Bridg, Hannia: Micropropagation and Determination of the in vitro Stability of Annona cherimola Mill. and Annona muricata L.

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Chapter 8. Results
Karyotype observations

The observation of mitotic chromosomes under the light microscope is a rapid and informative method of studying the genomes as a whole. Although the technique, involving the collection, fixation, staining and preparation of chromosome squashes, is easy, the resolution of the A. cherimola and A. muricata chromosomes were not clear and many observations were required.

The tested methods to visualize the chromosomes of A. cherimola and A. muricata were ineffective. Only the DAPI-fluorescence method allowed the visualization of the chromosomes but with some difficulties (Figure 29).

The A. cherimola and A. muricata showed the tendency to clump or stick to one another. A few chromosones were oblique or vertical, this limitation was also reported for Annona spp. by Bowden (1948).

The number of chromosomes counted were diploid, the A. cherimola reported 2n=16; 14 and for A. muricata 2n=14; 18 (Figure 30).

Figure 31. Chromosomes of A. cherimola and A. muricata in vitro regenerants


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