and Leishmaniosis, May 20–24, 2001, Hersonissos, Crete, Greece, p. 14. Aucan, C., Walley, A. J., Hennig, B.J., Fitness, J., Frodsham, A., Zhang, L,, Kwiatkowski, D., Hill, A.V. (2003) Interferon-alpha receptor- 1 (IFNAR1) variants are associated with protection against cerebral malaria in the Gambia. Genes Immun.; 4: 275–82. Aviles, H., Belli, A., Armijos, A., Monroy, F. P., and Harris, E. (1999). PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J. Parasito; 85(2): 181-7. Ayala, F. J. (1998) Is sex better? Parasites say ‘‘no’’ Proc. Natl. Acad. Sci. USA;. 95: 3346–3348 Baneth, G., Dank, G., Keren-Kornblatt, E., Sekeles, E., Adini, I., Eisenberger, C. L., Schnur, L. F., King, R. and Jaffe, C. L. (1998). Emergence of visceral leishmaniasis in central Israel. Am. J. Trop. Med. Hyg.; 9: 722-725. Barker, D.C. (1989). Molecular approaches toDNAdiagnosis. Parasitology;99, 125–146. Behets, F., Kashamuka, M., Pappaioanou, M., Green, T. A., Ryder, R.W., Batter, V., George, J. R., Hannon, W. H., Quinn, T. C. (1992). Stability of human immunodeficiency virus type 1 antibodies in whole blood dried on filter paper and stored under various tropical conditions in Kinshasa, Zaire. J. clin. Microbiol.; 30: 1179-1182. Bell, G. I. & Jurka, J. (1997). The length distribution of perfect dimmer repetitive DNA is consistent with its evolution by an unbiased single-step mutation process. J. Mol. Evol.;44, 414–421 Belli, A., Rodriguez, B., Aviles, H., and Harris, E. (1998). Simplified polymerase chain reaction detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis. Am. J. Trop. Med. Hyg.; 58(1): 102-9. Ben-Ami, R., Schnur, L. F., Golan, Y., Jaffe, C. L., Mardia, T. and Zeltsera, D. (2002) Cutaneous Involvement in a Rare Case of Adult Visceral Leishmaniasis Acquired in Israel. J. Infection; 44: 181-184. Ben-Ismail, R., Garraoui, A., Chaded, M.K., Ben Salah, A., Abdouli, M., Zaafouri, B., Khadhraoui, B., Ftaiti, A., Sidhom, M. & Dellagi, K. (1997). Environmental changes to control Leishmania major cutaneous leishmaniasis in the epidemic focus of Sidi Bouzid (Tunisia). First World Congress on Leishmaniosis. Istanbul, May 5-9, 1997. Acta Parasitologica Turcica, 21: 138. Ben-Ismail, R., Ben Rachid, M.S., Gradoni, L., Gramiccia, M., Helal, H. & Bach-Hamba, D. (1987a) La Leishmaniose cutaneÂe zoonotique en Tunisie: eÂtude du reÂservoir dans le foyer de Douara. Annales de la SocieÂteÂ Belge de MeÂdecine Tropicale; 67: 335-343. Bethony, J., Silveira, A. M., Alves-Oliveira, L. F., Thakur, A., Gazzinelli, G., Correa-Oliveira, R., LoVerde, P. T. (1999). Familial resemblance in humoral immune response to defined and crude Schistosoma mansoni antigens in an endemic area in Brazil. J. Infect. Dis.; 180: 1665–73 Beverly, S. M., Ismach, R. B., and McMahon-Pratt, D. (1987). Evaluation of the genus Leishmania as revealed by comparison of nuclear DNA restriction fragment patterns. Proc. R. Soc. Lond. B. Biol. Sci.;84, 484–488. Bhattacharyya, R., R. Singh, T. K. Hazra, and Majumder, H. K. (1993). Application of polymerase chain reaction with specific and arbitrary primers to identification and differentiation of Leishmania parasites. FEMS Microbiol. Lett.; 114: 99–104. Blackwell, J.M. (1996). Genetic susceptibility to leishmanial infections: studies in mice and man. Parasitology, 112: 67-74 Booth, M. and Dunne, D.W. (2004). Spatial awareness in parasite immuno-epidemiology. Parasite. Immunol.; 26 (11-12): 499-507. Bowcock, A.M., Ruíz-Linares, A., Tomfohrde, J., Minch, E., Kidd, J.R., Cavalli-Sforza, L.L. (1994). High resolution human evolutionary trees with polymorphic microsatellites. Nature; 368: 455-457 Bray, R. S. (1987). Note on the history of cutaneous leishmaniasis in the Mediterranean and Middle East area. Parassitologia; 29 (2-3): 175-159. Brecelj, M., Pikelj, F., and Anderluh, A. (2000). Polymerase chain reaction as a diagnostic tool for detecting leishmania. Infection; 28: 111-113. Brohele, J., Ellegren, H. (1999). Microsatellite evolution: polarity of substitution within repeats and neutrality of flanking sequences. Proc. R. Soc. Lond. B. Biol. Sci.; 266: 825-833. Breniere, S. F., Telleria, J., Bosseno, M. F., Buitrago, R., Bastrenta, B., Guny, G., Banuls, A. L., Brewster, S. and Barjer, D. C. (1999). Polymerase chain reaction-based identification of New World Leishmania species complexes by specific kDNA probes. Acta Trop.;15 (73): 283-293. Bulle, B., Millon, L., Bart, L-M., Ga´llego, M., Gambarelli, F., Portu´s, M., Schnur, L., Jaffe, C. L. Fernandez-Barredo, S., Alunda, J. M. and Piarroux. R. (2002). Practical Approach for Typing Strains of Leishmania infantum by Microsatellite Analysis. J. Clin. Microbiol.;40 (9): 3391–3397. Campino, L., Cortes, S., Pires, R., Oskam, L. and Abranches, P. (2000). Detection of Leishmania in immunocompromised patients using peripheral blood spots on filter paper and the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis.; 19: 396-398. Cannan, T. (1945). Topographical studies in leishmaniasis in Palestine. J. Palestinian Arab Med. Ass.; 1, 4–12 Cavalli-Sforza, L.L., and Bodmer, W.F. (1971). The Genetics of Human Populations, p. 399, San Francisco, W.H. Freeman and Company. Chang, K. P. McGwire, B. S. (2002). Molecular determinants and regulation of Leishmania virulence. Kinetoplastid Biology and Disease; 1:1 Chakraborty, R. and Jin, L. (1993). A unified approach to study hypervariable polymorphisms: Statistical considerations of determining relatedness and population distances. In "DNA fingerprinting: State of the science. Pena, S.D.J., Chakraborty, R., Epplen, J.T., and Jeffreys, A.J. eds. Birkhauser Verlag Basel/Switzerland Coltman, D. W., Bowen, W. D., Wright, J. M. (1998) Birth weight and neonatal survival of harbour seal pups are positively correlated with genetic variation measured by microsatellites. Proc. R. Soc. Lond. B. Biol. Sci..; 265: 803–809 Constantine, C.C. (2003). Importance and pitfalls of molecular analysis to parasite epidemiology. Trends. Parasitol.; 19, 346–348 Cornuet, J.M. and Luikart, G. (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics;144: 2001-2014. Cooke, G.S., Aucan, C., Walley, A.J., Segal, S., Greenwood, B.M., Kwiatkowski, D.P., Hill, A,V. (2003). Association of Fcgamma receptor IIa (CD32) polymorphism with severe malaria in West Africa. Am. J. Trop. Med. Hyg.; 69: 565–568. Cooper, G., Amos W., Bellamy, Siddiqui, M. R., Frodsham, A., Hill, A. V. S. and David C. Rubinsztein, D. C. (1999) An empirical exploration of the (delta mu)2 genetic distance for 213 human microsatellite markers. Am. J. Hum. Genetics; 65: 1125-1133. Coulson, T. N., Pemberton, J. M., Albon, S. D., Beaumont, M., Marshall, T. C., Slate, J., Guiness, F. E. & Clutton-Brock, T. H. (1998). Microsatellites reveal heterosis in red deer. Proc. R. Soc. Lond. B.;265: 489- 495. Cox, F. E. G. (2002). History of Human Parasitology. Clin. Microbiol. Rev.; 