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Abbreviations


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A

 
  

Ab

antibody

AdoHCy

S-adenosyl-L-homocysteine

AdoMet

S-adenosyl-L-methionine

ADP

adenosine diphosphate

Ar

argon

5AzaC

5-azacytidine

  

B

 
  

BAH

bromo adjacent homology domain (also called PBHD)

BDGP

Berkeley Drosophila Genome Project

BLAST

Basic Local Alignment Search Tool

bp

base pairs

BrdU

5-bromodeoxyuridine

  

C

 
  

C2C12

mouse myoblast cell line

CaPO4

calcium phosphate

CCD

charge coupled device

cdk2

cyclin dependent kinase-2

CENP-B

centromere protein B

CMT

chromomethyltransferase

COS-7

african green monkey kidney fibroblast-like cells transformed with SV40 T antigen

CpA

cytosine-adenine doublet

CpG

cytosine-guanine doublet

Cy5

indodicarbocyanine

  

D

 
  

DABCO

1,4-diazabicyclo[2,2,2]-octane

DIC

differential interference contrast

DMAP

DNMT1-associated protein

DmDNA Ligase I

Drosophila melanogaster DNA Ligase I

DMEM

Dulbecco's Modified Eagle Medium

DmPCNA

Drosophila melanogaster PCNA

DMSO

dimethyl sulfoxide

DNA

deoxyribonucleic acid

DNA MTase

DNA methyltransferase

DNase

deoxyribonuclease

DsRed

red fluorescent protein from Discosoma species

  

E

 
  

EC

embryonic carcinoma

EDTA

ethylenediaminetetraacetic acid

ES

embryonic stem cells

EST

expressed sequence tag

E-value

expectation value

 

F

 
  

FCS

fetal calf serum

FITC

fluorescein isothiocyanate

  

G

 
  

G1

gap 1 phase

G2

gap 2 phase

G418

geneticin

GFP

green fluorescent protein

  

H

 
  

H167V

mutation of histidine at position 167 to valine in DNMT1

H2A

histone H2A

H2B

histone H2B

H3

histone H3

H3K9Me

histone H3 methylated at lysine at position 9

H4

histone H4

HDAC

histone deacetylase

HeNe

helium neon

HP-1

heterochromatin protein 1

hr

hours

HsDNA Ligase I

human DNA Ligase I

HsPCNA

human PCNA

  

I

 
  

ICF

immunodeficiency, centromeric instability and facial anomalies

  

K

 
  

kbp

kilobase pairs

kDa

kilo Dalton

  

M

 
  

M

mitosis

MBD

methyl-CpG-binding domain

Mbp

megabase pairs

5mC

C5-methylcytosine

MeCP

methyl-CpG-binding protein

mM

millimolar

  

N

 
  

neo r

neomycin resistance gene

NLS

nuclear localization signal

NuRD

nucleosome remodelling and histone deacetylation complex

  

P

 
  

PAGE

polyacrylamide gel electrophoresis

PBD

PCNA binding domain

PBHD

polybromo homology domain (also called BAH domain)

PBS

phosphate buffered saline

PCNA

proliferating cell nuclear antigen

PCR

polymerase chain reaction

pol

DNA polymerase

PSI-BLAST

position-specific iterated BLAST

PVDF

polyvinylidene fluoride

  

R

 
  

RF

replication foci

RFP

red fluorescent protein

RFTS

replication factory targeting sequence

RPA-70

70 kDa subunit of replication protein A

  

S

 
  

S

Synthesis phase

S2

Drosophila Schneider’s line 2 cells

S514A

mutation of serine at position 514 to alanine in DNMT1

S514D

mutation of serine at position 514 to aspartate in DNMT1

SAH

S-adenosyl-L-homocysteine

SAM

S-adenosyl-L-methionine

ScPCNA

Saccharomyces cerevisiae PCNA

SDS

sodium dodecylsulphate

SV40

simian virus 40

  

T

 
  

293T

human embryonic kidney cells transformed with SV40 T antigen

TR

texas red

Tris

tris (hydroxymethyl) aminomethane

TS

targeting sequence

  
  
  

W

 
  

WSTF

Williams syndrome transcription factor

  

Y

 
  

YFP

yellow fluorescent protein

  

Z

 
  

Zn

zinc

  

ß

 
  

ß-gal

betagalactosidase epitope

  

µ

 
  

µm

micrometer

µM

micromolar


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