Gentransfer in korneale Endothelzellen: nicht-virale und virale Ansätze zur Modulation der Endothelzellfunktion und allogenen Immunreaktion Dipl. Biologin Haike Dannowski geboren am 29.03.1973 in Rüdersdorf bei Berlin ###+#1.###+#1.1.325.5pt#226.5pt###+#1.1.1.307.5pt#272.25pt###+#1.2.###+#1.2.1.###+#1.2.2.338.25pt#226.5pt###+#1.2.3.###+#1.2.4.###+#1.2.5.###+#1.2.5.1.453.75pt#378.75pt###+#1.2.5.2.###+#1.2.5.3.357pt#226.5pt###+#1.2.5.4.453.75pt#157.5pt###+#1.2.6.###+#1.2.7.###+#1.2.7.1.###+#1.2.7.2.###+#1.2.7.3.###+#1.2.7.4.###+#2.###+#2.1.###+#2.1.1.###+#2.1.1.1.###+#2.1.1.2.###+#2.1.1.2.1.###+#2.1.1.2.2.###+#2.1.1.2.3.###+#2.1.1.2.4.###+#2.1.1.2.5.###+#2.1.1.2.6.###+#2.1.2.###+#2.1.2.1.###+#2.1.2.2.###+#2.1.3.###+#2.1.3.1.###+#2.1.3.1.1.###+#2.1.3.1.2.###+#2.1.3.1.3.###+#2.1.3.2.###+#2.1.3.2.1.###+#2.1.4.###+#2.1.4.1.###+#2.1.4.2.###+#2.1.4.3.###+#2.1.5.###+#2.1.5.1.###+#2.1.5.1.1.###+#2.1.5.1.2.340.5pt#221.25pt###+#2.1.5.2.###+#2.1.5.3.###+#2.1.5.4.###+#2.1.5.5.###+#2.1.5.5.1.###+#2.1.5.5.2.###+#2.1.5.5.3.###+#2.1.5.6.###+#2.1.6.###+#2.2.###+#2.2.1.###+#2.2.1.1.###+#2.2.1.2.###+#2.2.1.3.###+#2.2.1.4.###+#2.2.1.5.###+#2.2.1.6.###+#2.2.1.7.###+#2.2.1.8.###+#2.2.2.###+#2.2.3.###+#2.2.4.###+#2.2.5.###+#2.2.6.###+#2.2.7.###+#2.2.8.###+#2.2.9.###+#2.2.10.###+#2.2.10.1.###+#2.2.10.2.###+#2.2.11.###+#2.2.12.###+#2.2.13.###+#3.###+#3.1.###+#3.1.1.###+#3.1.1.1.453.75pt#472.5pt###+#3.1.1.2.453.75pt#629.25pt###+#3.1.2.453.75pt#528.75pt453.75pt#569.25pt###+#3.1.3.453.75pt#310.5pt###+#3.2.###+#3.2.1.###+#3.2.1.1.284.25pt#139.5pt453.75pt#180.75pt###+#3.2.1.2.368.25pt#204.75pt###+#3.2.1.3.###+#3.2.1.4.###+#3.2.1.5.###+#3.2.1.5.1.453.75pt#174.75pt453pt#224.25pt###+#3.2.1.6.453pt#232.5pt###+#3.2.1.7.339pt#170.25pt453.75pt#414.75pt374.25pt#177.75pt###+#3.2.1.8.453.75pt#387.75pt453.75pt#387.75pt###+#3.2.2.###+#3.2.2.1.399.75pt#261pt###+#3.2.2.2.453pt#222pt###+#4.###+#4.1.###+#4.1.1.###+#4.1.1.1.###+#4.1.1.2.###+#4.1.1.3.###+#4.1.1.4.###+#4.1.1.5.###+#4.2.###+#4.2.1.###+#4.2.1.1.###+#4.2.2.###+#4.3.###+#5.AbkürzungsverzeichnisAnhang Danksagung SelbstständigkeitserklärungdeInhaltsverzeichnisHilfe Anhang Publikationen und Kongressbeiträge

Publikationen:

Pleyer U,Dannowski H,Volk HD, Ritter T (2001).Corneal allograft rejection: current understanding. I. Immunobiology and basic mechanisms.Ophthalmologica.215:254-262. Review

Pleyer U,Dannowski H, Reszka R, Volk HD, Hartmann C, Ritter T (2001).Gene therapy in ophthalmology.Review of options and trends in corneal diseases. Klin Monatsbl Augenheilkd. 218:140-147. Review.German.

Pleyer U,Dannowski H(2002).Delivery of genes via liposomes to corneal endothelial cells.Drug News Perspect 15:283-289.

Mergler S,Dannowski H, Bednarz J, Engelmann K, Hartmann C, Pleyer U (2002).Calcium influx induced by activation of receptor tyrosine kinases in human corneal endothelial cells.Exp Eye Res77:485-495.

Dannowski H,Bednarz J, Reszka R, Engelmann K, Pleyer U (2004).Liposomal and non-liposomal gene transfer of acidic fibroblast growth Factor (aFGF) into human corneal endothelial cells (HCEC).(zur Veröffentlichung eingereicht bei Exp Eye Res)

Yang J, Pleyer U,Dannowski H, Vogt K, Hempel M, Volk HD, Ritter T (2004).Effects of local interleukin-12p40 gene therapy on rat corneal allograft survival.(zur Veröffentlichung eingereicht).