595–612. Croft, S. L. and Yardley, V. (2002). Chemotherapy of Leishmaniasis. Cur. Pharmaceut. Des.;8, 319-342. Cutaneous Leishmaniasis in U.S. Military Personnel Southwest/Central Asia, 2002-2003. http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5242a1.htm D'Aignaux, J. H., Cousens, S. N., Delasnerie-Laupretre, N., Brandel, J.P., Salomon, D., Laplanche, J.L., Hauw, J. J. and Alperovitch, A. (2002). Analysis of the geographical distribution of sporadic Creutzfeldt-Jakob disease in France between 1992 and 1998. Int. J. Epidemiol.; 31(2): 495-496. De Beurmann, M. (1910). Leishmaniosis ulcerosa cutis un cas de bouton des pays chauds contracte a Jericho. Rev. Med. Hyg. Trop.; 7: 265–271. De Brujin, M. H., and D. C. Barker. (1992). Diagnosis of New World leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplastid DNA. Acta Trop.; 52: 45–58. De Brujin, M. H., L. A. Labrada, A. J. Smyth, C. Santrich, and D. C. Barker. (1993). A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis. Trop. Med. Parasitol.;44: 201–207. De Meeûs, T, and Balloux, F. (2004). Clonal reproduction and linkage disequilibrium in diploids: a simulation study. Infect. Genet. Evol.; 4 (4): 345-351. Desjeux, P. (1996). Leishmaniasis Public health aspects and control. Clin. Dermatol.; 14: 417–23. Desjeux, P. (1999). Global control and Leishmania HIV co-infection. Clin.Dermatol.; 17: 317–325. Desjeux, P. (2004). Leishmaniasis: current situation and new perspectives. Comp. Immunol. Microbiol. Infect. Dis.; 27 (5): 305-318 Dib, C., Faure, S., Fizames, C., Samson, D., Drouot, N., Vignal, A., Millasseau, P., Marc, S., Hazan, J., Seboun, E., Lathrop, M., Gyapay, G., Morissette,, J., Weissenbach, J. (1996). A comprehensive genetic map of the human genome based on 5,264 microsatellites. Nature.380: 152–154. Dieringer, D. & Schlotterer, C. (2003). Two distinct modes of microsatellite mutation processes: evidence from the complete genomic sequences of nine species. Genome Res.;13: 2242–2251. Dietrich, W. F., Miller, J ., Steen, R ., Merchant, M. A., Damron-Boles, D ., Husain, Z ., Dredge, R ., Daly, M. J., Ingalls, K. A ., O'Connor. (1996). . A comprehensive genetic map of the mouse genome Nature. 14 (380): 149-152. Dodd, D. M., and J. R. Powell. (1985). Founder-flush spe ciation: an update of experimental results with Drosophila . Evolution; 39: 1388–1392. Dowlati, Y. (1996). Cutaneous Leishmaniasis: Clinical Aspect. Clin. Dematol.; 14: 425431 Dubois, D., and Prade, H. (1980). Fuzzy Sets and Systems: Theory and Applications, p. 24, New York, Academic Press. Dujardin, J.C., Bañuls, A. L., Llanos-Cuentas, A., Alvarez, E., Dedonker, S., Jacquet, D., Le Ray, D., Arevalo, J., Tibayrenc, M. (1995). Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru. Acta Trop.; 59: 293–307. Eisenberger, C. L. & Jaffe, C. L. (1999). Leishmania: identification of Old World species using a permissively primed intergenic polymorphic-polymerase chain reaction. Exp Parasitol.; 91(1): 70-77. Erratum in: Exp. Parasitol.;(1999); 92 (2): 159. Ellegren, H. (2004). Microsatellites: simple sequences with complex evolution. Nat. Rev. Genet.; 5 (6): 435-445. El Tai, N. O., Osman, O. F., El Fari, M., Presber, W. and Schonian, G. (2000). Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans. R. Soc. Trop. Med. Hyg.; 94: 1-5. El Tai, N. O., El Fari, M., Mauricio, I., Miles, M. A., Oskam, L., El Safi, S. H., Presber, W. H., and Schoenian, G. (2001). Leishmania donovani : Intraspecific Polymorphisms of Sudanese Isolates Revealed by PCR-based Analyses and DNA Sequencing. Exp. Parasito.; 97: 35–44. Environmental profile for the West Bank. (1995). Jericho District. Applied Research Institute- Jerusalem; 2. Estoup, A., Garnery, L., Solignac, M., and Cornuet, J.-M. (1995b). Microsatellite variation in honey bee populations: Hierarchical genetic structure and test of the IAM and SMM. Genetics;140: 679-695. Evans, D. (Ed.). (1989). Handbook on Isolation, Characterization and Cryopreservation of Leishmania. WHO, Geneva. Evans, D., Lanham, S. M., Baldwin, C. L., and Peters, W. (1984). The isolation and isoenzyme characterization of Leishmania braziliensis sp. from patients with cutaneous leishmaniasis acquired in Belize. Trans. R. Soc. Trop. Med. Hyg.; 78: 35–42. Färnert, A., Arez, A. P., Correia, A. T., Björkman, A., Snounou, G. and do Rosario, V. (1999). Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg.; 93, 50-53. Farzadegan, H., Noori, K.H. & Ala, F. (1978). Detection of hepatitis-B surface antigen in blood and blood products dried on filter paper. Lancet, I: 362-363. Felsenstein, J. (2004). PHYLIP: Phylogeny Inference Package, Version 3.6. University of Washington, Seattle, WA.

Fissore, C., Delaunay, P., Ferrua, B., Rosenthal, E., Del Giudice, P., Aufeuvre, J-P., Le Fichoux, Y., and Marty, P. (2004). Convenience of Serum for Visceral Leishmaniasis Diagnosis by PCR. J. Clin. Microbiol.; 42: 5332-5333.

Galiana, A., Moya, A. and Ayala, F. J. (1993). Founder-flush speciation in Drosophila pseudoobscura: a large-scale experiment. Evolution; 47: 432–444. George, M., Wiklund, L., Aastrup, M., Pousette, J., Thunholm, B., Saldeen, T. Wernroth, L. B. Zarén B. and L. Holmberg, L. (2001). Incidence and geographical distribution of sudden infant death syndrome in relation to content of nitrate in drinking water and groundwater levels. European J. Clin. Invest.;31: 1083-1094. Gillis D, Klaus S, Schnur L, Piscopos, P., Maayan, S., Okon, E. and Engelhard, D. (1995). Diffusely disseminated cutaneous Leishmania major infection in a child with acquired immunodeficiency syndrome. Ped. Infect. Dis. J.; 14: 247–249. Goldstein, D. B., Linares, A. R., Cavalli-Sforza, L. L. and Feldman, M.W. (1995b). An evaluation of genetic distances for use with microsatellite loci. Genetics;139: 463-471. Goldstein, D. B. and Pollock, D. D. (1997). Launching microsatellites: a review of mutation processes and methods of phylogenetic inference. J. Hered.; 88: 335-342. Gordis, L. (1996). Epidemiology. Philadelphia: Saunders. Gottelli, D., Sillero-Zubiri, C., Applebaum, G. D., Roy, M. S., Girman, D. J. Garcia- Moreno, J., Ostranders, E. A., and Wayne, R. K. (1994). Molecular genetics of the most endangered canid: the Ethiopian wolf Canis simensis. Mol. Ecol.; 3: 301–312. Goudet, J. (1995). FSTAT (Version 1.2): A computer program to calculate F- statistics. J. Heredity; 86 (6): 485-486. Greenblatt, C.L., Schlein, Y. and Schnur, L.F. (1985). Leishmaniasis in Israel and vicinity. In: Chang, C., Bray, D. (Eds.). Leishmaniasis, Elsevier, London, pp. 415–26. Gunders, A. E., Lidor, R., Montilio, B. and Amitai, P. (1968). Isolation of Leishmania sp isolated from rodents in Israel. Nature, London, 219: 85-86. Gregorio, D.I., Kulldorff, M., Barry, L., Samocuik, H. and Zarfos, K. (2001). Geographical differences in primary therapy for early-stage breast cancer. Ann. Surg. Oncol.; 8 (10): 844-849.

