Characterization of the specificity of human neutrophil elastase for Shigella flexneri virulence factorsPetra Averhoff111.11.1.121.1.21.1.334562#3641.1.451.21.2.161.2.271.2.38427#45591.3101.41.4.1111.4.2436#36712360#8613421#16114408#39615454#1101.4.31617466#470181.5192192.12.1.1202.1.22.1.3212.1.42.22.2.12.2.2222.2.32.2.42.32.3.1232.3.22.3.32.3.4242.3.52.42.52.5.1252.5.22.5.3262.5.4272.62.6.1282.6.22.6.3292.72.7.12.7.22.7.3302.7.4312.7.52.8323323.13.1.133527#15534627#2113.1.235474#1573.2363.2.137437#19238409#2823.2.23940493#2783.2.341582#36842302#15243486#190443.2.4561#27645593#5503.2.546546#20847477#36148550#27649351#2583.2.650570#1935152530#54853582#4304544.14.1.14.1.255564.24.2.14.2.257 NE mutants 35-41 and 58A-61370#4175859NE mutants F192A, F192K and A213V60361#34161NE mutants F215Y and F215A 62395#381NE mutants 216-218 and 216-22463406#41964NE mutant N98L and N98A65528#4194.2.3665665.1575#3435.2636#630491#5645.3593#6615.4488#619464#291329#145405#288AbbreviationsReferencesCurriculum Vitae PublicationsAcknowledgementsSelbständigkeitserklärungenTable of contentsHelp Abbreviations

The abbreviations for the individual amino acids are listed in chapter 5.4.

aa

216-219

216-224

35-41

59-61

BPI

CAMP

CG

DSMZ

F192A

F192K

F215A

F215Y

FCS

fMLP

HBSS

HEPES

hNGE

IC

IcsA

IL-8

Il-18

IL-1β

IpaB

LPS

MALDI

MeO

min

MS

N98A

N98L

NE

NE-CMK

NETs

nm

nt

OmpA

PAMPs

PBS

pCG

pI

PMA

PMSF

pNE

PRRs

RMSD

ROS

RPMI

RT

RZPD

SDS

SDS-PAGE

sec

Suc

TCA

TLR

TNF-α

TOF

V213A

V

Wt

Z-GLF-CMK

amino acid

NE mutant VRGG216-219GKSS

NE mutant VRGGGSLY216-224GKSSGVP

NE mutant LRRGHF35-41IQSPAGQSR

NE mutant VANVNVR59-61WGSNINV

bactericidal permeability increasing protein

cationic antimicrobial peptides

cathepsin G

Deutsche Sammlung für Mikroorganismen und Zellkulturen

NE mutant F192A

NE mutant F192K

NE mutant F215A

NE mutant F215Y

foetal calf serum

aminoterminal formylated methionin bacterial peptide

Hank’s balanced salt solution

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic a

human neutrophil granule extract

inhibitor cocktail

intracellular spreading A

interleukin 8

interleukin 18

interleukin 1β

invasion plasmid antigen B

lipopolysaccharides

matrix assisted laser desorption ionisation

methoxy-

minutes

mass spectrometry

NE mutant -N98A

NE mutant N98L

neutrophil elastase

NE inhibitor; MeO-Suc-AAPV-chloromethyl ketone

neutrophil extracellular traps

nanometer

non-transfected

outer-membrane protein A

pathogen associated molecular patterns

phosphate-buffered saline

commercially available purified human CG

isoelectric point

phorbol 12-myristate 13-acetate PMA

phenylmethylsulphonylfluoride

commercially available purified human NE

pathogen recognition receptors

root mean square standard deviation

reactive oxygen species

Roswell Park Memorial Institute

room temperature

German Resource Center for Genome Research

sodium dodecyl sulphate

SDS polyacrylamide gel electrophoresis

seconds

succinyl-

trichloroacetic acid

toll like receptors

tumor necrosis factor α

time of fligt

NE mutant V213A

vector

recombinant wildtype NE

CG inhibitor; benzyloxycarbonyl –GLF-chloromethyl ketone