Kongressbeiträge; Vorträge* und Poster

ARVO 2001 Annual Meeting in Fort Lauderdale, Florida, USA (29.4.-4.5.2001)

Dannowski H*, Bednarz J, Reszka R, Engelmann K, Pleyer U (2001).Liposomal and nonliposomal Gene Transfer of Acidic Fibroblast Growth Factor (aFGF) into Human Corneal Endothelial Cells (HCEC).InvestOphthalmol Vis Sci. 42:105.

Mergler S, Dannowski H, Engelmann K, Pleyer U (2001). Intracellular calcium increase mediated by acidic (aFGF) and basic (bFGF) fibroblast growth factors in SV 40 transfected cultured human corneal endothelial cells. Invest Ophthalmol Vis Sci. 42:274.

99. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft, Berlin (29.9.-2.10.2001)

Dannowski H*, Bednarz J, Reszka R, Engelmann K, Pleyer U. Der Effekt von Lipid-vermitteltem Gentransfer des sauren Fibroblasten-Wachstumsfaktors (aFGF) auf die Proliferation humaner kornealer Endothelzellen (HCEC) in vitro.Ophthalmologe 22:Suppl.1.

ARVO 2002 Annual Meetingin Fort Lauderdale,Florida,USA (5.5.-10.5.2002)

Sedlakova K,Dannowski H,Brandt C, Schmidt N, Filipec M, Hartmann C, Ritter T, Pleyer U (2002).Adenovirus-Mediated Gene Transfer of vIL-10 in Experimental Corneal Transplantation.Invest Ophthalmol Vis Sci 43: E-Abstract 2279.

Mergler S,Dannowski H, Bednarz J, Engelmann K, Hartmann C, Pleyer U (2002).Electrophysiological properties in cultured human corneal endothelial cells.Invest Ophthalmol Vis Sci. 43: E-Abstract 3138.

100. Jahrestagung derDeutschen Ophthalmologischen Gesellschaft, Berlin (26.9.-29.9.2002)

Mergler S,Dannowski H, Bednarz J, Engelmann K, Hartmann C, Pleyer U (2002). Elektrophysiologische Untersuchungen an Zellkulturen des humanen Hornhautepithels. Ophthalmologe 191:Suppl.1.


EVER 2002 Joint European Research Meeting in Ophthalmology and Vision, Alicante, Spanien (2.10.-5.10.2002)

Schmidt N,Dannowski H, Sedlakova K, Ritter T, Pleyer U (2002).Immunohistological investigation of infiltrating cells after viral interleukin-10 transduction in experimental keratoplasty.Ophthalmic Res 34:Suppl.1:39.

ARVO 2003 Annual Meeting in Fort Lauderdale,Florida,USA (4.5.-9.5.2003)

Dannowski H,Bednarz J, Reszka R, Engelmann K, Pleyer U (2003).Lipid-mediated Gene Transfer of EGFP into Human Corneal Endothelial Cells (HCEC). Invest Ophthalmol Vis Sci. 44: E-Abstract 845.

Schmidt N,Dannowski H, Sedlákova K, Ritter T, Pleyer U (2003).Immunohistological Investigation of Infiltrating Cells after Viral Interleukin-10 Transduction in Experimental Keratoplasty. Invest Ophthalmol Vis Sci. 44: E-Abstract 4659.

Yang J, Pleyer U,Dannowski H, Vogt K, Hempel M, Ritter T (2003).Effects of Adenovirus-mediated Gene Transfer of IL-12p40 on Corneal Allograft Survival. Invest Ophthalmol Vis Sci. 44: E-Abstract 4656.

Mergler S,Dannowski H, Bednarz J, Engelmann K, Hartmann C, Pleyer U (2003). L-type Ca2+ channel activity in cultured human corneal endothelial cells.Invest Ophthalmol Vis Sci. 44: E-Abstract 4728.

IOIS - VII international Symposium on ocular inflammation, Padova, Italien (25.5.–30.5.2003)

Pleyer U, Yang J,Dannowski H, Ritter T (2003).Effect of interleukin-12p40 on experimental keratoplasty.

101. Jahrestagung der Deutschen Ophthalmologischen Gesellschaft, Berlin (25.9.-28.9.2003)

Mergler S,Dannowski H, Bednarz J, Engelmann K, Hartmann C, Pleyer U (2003).Aktivierung von L-Typ Ca2+-Kanälen in kultivierten humanen kornealen Endothelzellen über Fibroblastenwachstumsfaktoren (FGF): Unterschiede zwischen a-FGF und b-FGF. Ophthalmologe 100:Suppl.1

34. Jahrestagung der Deutschen Gesellschaft für Immunologie, Berlin (24.9.-27.9.2003)

Yang J, Pleyer U,Dannowski H, Vogt K, Hempel M, Volk HD, Ritter T (2003).Effect of adenovirus-mediated gene transfer of interleukin-12p40 on corneal allograft survival.