Guthrie, R. & Susi, A. (1963). A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants.Pediatrics, 32:338-343.

Hancock, J. M. (1999). Microsatellites and other simple sequences: genomic context and mutational mechanisms. In: Microsatellites: Evolution and Applications (eds Goldstein DB, Schl.tterer C), pp. 1-9. Oxford University Press, Oxford. Harris, E., Kropp, G., Belli, A., Rodriguez, B., and Agabian; N. (1998). Single-Step Multiplex PCR Assay for Characterization of New World Leishmania Complexes. J. Clin. Microbiol.; 36 (7): 1989-1995. Harrison’s Principles of Internal Medicine, 12th ed., J.Wilson et al. (eds.), pp. 789–790, McGraw-Hill, New York, 1991. Copyright © 1991 McGraw-Hill, Inc. Hartl, D.L. and Clark, A.G. (1997). Principles of population genetics. 3rd edition. Sunderland (MA): Sinauer Associates. Hernandez-Montes, O., Monroy-Ostria, A., McCann, S. and Barker, D. C. (1998). Identification of Mexican Leishmania species by analysis of PCR amplified DNA. Acta Trop.; 71: 139-153. Henri, S., Chevillard, C., Mergani, A. Paris, P., Gaudart, J., Camilla, C., Dessein, H., Montero, F., Elwali ,N. E., Saeed, O.K., Magzoub, M., Dessein, A.J. (2002). Cytokine regulation of periportal fibrosis in humans infected with Schistosoma mansoni : IFN-gamma is associated with protection against fibrosis and TNF-alpha with aggravation of disease. J. Immunol.;169: 929–936. Herwaldt, B. L. (1999 a). Leishmaniasis. Lancet;354:1191-1199. Hoelzel, A. R . (1999), Impact of population bottlenecks on genetic variation and the importance of life-history; a case study of the northern elephant seal. Biol. J. Linnean Soc.;68: 23–39. Hoelzel, A. R., Natoli, A., Dahlheim, M. E., Olavarria, C., Baird, R. W. and Black, N. A. (2002). Low worldwide genetic diversity in the killer whale ( Orcinus orca ): implications for demographic history.Proc. R. Soc. Lond. B.; 269:1467–1473. Huntemüller. (1914). Neuartige Parasitenbefunde bei der Jerichobeule. Centralbl. F. Bakt., Abt. I. Orig. Bd.; 73: 137-141. International Human Genome Sequencing Consortium. (2001). Initial sequencing and analysis of the human genome. Nature. 409: 860–921. Jaber, K. (1987). The Leishmaniasis Reporting System in Jericho and West-Bank. MPH thesis. Hebrew University, Jerusalem. 1-54. Jackson, P. R., Wohlhieter, J. A., Jackson, J. E., Sayles, P., Diggs, C. L., and Hockmeyer, W. T. (1984). Restriction endonuclease analysis of Leishmania kinetoplast DNA characterizes parasites responsible for visceral and cutaneous diseases. Am. J. Trop. Med. Hyg.; 33: 808–819. Jacobson R.L., Eisenberger, C.L., Svobodová, M., Baneth, G., Sztern, J., Carvalho, J., Naseredeen, A., El Fari, M., Shalom, U., Volf, P., Votýpka, J., Dedet, J.-P., Pratlong, F., Schönian, G., Schnur, L.F., Jaffe, C.L. & Warburg, A. (2003). Outbreak of cutaneous leishmania-sis in Northern Israel. J. Infect. Dis.; 188: 1065–1073. Jacobson, R. L. (2003).Leishmania tropica (Kinetoplastida: Trypanosomatidae) – a perplexing parasite. Folia Parasitol.; 50: 241–250. Jaffe, C. L., Baneth, G., Abdeen, Z., Schlein, Y. and Warburg, A. (2004). Leishmaniasis in Israel and the Palestinian Authority. Trends Parasitol.;20: 328-332. Jaffe C.L. and Sarfstein R. (1987). Species-specific anti- bodies to Leishmania tropica (minor) recognize somatic antigens and exometabolites. J. Immunol.; 139: 1310– 1319. Jakupciak, J. P. and Wells, R. D. (2000). Gene conversion (recombination) mediates expansions of CTG.CAG repeats. J. Biol. Chem.; 275: 4003-4013. Jamjoom, M.B., Ashford, R.W., Bates, P.A., Kemp, S.J., Noyes, H.A., (2002a). Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex. Ann. Trop. Med. Parasitol.; 96: 265–270. Jamjoom, M.B., Ashford, R.W., Bates, P.A., Kemp, S.J., Noyes, H.A. (2002b). Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major. Mol. Ecol. Notes.; 2: 104–106. Jarne, P., and Lagoda, P. J. L. (1996). Microsatellites, from molecules to populations and back. Trends Ecol. Evol.; 11:424-429. Jawabreh, A., Nasereddin, A. and Schnur L.F. (2001). Leishmania tropica From The Jericho District Of The Palestinian Authority. In WorldLeish2 conference, Poster 49. Crete-Greece, 20-24.05.2001. Jiménez, M., Alvar, J., and Tibayrenc, M. (1997). Leishmania infantum is clonal in AIDS patients too: epidemiological implications. AIDS, 11: 569–573. Jones, T. C., W. D. Johnson, Jr., A. C. Barretto, E. Lago, R. Badaro, B. Cerf, S. G. Reed, E. M. Netto, M. S. Tada, T. F. Franca, Wiese, K., Gollgthtly, Fikrig, F., Costa, J. M. L, Cuba, C. C, Marsden, P. D. (1987). Epidemiology of American cutaneous leishmaniasis due to Leishmania braziliensis braziliensis. J. Infect. Dis.; 156: 73–83. Kamhawi, S., Modi, G.. B., Pimenta, P. F., Rowton, E. & Sacks, D. L. (2000). The vectorial competence of Phlebotomus sergenti is specific for Leishmania tropica and is controlled by species-specific, lipophosphoglycan-mediated midgut attachment. Parasitology; 121: 25–33. Katakura, K., Lubinga, C., Chitambo, H., and Tada, Y. (1997). Detection of Trypanosoma congolense and T. brucei subspecies in cattle in Zambia by polymerase chain reaction from blood collected on a filter paper. Parasitol. Res.; 83: 241- 245. Katakura, K., Kawazu, S-I., Naya, T., Nagakura, K., Ito, M., Aikawa, M., Qu, J-Q., Guan, L-R., Zuo, X-P., Chai, J-J., K.-P. Chang, and Matsumoto, Y. (1998).Diagnosis of Kala-Azar by Nested PCR Based on Amplification of the Leishmania Mini-Exon. Gene. J. Clin. Microbiol..; 36 (8): 2173-2177. Khamesipour, A., Dowlati, Y, Asilian, A., Hashemi-Fesharki, R., Javadi, J, Noazin, S. and Modabber, F. (2005). Leishmanization: Use of an old method for evaluation of candidate vaccines against leishmaniasis. Vaccine; 23: 3642–3648. Killick-Kendrick, R. (1999). The biology and control of Phlebotomine sand flies. Clin. Dermatol.; 7 (3): 279-289. Kidd, K. K., Morar, B., Castigione, C.M., Zhao, H., Pakstis, A.J., Speed, W.C., Bonne-Tamir, B., Lu, R.B., Goldman, D., Lee, C., Nam, Y. S., Grandy, D. K., Jenkins, T. and Kidd, J.R. (1998). A global survey of haplotype frequencies and linkage disequilibrium at the DRD2 locus. Hum. Genet.; 103: 211–227. Klassen, A. C., Kulldorff, M. and Curriero, F. (2005). Geographical clustering of prostate cancer grade and stage at diagnosis, before and after adjustment for risk factors. Int. J. Hlth. Geo.; 4 (1): 1. Klaucke, D. N., J. W. Buehler, S. B. Thacker, G. Parrish, F. L. Trowbridge, and R. L. Berkelman. (1988). Guidelines for evaluating surveillance systems. Mort. Morb. Wkly Rep.; 37 (5): 1. Klaus, S., Axelrod, O., Jonas, F., and Frankenburg, S. (1994). Changing patterns of cutaneous leishmaniasis in Israel and neighbouring territories. Trans. R. Soc. Tro.p Med. Hyg.;88:649–650. Klaus, S., and