The role of the Met tyrosine kinase receptor in skin maintenance and regenerationJolanta Chmielowiec1Zusammenfassung2Introduction Wound healing in the skin 23534#4274551#2335Mammalian skin6534#328The tyrosine kinase receptor Met7Met signal transduction8534#372534#6089550#226Met signalling during development10534#20111Met function in the adult The aim of the study12ResultsExpression of Met and HGF/SF in the skin and during skin wound healing12538#18913451#190Generation of mice deficient in Met in keratinocytes 14476#38215393#294Met signaling during generation and maintenance of the skin16382#658507#43417Wound closure in conditional Met mutant mice18380#672390#14819534#69620584#554Contribution of cells in the hyperproliferative epithelium21436#419481#30022Scratch-wound healing of Met mutant keratinocytes in cell culture585#21323587#47424612#376Cytoskeleton rearrangement in cultured scratchwounded keratinocytes 476#94125Signal transduction in primary keratinocytes26586#731Discussion26Conditional mutagenesis to investigate Met function in the skin27The role of the tyrosine kinase receptor Met in the skin28Only non-recombined cells contribute to wound healing29The role of HGF/SF and Met in development and regeneration30Only Met-positive keratinocytes contribute to healing of scratch-wounds in vitro31The Met receptor as a therapeutically targetMaterials and Methods32Extraction and purification of DNA3334Polymerase chain reaction (PCR)Southern blotting35Cell culture36Wounding of skinImmunhistochemical techniques37383940414243Protein biochemistry 4445534#216464748AbbreviationsReferencesErklärungPublicationsenTable of contentsHelp References 1. 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