Frankenburg, S. (1999). Cutaneous Leishmaniasis in the Middle East, Clin. Dermatol.; 17: 137–141. Knapik, E. W, Goodman, A., Ekker, M., Chevrette, M., Delgado, J., Neuhauss, S., Shimoda, N., Driever, W., Fishman, M. C. and Jacob. H. J. (1998). A microsatellite genetic linkage map for zebrafish ( Danio rerio ). Nat. Genet., 18: 338-343. Kruglyak, L. (1999). Prospects for whole-genome linkage disequilibrium mapping of common disease genes. Nat. Genet.; 22: 139–144. Kubba R, El-Hassan A, Al-Gindan Y, Omer, A. H., Kutty, M. K. & Saeed, M.B. (1987). Dissemination in cutaneous leishmaniasis. I. Subcutaneous nodules. Int. J. Dermatol.; 26: 300–304. Kuhn, K. G. (1999). Global warming and leishmaniasis in Italy. Bull. Trop. Med. Int. Health., 7 (2): 1-2. Kulldorff, M. (1997). A spatial scan statistic. Communications in statistics. Theory Meth.; 26: 1481–1496. Kulldorff, M. and Nagarwalla N. (1995). Spatial disease clusters: detection and inference. Stat. Med.; 14: 799–810. Kulldorff, M., Rand, K., Gherman, G., Williams, G.. and De Francesco, D. (1998). SaTScan v2.1. Software for the spatial and space-time scan statistics. Bethesda, MD: National Cancer Institute. Kulldorff, M. (2001). Prospective time-periodic geographical diseases surveillance using a scan statistic. J. R. Stat. Soc.; 164: 61–72. Kulldorff, M., Heffernan, R., Hartmtan, J., Assuncano, R. and Mostashari, F. (2005). A space-Time Permutation Scan Statistic for Disease Outbreak Detection. PLoS Medicine/ http://www.plosmedicine.org Kunst, C.G., Zerylnick, C., Karickhoff, L., Eichler, E., Bullard, J., Chalifoux, M., Holden, J.J., Torroni, A., Nelson, D.L., and Warren, S.T. (1996). FMR1 in global populations. Am. J. Hum. Genet.; 58: 513–522. Lachaud, L., Marchergui-Hammami, S., Chabbert, E., Dereure, J., Dedet, JP and Bastien, P. (2002). Comparison of Six PCR Methods Using Peripheral Blood for Detection of Canine Visceral Leishmaniasis. J. Clin. Microbiol.;40: 1210–1215. Lainson, R. & Shaw, J. J. (1986). Leishmania: Taxonomie et Phylogenese: Applications Eco-Epidemiologiques. (Coll. Int. CNRS/INSERM, Paris), pp. 241-245. Langmuir, A. D. (1963). The surveillance of communicable diseases of national importance. N. Eng. J. Med.;268: 182–192. Lanotte, G., Rioux, J. A. & Serres, R. (1986). Leishmania: Taxonomie et Phylogenese: Applications Eco-Epidemiologiques. (Coll. Int. CNRS/INSERM, Paris), pp. 27-40. Laskay, T., Miko, T. L., Negesse, Y., Solbach, W., Rollinghoff, M. and Frommel, D. (1995). Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg. ; 89 (3): 273-275. Last, J. M. (2001). A dictionary of epidemiology, 4th edn, Oxford: Oxford University Press. Lawyer, P. G., Mebrahtu, Y.B., Ngumbi, P.M., Mwanyumba, P., Mbugua, J., Kiilu, G., Kipkoech, D., Nzovu, J., Anjili, C.O. (1991) Phlebotomus guggisbergi (Diptera: Psychodidae), a vector of Leishmania tropica in Kenya. Am. J. Trop. Med. Hyg.; 44: 290– 298. Le-Blancq, S. M. & peters, W. (1986). Leishmania in the Old World: II. Hydrogenity among L.tropica zymodemes. Trans. R. Soc. Trop. Med. Hyg.; 80: 113-119. Le-Blancq, S. M., Schnur, L. F. and Peters, W. (1986). Leishmania in the Old World: I. The geographical and hostal distribution of L. major zymodems. Trans. R. Soc. Trop. Med. Hyg.; 80: 99-112. Lemrani, M. and Nejjar, R. (2002). A new Leishmania tropica zymodeme —causative agent of canine visceral leishmaniasis in northern Morocco.Ann. Trop. Med. Parasitol.;96(6): 637–638. Levinson, G. & Gutman, G. A. (1987). High frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 in Escherichia coli K-12. Nucleic Acids Res. 15: 5323–5338. Levy, S. and Jennings, E. R. (1950). The use of control charts in the clinical laboratory. Am. J. Clin. Pathol.; 20: 1059-1066. Lewis, P. O., and Zaykin, D. (2001). Genetic Data Analysis: Computer program for the analysis of allelic data. Version 1.0 (d16c). Free program distributed by the authors over the internet from http://lewis.eeb.uconn.edu/lewishome/software.html Lighthall, G. K., and Giannini, S. H. (1992). The chromosomes of Leishmania. Parasitol. Today. 8: 192–199. Long, A.D., and Langley, C.H. (1999). The power of association studies to detect the contribution of candidate genetic loci to variation in complex traits. Genome Res.; 9: 720–731. Lopez, M., R. Inga, M. Cangalaya, J. Echevarria, A. Llanos Cuentas, C. Orrego, and J. Arevalo. (1993). Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work. Am. J. Trop. Med. Hyg.; 49: 348–356. Macedo, A. M., Melo, M. N., Gomes, R. F., and Pena, S. D. J. (1992). DNA fingerprints: A tool for identification and determination of the relationships between species and strains of Leishmania. Mol. Biochem. Parasitol.; 53: 63–70. Mathis, A. & Deplazes, P. (1995). PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from humans and dogs. J. Clin.l Microbiol.;33:1145-1149. Matsumoto, T., Hashiguchi, Y., Gomez, E.A., Calvopiña, M.H., Nonaka, S., Saya, H. and Mimori, T. (1999). Comparison of PCR results using scrapejexudate, syringe-sucked luid and biopsy samples for diagnosis of cutaneous leish- maniasis in Ecuador. Trans. R. Soco. Trop. Med. Hyg.; 93: 606-607. Maynard Smith, J. (1999). The Detection and Measurement of Recombination from Sequence Data. Genetics,153: 1021-1027. Meffert, L. M., and Bryant, E. H. (1991). Mating propensity and courtship behavior in serially bottlenecked lines of the housefly. Evolution; 45: 293–306. Meredith, S. E., Zijlstra, E. E., Schoone, G. J., Kroon, C. C., Van Eys, G. J. Schaeffer, K. U., el-Hassan, A. M. and Lawyer, P. G., L. (1993). Develeopment and application of the polymerase chain reaction for the detection and identification of leishmania parasites in clinical material. Arch. Inst. Pasteur Tunis. 70: 419-431. Michalakis, Y. and Excoffier, L. (1996). A generic estimation of population subdivision using distances between alleles with special reference for microsatellite loci. Genetics.142: 1061-1064. Miles, M. A., Povoa, M. M., Sousa, A. A., Lainson, R. L., and Shaw, J. J. (1980). Some methods for the enzymatic characterization of Latin American Leishmania with particular reference to Leishmania mexicana amazonensis and subspecies of Leishmania hertigii. Trans. R. Soc. Tro.p Med. Hyg; 74: 244–253. Mimori, T., Sasaki, J., Nakata, M., Gomez, E.A., Uezato, H., Nonaka, S., Hashiguchi, Y., Furuya, M. and Saya, Y., (1998). Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene;210:179-186. Mimori, T., Matsumoto, T., Calvopiña, M.H., Gomez, E.A. Saya, H., Katakura, K., Nonaka, S., Shamsuzzaman, S.M., Hashiguchi, Y. (2002). Usefulness of sampling with cotton swab for PCR-diagnosis of cutaneous leishmaniasis in the New World. Acta Trop.;81: 197-202. Minch, E., Ruiz-Linares, A., Goldstein, D., Feldman, M., and Cavalli-Sforza, L.L. (1997). Microsat (version 1.5): A computer program for calculating various statistics on microsatellite allele data [Online]. Available at http://lotka.stanford.edu/microsat (verified 25 May 1999). Ministry of Health-PHIC. (2004). Health indicators in Palestine 2003, http://www.moh.gov.ps Momeni, A. & Aminjavaheri, M. (1995). Treatment of recurrent cutaneous leishmaniasis. Int. J. Dermatol.; 34: 129 –33. Momeni AZ, Yotsumoto S, Mehregan DR, Mehregan AH, Mehregan DA, Aminjavaheri M, Fujiwara H, Tada J. (1996). Chronic lupoid leishmaniasis. Evaluation by polymerase chain reaction. Arch. Dermatol.;132 (2): 198-202 Morsy, A. T. (1996). Cutaneous leishmaniasisin Egypt (Review and comment). J. Egypt. Soc. parasitol.; 26 (1) Morton, N.E., and Collins, A. (1998). Tests and estimates of allelic association in complex inheritance. Proc. Natl. Acad. Sci.; 95: 11389–11393. Mostashari, F., Kulldorff, M., Hartman, J. J., James R. Miller, J. R., and Kulasekera, V. (2003). Dead Bird Clusters as an Early Warning System for West Nile Virus Activity. Emerg. Infec. Dis.; 9 (6): 641-646. Motazedian, H., Karamian, M., Noyes, H. A. and Ardehali, S. (2002). DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCRAnn. Trop. Med. Parasitol.;96 (1): 31–34. Mountain, J. L. and Cavalli-Sforza, L. L. (1997). Multilocus genotypes, a tree of individuals, and human evolutionary history. Am. J. Hum. Genet.; 61: 705-718. Murray, B. W. (1996). The Estimation of Genetic Distance and Population Substructure from Microsatellite allele frequency data. http://helix.biology.mcmaster.ca/brent/brent.html Musso, O., Sommer, P., Drouet, E., Cotte, L., Neyra, M., Grimaud, J., and Chevallier, M. (1996). In situ detection of human cytomegalovirus DNA in Gastrointistinal biobsies from AIDS patients by means of various PCR-derived methods. J. Vir. Meth.; 56:125-137 . Naggan, L., Gunders, A. E., Dizian, R., Dannon,Y., Shibolet, S., Ronen, A., Schneeweiss, R. & Michaeli, D. (1970). Ecology and attempted control of cutaneous Leishmaniasis arround Jericho in the Jordan Valley. J. Infec. Dis.;427-431. Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 538-590. Nei, M. (1987). Molecular Evolutionary Genetics. Columbia University Press, New York, NY. Neoumine N. I. (1996). Leishmaniasis in the Eastern Mediterranean Region. East. Med. Hlth. J.; 2 (1): 94-101. Nielsen, C.M.; Bygbjeg, I.C. & Vestergaard, B.F. (1987). Detection of HIV antigens in eluates from whole blood collected on filterpaper. Lancet, I: 566-567. Nimri, L., Soubani, R. & Gramiccia, M. (2002). Leishmania species and zymodemes isolated from endemic areas of cutaneous leishmaniasis in Jordan. Kinetoplastid Biology and Disease 1:7 [Cited 2003 August 5]. Available from: http://www.kinetoplastids.com/content/1/1/7. NIST/SEMATECH. e-Handbook of Statistical Methods.http://www.itl.nist.gov/div898/handbook/ Noyes, H. A., Belli, A. A., and Maingon, R. (1996). Appraisal of various random amplified polymorphic DNA-polymerase chain reaction primers for Leishmania identification. Am. J. Trop. Med. Hyg.; 55: 98–105. O’Brien S. J.; Wildt, D. E.; Bush, M.; Caro, T. M.; Fitzgibbon, C.; Aggundey, I.; Leakey, R. E. (1987). East African cheetahs: evidence for two population bottlenecks. Proc. Natl. Acad. Sci.; USA. 84: 508–511. Ohta, T. & Kimura, M. (1973). A model of mutation appropriate to estimate the number of electrophoretically detectable alleles in a finite population. Genet. Res. 22:201–204. Osman, O. F., Oskam, L., Zijlstra, E. E., Kroon, N. C., Schoone, G. J., Khalil, E. T., El-Hassan, A. M. and Kager, P. A. (1997).Evaluation of PCR for diagnosis of visceral leishmaniasis. J. Clin. Microbiol.; 35 (10): 2454-2457. Osman, O. F, Oskam, L., Kroon, N. C., Schoone, G. J., Khalil, E. T., El Hassan, A. M., Zijlstra, E. E. and Kager, P. A. (1998). Use of PCR for diagnosis of post kala-azar dermal leishmaniasis (PKDL). J. Clin. Microbiol.;36 (6): 1621-1624. Oumeish, O. Y. (1999). Cutaneous Leishmaniasis: A Historical Perspective. Clin. Dermatol.; 17:249–254. Paetkau, D., Calvert, W., Stirling, I., Strobeck, C. (1995). Microsatellite analysis of population structure in Canadian polar bears. Mol. Ecol.;4: 347–354. Palestinian Central Bureau of Statistics (PCBS). (2005). Population Projections in the Palestinian Territory, Revised Series. Ramallah-Palestine. Panton, L.J., Tesh, R. B., Nadeau, K.C., Beverley, S.M.(1991). A test for genetic exchange in mixed infections of Leishmania major in the sandfly Phlebotomus papatasi.J. Protozool.; 38: 224–228. Peckova, J., Svobodova, M. and Volf, P. (2005). Midgut glycoproteins of sand flies and their role in vector-parasite interaction. In: WorldLeish3 conference, Palermo- Italy, 10-16.04.2005. p 190. Piarroux, P., Gambarelli, F., Dumon, H., Fontes, M., Dunan, S., Mary, C., Toga, B. and Quilici, M. (1994). Comparison of PCR with direct examination of bone marrow aspiration, myeloculture, and serology for diagnosis of visceral Leishmaniasis in immunocompromised patients. J. Clin. Microbiol.; 32(3): 746-749. Pirmez, C., da Silva Trajano, V., Paes-Oliveira Neto, M. Maria da-Cruz, A., Gonçalves-da-Costa, S. C., Catanho, M., Degrave, W., and Fernandes, O. (1999). Use of PCR in diagnosis of human American tegumentary leishmaniasis in Rio de Janeiro, Brazil. J. Clin. Microbiol..; 37: 1819–1823. Pritchard, J. K. Stephens, M., and Donnelly, P. (2000a). Inference of population structure using multilocus genotype data. Genetics; 155: 945-959. Pogue, G. P., Koul, S., Lee, N. S., Dwyer, D. M., and Nakhasi, H. L. (1995a). Identification of intra and interspecific Leishmania genetics polymorphisms by arbitrary primed polymerase chain reaction and use of polymorphic DNA to identify differentially regulated genes, Parasitol Res.; 81: 282–290. Pogue, G. P., Lee, N. S., Koul, S., Dwyer, D. M., and Nakhasi, H. L. (1995b). Identification of differentially expressed Leishmania donovani genes using arbitrarily primed polymerase chain reaction. Gene; 165: 31–38. Queller; D. C., Strassmann, J. E., Hughes, C.R. (1993). Microsatellites and kinship. Trends Ecol. Evol.;8: 285–288. Quesenberry, C. P. (1993). The effect of sample size on estimated limits for X (with hyperdash) and X control charts, J. Quality Tech.;25(4): 237-247. Rajabi, P., Jalayer T., Asilian, A. and Abbas-Zadeh, M. R. (1999). Fine needle aspiration cytology compared with scraping method for microscopic diagnosis of cutaneous leishmaniasis. Res. Med. (Pajouhesh dar Pezeshki); 4 (1): 3-6. Ramirez, J. R., Agudelo, S., Muskus, C,. Alzate, J. F., Berbrich, C, Barker, D, and Velez, I. D. (2000). Diagnosis of Cutaneous Leishmaniasis in Colombia: the Sampling Site within Lesions Influences the Sensitivity of Parasitologic Diagnosis. J. Clin. Microbiol..; 38 (10): 3768-3773. Reed, T. E. (1973). Number of gene loci required for accurate estimation of ancestral population proportions in individual human hybrids. Nature. 244: 575–576. Reed, S. G. (1996). Diagnosis of Leishmaniasis. Clin. Dermatol.; 24: 471-478. Reithinger, R., Davies, C. R., (2002). American cutaneous leishmaniasis in domestic dogs: an example of the use of the polymerase chain reaction for mass screening in epidemiological studies. Trans. R. Soc. Trop. Med. Hyg., 1: 123-126. Reithinger, R., Espinoza, J. C., Courtenay, O. and Clive R. Davies, C. R. (2003a). Evaluation of PCR as a Diagnostic Mass-Screening Tool to Detect Leishmania (Viannia) spp . in Domestic Dogs (Canis familiaris). J. Clin. Microbiol.;41: 1486–1493. Reithinger, R., Mohsen, M., Aadil, K., Sidiqi, M., Erasmus, P., and Coleman, P. G (2003b). Anthroponotic Cutaneous Leishmaniasis, Kabul, Afghanistan. Emerg. Infec. Dis.; 9 (6): 727-729. Reithinger, R., Mohsen, M., Wahid, M., Bismullah, M., Quinnell, R. J., Davies, C. R., Kolaczinski, J. and David, J. R. (2005). Efficacy of thermotherapy to treat cutaneous leishmaniasis caused by Leishmania tropica in Kabul, Afghanistan: a randomized, controlled trial. Clin. Infect. Dis.;40 (8): 1148-1155. Richard, G.F., Paques, F. (2000). Mini- and microsatellite expansions the recombination connection. EMBO Rep.;1: 122-126. Ringo, J., Rockwell, R. and Dowse, H. (1985). An experiment testing two hypotheses of speciation. Am. Natu ralist.;126: 642–661. Rioux, J. A., Lanotte, G., Pratlong, F., Martini, A., Serres, E. & Belmonte, A. (1986). Centre international de cryoconservation, d’identi. Cation enzymatique et d’etude taxonomique des Leishmania (Montpellier, France). In Leishmania: Taxonomy and Phylogeny, ed. Rioux, J. A. pp. 485–502. Montpellier: Institut Mediterranean d’Etudes Epidemiologique et Ecologiques Rioux, J.-A., Petter, F., Zahaf, A., Lanotte, G., Houin, R., Jarry, D., Périéres, J., Martini, A. & Sarhani, S. (1986c). Isolement de Leishmania major Yakimoff et Schokhor, 1914 (Kinetoplastida-Trypanosomatidae) chez Meriones shawi (Duvernoy, 1842) (Rodentia-Gerbillidae) en Tunisie. Annales de Parasitologie Humaine et CompareÂe, 61, 139-145. Rioux, J. A., Lanotte, G., Serres, E., Pratlong, F., Bastien, P. and Perieres, J. (1990). Taxonomy of Leishmania. Use of enzymes. Suggestions for a new classification. Ann. Parasitol. Hum. Comp.; 65:111–125. Robinson, R. J., Agudelo, S., Muskus, C., Alzate, J. F., Berberich, C., Barker, D. C., Velez, I. D. (2002). The method used to sample ulcers influences the diagnosis of cutaneous leishmaniasis.Trans. R. Soc. Trop. Med. Hyg.; 96 (1): 169-171. Rodrigues, E. H. R., de Brito, M. E. F., Mendonca, M. G., Werkhäuser, R. P. Coutinho, E. M., Souza, W. V., de Albuquerque, M. F. P. M., Jardim, M. L. and Abath, F. G. C. (2002). Evaluation of PCR of American Cutaneous Leishmaniasis in an Area of Endemicity in Northeastern Brazil. J. Clin. Microbiol.; 40 (10): 3572-3576. Rodriguez, N., Guzman, B., Rodas, A., Takiff, H., Bloom, B. R. and Jacinto Convit, J. (1994). Diagnosis of Cutaneous Leishmaniasis and Species Discrimination of Parasites by PCR and Hybridization. J. Clin. Microbiol.; 32 (9): 2246-2252. Rodrigues, V. Jr, Abel, L., Piper, K. & Dessein, A. J. (1996). Segregation analysis indicates a major gene in the control of interleukine-5 production in humans infected with Schistosoma mansoni. Am. J. Hum. Genet.; 59: 453–461. Romero, G. A., Guerra, M.V., Paes, M.G., Cupolillo, E., Bentin Toaldo, C., Macedo, V. O., Fernandes, O. (2001). Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis. Acta Trop.; 79(3): 225-229. Romualdi, C., Balding, D., Nasidze, I. S., Risch, G., Robichaux, M., Sherry, S. T., Stoneking, M., Batzer, M. A., and Barbujan, G. (2002). Patterns of Human Diversity, within and among Continents, Inferred from Biallelic DNA Polymorphisms. Genome Res.; 12 (4): 602-612. Rose, K., Curtis, J., Baldwin, T., Mathis, A., Kumar, B., Sakthianandeswaren, A., Spurck, T, Low Choy, J and Handman, E. (2004). Cutaneous Leishmaniasis in red kangaroos: isolation and characterization of the causative organism. Int. J. Parasitol.; 34: 655-664. Rosenberg, N. A., Pritchard, J. K., James, L., Weber, L., Cann, H. M., Kidd, K. K., Zhivotovsky, L. A., Feldman, M. W. (2002). Genetic Structure of Human Populations. Science; 298: 2381-2385. Rosenberg, N. A., Burke, T., Elo, K., Feldman, M. W., Freidlin, P. J., Groenen, M. A. M., Hillel, J., Ma¨ki-Tanila, A., Tixier-Boichard, M., Vignal, A. Wimmers, A., Klaus. A. and Weigend, S. (2001). Empirical Evaluation of Genetic Clustering Methods Using Multilocus Genotypes From 20 Chicken Breeds. Genetics; 159: 699–713. Rousset, F. (1996). Equilibrium values of measures of population subdivision for stepwise mutation processes. Genetics; 142: 1357–1362. Rousset, F. and Raymond, M, (1995). Testing heterozygote excess and deficiency. Genetics. 140: 1413-1419. Rossi, V., Wincker, P., Ravel, C., Blaineau, C., Pages, M., and Bastien P. (1994). Structural organisation of microsatellite families in the Leishmania genome and polymorphisms at two (CA)n loci. Mol. Biochem. Parasitol.; 65(2): 271-282. Ruby, R. H. (1995). Jericho: Dreams, Ruins, Phantoms. New York: Henry Holt, (350 p.) Russell, R., Iribar, M. P., Lambson, B., Brewster, S., Blackwell, J. M., Dye, C. & Ajioka, J. W. (1999). Intra and inter-species microsatellite variation in the Leishmania subgenus Viannia. Mol. Biochem. Parasitol.; 103: 71-77. Sack, U., Burkhardt, U., Borte, M., Schadlich, H., Berg, K. & Emmrich, F. (1998). Age-dependent levels of select immunological mediators in sera of healthy children. Clin. Diagn. Lab. Immunol;5: 28–32. Sacks, D. L., Kenny, R. T., Kreutzer, R. D., Jaffe, C. L., Gupta, A. K., Sharma, M. C., Sinha, S. P., Neva, F. A. and Saran, R. (1995). Indian kala-azar caused by Leishmania tropica. The Lancet, 345. Safaei, A., Motazedian, M. H., Vasei, M. (2002). Polymerase chain reaction for diagnosis of cutaneous leishmaniasis in histologically positive, suspicious and negative skin biopsies. Dermatology; 205 (1): 18-24. Saiki, R.K., Gelfand, D.H., Stoffel, S., Scharf, S.J., Higuchi, R., Hom, G.T., Mullis, K.B. and Erlich, H.A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science;239: 487-491. Saliba, E. K. and Oumeish, Y. O. (1999). Reservoir Hosts of Cutaneous Leishmaniasis. Clin. Dermatol.; 17: 275–277. Sambrook, J., Russell, R. W. (2001). Molecular cloning: A laboratory manual, 3rd ed. Cold Spring, NY: Cold Spring Harbor Laboratory Press. Satti, M. B., El-Hassan, A. M., Al-Gindan, Y., Osman, M. A., and Al-Sohaibani, M. O. (1989). Peripheral neural involvement in cutaneous leishmaniasis. A pathologic study of human and experimental animal lesions. Int. J. Dermatol.; 28(4): 243-247. Schallig, H. D., Oskam, L.(2002). Review: Molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification.Trop. Med. Int. Health.; 7 (8): 641-651. Schaid, D.J. (1996). General score tests for associations of genetic markers with disease using cases and their parents. Genet. Epidemiol.;. 13: 423–449. Schlein, Y., Warburg, A., Schnur, L. F., Gunders, A.E. (1982). Leishmaniasis in the Jordan Valley II. Sandflies and transmission in the central endemic area. Trans. R. Soc. Trop. Med. Hyg.; 76(5): 582-586. Schlein, Y., Warburg, A., Schnur, L.F., Le Blancq, S.M., Gunders, A.E., (1984). Leishmaniasis in Israel: reservoir hosts, sand fly vectors and leishmanial strains in the Negev, Arava and along the Dead-Sea. Trans. R. Soc. Trop. Med. Hyg. 78: 480–484. Schlein, Y., Jacobson, R. L., and Mueller, G. C. (2001). Sand Fly Feeding on Noxious Plants: A Potential Method For The Control of Leishmaniasis. Am. J. Trop. Med. Hyg.;65 (4): 300–303. Schloetterer, C. and Tautz, D. (1992). Slippage synthesis of simple sequence DNA. Nuc Acids Res.;20:211-215. Schonian, G.; Schweynoch, C.; Zlateva, K.; Oskam, L.; Kroon, N.; Gräser, Y. & Presber, W. (1996). Identification and determination of the relationship of species and strains within the genus Leishmania using single primers in the polymerase chain reaction. Mol. Biochem. Parasitol.; 77:19-29. Schonian, G., El Fari, M., Lewin, S., Schweynoch, C. and Presber, W. (2001). Molecular epidemiology and population genetics in Leishmania. Med. Microbiol. Immunol.;190: 61-63. Schonian, G., Nasereddin, A., Dinse, N., Schweynoch, C., Schallig, H. D., Presber, W. and Jaffe, C. L. (2003). diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn. Microbiol. Infect. Dis.; 47(1): 349-58. Schnur, L. F., Nasereddin, A., Eisenberger, C. L., Jaffe, C.L, El Fari, M., K., Anders, G., Killick-Kendrick, M., Killick-Kendrick, R., Dedet, J-P., Pratlong, F., Kanaan, M., Grossman, T., Jacobson, R. L., Schoenian, G. and Warburg, A. (2004). Multifarious Characterization Of Leishmania tropica From A Judean Desert Focus, Exposing Intraspecific Diversity And Incriminating Phlebotomus Sergenti As Its Vector. Am. J. Trop. Med. Hyg., 70 (4): 364-372. Schnur, L. F. & Jacobson, R. L. (1987). Parasitological Techniques. In: The leishmaniases in biology and medicine (Vol. 1). Peters, W, Killick-Kendrick, B (eds.). Academic press, London, U. K., 500- 539. Schnur, L. F., Sarfstein, R., Jaffe, C. L., (1990). Monoclonal antibodies against leishmanial membranes react with specific excreted factors (EF). Ann. Trop. Med. Parasitol.; 84: 447-456. Schnur, L. F., Zuckerman, A., Greenblatt, C. L. (1972). Leishmanial serotypes as distinguished by the gel diffusion of factors excreted in vitro and in vivo. Israel. J. Med. Sci.;8: 932-942. Schnur, L. F., Zuckerman, A. (1977). Leishmanial excreted factor (EF) serotypes in Sudan, Kenya and Ethiopia. Ann. Trop. Med. Parasitol.; 71: 273-294. Schuler, G. D, Boguski, M. S., Stewart, E. A. et al. (1996). A gene map of the human genome. Science; 274:540-546. Schuster, F. L., & Sullivan, J. J. (2002). Cultivation of clinically significant hemoflagellates. Clin. Microbiol. Rev.;15: 374-389. Schwenkenbecher, J. M., Frohlich, C., Gehre, F., Schnur, L. F and Schonian, G. (2004). Evolution and conservaation of microsatellite markers for Leishmania tropica . Infect. Gent. Evol.; 4: 99-105. Sharquie, K. E., Hassan, A. S., Hassan, S. A., Al-Hamamil, A. (2002). Evaluation of diagnosis of cutaneous leishmaniasis by direct smear, culture and histopathology. Saudi. Med. J.; 23: 925-928. Sheehan, T. J., DeChello, L. M, Kulldorff, M., Gregorio, D. I., Gershman, S. and Mroszczyk, M. (2004). The geographic distribution of breast cancer incidence in Massachusetts 1988 to 1997, adjusted for covariates.Int. J. Hlth. Geo.;3: 17. Shewhart, W. A., (1931). Economic Control of Quality of the Manufactured Product, Van Nostrand, New York, NY. Shiddo, S. A., A. Aden Mohamed, H. O. Akuffo, K. A. Mohamud, A. A. Herzi, H. Herzi Mohamed, G. Huldt, L. A. Nilsson, O. Ouchterlony, and R. Thorstensson. (1995). Visceral leishmaniasis in Somalia: prevalence of markers of infection and disease manifestations in a village in an endemic area. Trans. R. Soc. Trop. Med. Hyg.; 89: 361–365. Shinde, D., Lai, Y., Sun, F. & Arnheim, N, (2003). N . Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites. Nucleic Acids Res.; 31:974–980. Singh, S., Sivakumar, R. (2003). Recent Advances in the Diagnosis of Leishmaniasis. J. Postgrad. Med.; Online full text at http://www.jpgmonline.com Slatkin, M. (1995). A measure of population subdivision based on microsatellite allele frequencies. Genetics, 139: 457-462. Shriver, M. D., Jin, L., Boerwinkle, E., Deka, R., Ferrell, R.E., and Chakraborty, R. (1995). A novel me asure of genetic distance for highly polymorphic tandem repeat loci. Mol. Biol. Evol., 12(5): 914-920. Shriver, M. D., Robert, L. J., Ferrell, E. and Deka, R. (1997). Microsatellite Data Support an Early Population Expansion in Africa . Genome Res.; 7(6): 586-591. Souto, C. P., and Premoli, A.C. (2003). Genetic divergence among natural populations of Alstroemeria aurea D. Don: A dominant clonal herb of the under story in subalpine Nothofagus forests. Bot. Bull. Acad. Sin.; 44: 329-336. Stirnadel, H. A., Beck, H. P., Alpers, M. P., & Smith, T. A. (2000). Genetic analysis of IgG subclass responses against RESA and MSP2 of Plasmodium falciparum in adults in Papua New Guinea. Epidemiol. Infect.; 124: 153–162. Strauss-Ayali, D., Jaffe, C.L., Burshtain, O., Gonen, L. and Baneth, G., (2004). Polymerase chain reaction using noninvasively obtained samples, for the detection of Leishmania infantum DNA in dogs. J. Infect. Dis.; 189: 1729-1733. Strand, M., Prolla, T. A., Liskay, R. M. & Petes, T. D. (1993). Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. Nature; 365:274–276. Strelkova, M. V., Pacheco, R. S., Bulat, S. A. and Rakitskaya, T. A. (1997). Comparison of Some Molecular-genetic Techniques fo r Identification of Leishmania Circulating in Natural Foci of Zoonotic Cutaneous Leishmaniasis in the Central Asia Region.Mem. Inst. Oswaldo Cruz; 92 (1): 109-114. Sundar, S. and Rai, M. (2002). Laboratory Diagnosis of Visceral Leishmaniasis. Clin. Diag. Lab. Immunol.; 9 (5): 951–958. Swofford, D. L. (2001). PAUP* Phylogenetic Analysis Using Parsimony and other methods V. 4.0 beta. Sinauer Associates, Sunderland, MA. Takezaki, N. and Nei, M. (1996). Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA. Genetics; 144: 389–399. Tazi, L., Kreiswirth, B., Carrière, C. and Tibayrenc, M. (2002). Molecular epidemiology of Mycobacterium tuberculosis and its relevance to the surveillance and control of TB: an e-debate. Infec. Gen. Evol.; 2: 153–158. Tibayrenc, M., Kjellberg, F., Arnaud, J., Oury, B., Brenière, S. F., Dardi, M-L. and Ayala, F. J. (1991). Are eukaryotic microorganisms clonal or sexual? A population genetics vantage. Proc. Natl. Acad. Sci. USA; 88: 5129-5133. Tibayrenc, M. and Ayala, F. (1988). Isoenzyme variability in Trypanosoma cruzi , the agent of Chagas disease: genetical, taxonomical and epidemiological significance. Evolution; 42: 277-292. Tibayrenc, M., Kjellberg, F. and Ayala, F. J. (1990). A clonal theory of parasitic protozoa: the population structure of Entamoeba , Giardia, Leishmania, Naegleria, Plasmodium, Tricamonas and Trypanosoma , and its medical and taxonomical consequences. Proc. Natl. Acad. Sci. USA; 87: 2414–2418. Tibayrenc, M., Neubauer, K., Barnabe, C., Guerrini, F., Skarecky, D., and Ayala, F. J. (1993). Genetic characterization of six parasitic protozoa: Parity between random-primerDNA typing and multilocus enzyme electrophoresis. Proc. Natl. Acad. Sci.; 90: 1335–1339. Tomimori-Yamashita, J., Nguyen, T. H., Maeda, S. M., Flageul, B., Rotta, O. & Cruaud, P. (1999). Anti-Phenolic Glycolipid-I (PGL-I) Determination Using Blood Collection On Filter Paper In Leprosy Patients.Rev. Inst. Med. trop. S. Paulo; 41 (4): 239-242. Travi, B. L., Osorio, Y., Melby, P. C., Chandrasekar, B., Arteaga, L., and Saravia. N. G. (2002). Gender Is a Major Determinant of the Clinical Evolution and Immune Response in Hamsters Infected with Leishmania spp. Infec. Immunity.; 70 (5) . 2288–2296. Tsaknaridis, L., Spencer, L., Culbertson, N., Hicks, K., LaTocha, D., Chou, Y, K., Whitham, R. H., Bakke, A., Jones, R. E., Offner, H., Bourdette, D. N. and Vandenbark, A.A. (2003).Functional assay for human CD4+CD25+ Treg cells reveals an age-dependent loss of suppressive activity. J. Neurosci. Res.;74: 296–308. Turelli, M., Barton, N. H. and Coyne, J. C. (2001). Theory and speciation. Trends Ecol. Evol..;16: 330-343. Valenzuela, J. G., Belkaid, Y., Garfield, M. K., Susana Mendez, S., Shaden Kamhawi, S., Rowton, E. D., Sacks, D. L. and Ribeiro, J. M. C. (2001).Toward a Defined Anti- Leishmania Vaccine Targeting Vector Antigens: Characterization of a Protective Salivary Protein. J. Exp. Med.;194 (3): 331-342. Van Eys, G. J. J. M.; Schoone, G. J.; Ligthart, G. S.; Alvar, J.; Evans, D. A. & Terpstra, W. J. (1989). Identification of ‘old world’ leishmaniasis by DNA recombinant probes. Mol. Biochem. Parasitol., 34: 53-62. Van Eys, G. J. J. M., G. J. Schoone, N. C. M. Kroon, and S. B. Ebeling. (1992). Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol. Biochem. Parasitol.; 51: 133–142. Vardy, D., Frankenburg, S., Goldenhersh M, Leibovici, V. & Klaus, S. N. (1993). Unusually extensive disease caused by Leishmania major parasites. Clin Exp. Dermatol.; 18: 36–40. Vega-Lopez, F. (2003). Diagnosis of cutaneous leishmaniasis. Curr. Opin. Infect. Dis.; 16: 97-101. Vermaat, M. B., Ion, R. A., Does, R. J. M. M. and Klaassen, C. A. J. (2003). A Comparison of Shewhart Individuals Control Charts Based on Normal, Non-parametric, and Extreme-value Theory. Qual. Reliab. Engng. Int.;19: 337–353. Victoir, K. & Dujardin, J. C. (2002). How to succeed in parasitic life without sex? Asking Leishmania. Trends Parasitol.; 18: 81 -85. Wakeley, J. (2005). The Limits of Theoretical Population Genetics. Genetics.169: 1–7. Wakeley, J. and Takahashi, T. (2003). Gene Genealogies When the Sample Size Exceeds the effective Size of the Population. Mol. Biol. Evol.; 20 (2): 208–213. Wasserberg, G., Abramsky, Z., Anders, G., El-Fari, M., Schoenian, G., Schnur, L., Kotler, B. P., Kabalo, I., Warburg, A. (2002). The ecology of cutaneous leishmaniasis in Nizzana, Israel: infection patterns in the reservoir host, and epidemiological implications. Int. J. Parasitol.; 32: 133–143. Wasserberg, G., Yarom, I. and A. Warburg, A. (2003). Seasonal abundance patterns of the sandfly Phlebotomus papatasi in climatically distinct foci of cutaneous leishmaniasis in Israeli deserts. Med. Vet. Entomol..;17, 452–456. Weber, J. L. and Wong, C. (1993). Mutation of human short tandem repeats. Hum. Mol. Genet.; 2: 1123-1128. Weigle, K. A., de Davalos, M., Heredia, P. Molineros, R., Saravia, N. G. and D’Alessandro, A. (1987). Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am. J. Trop. Med. Hyg.; 36: 489–496. Weigle, K. A., Labrada, L. A., Lozano, C. Santrich, C. and Barker, D. C. (2002). PCR-based diagnosis of acute and chronic cutaneous leishmaniasis caused by Leishmania (Viannia). J. Clin. Microbiol.; 40: 601–606. Weir, B. S, Cockerham, C. C. (1984). Estimating F-statistics for the analysis of population structure. Evolution;38: 1358-1370 Westgard, J. O., Barry, P.L., Hunt, M.R. and Groth, T. (1981). A multi-rule Shewhart chart for quality control in clinical chemistry. Clin Chem.; 27: 493-501. WHO. (1990). Expert Committee: Control of the Leishmaniasis. WHO Techn. Ser.793., Geneva. WHO. (1999). Recommended Surveillance Standards, Second edition, October 1999. WHO/CDS/CSR/ISR/99.2. WHO. (1991). Information on the Epidemiology and Control of the Leishmaniasis by Country or Territory. WHO/Leish/91, Geneva. WHO. (1996). Manual on Visceral Leishmaniasis Control. WHO/6531–6535. Leish/96–40, Geneva. WHO. (1998). Leishmania and HIV in gridlock. WHO/UNAIDS report, 12–25, Geneva. WHO (2002). Cutaneous leishmaniasis, Afghanistan. Wkly. Epidemiol. Rec.; 29: 246. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rabalski, J. A., and Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res.; 18: 6531–6535. Wilson, J. F., Weale, M. E., Smith, A. C., Gratrix, F., Fletcher, B., Thomas, M. G., Bradman, N., Goldstein, D. B. (2001). Population genetic structure of variable drug response. Nature Genet;. 29 (3): 265-269. Wilson, S. M. (1995). DNA-based methods in the detection of Leishmania parasites: Field applications and practicalities. Ann. Trop. Med. Parasitol.; 89 (1): 95-100. Wright, S. (1978). Evolution and the genetics of natural populations: Variability within and among natural populations. University of Chicago Press, Chicago. vol 4. You, C. L., Korol, A. B., Tzion, F., Beiles, A. and Nevo, E. (2002). Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol. Ecol.; 11: 2453-2465. Zaykin, D., Zhivotovsky and Weir, B. S. (1995). Exact tests for association between alleles at arbitrary numbers of loci. Genetica; 96: 169-179. Zhivotovsky, L. A., Bennett, L., Bowcock, A. M. and Feldman, M. W. (2000). Human population expansion and microsatellite variation. Mol. Biol. Evol.; 7: 757–767.