Abbreviations

A. bidest

Aqua bidestillata

 

AIF

apoptosis inducing factor

ANT

Adenine Nucleotide transporter

Apaf-1

apoptosis associated factor1

APS

Ammoniumperoxodisulfate

Bak

Bcl-2 homologous antagonist killer

Bap31

Bcl-2-associated protein-31

Bax

Bcl-2 associated protein X

BCA

Bicinchonin acid

Bcl-2

B-cell lymphoma / leukemia-2 gene

BGHpolyA

Bovine growth hormone polyadenylation signal

BH

Bcl-2 homology

Bik/Nbk

Bcl-2 interacting killer/Natural born killer

BSA

Bovines serum Albumin

CAPS

3-Cyclohexylamino-1-propansulfon acid

CARD

caspase recruitment domain

CMV

Cytomegalovirus

ColE1 ori

High-copy number origin of replication for E. coli

C-Terminus

carboxy-terminus

CVL

crude virus lysate

Cyt c

cytochrome c

Da

Dalton

DEAE

Diethylaminoethanol

DD

death domain

DED

death effector domain

Diablo

Direct IAP binding protein with low pI

DISC

death inducing signalling complex

DMSO

Dimethylsulfoxid

DNA

Desoxyribonucleic acid

dNTP

2´-Desoxynukleosidtriphosphat

∆Ψm

mitochondrial membrane potential

DTT

Dithiothreitol

E. coli

Escherichia coli

ECL

Enhanced chemoluminescence

EDTA

Ethylendiamine-tetraactylacid

ER

endoplasmic reticulum

EtBr

Ethidiumbromide

EtOH

ethanol

FBS

fetal bovine serum

FITC

Fluorescein-isothiocyanat

fmk

Fluoromethylketon

FSC

Forward scatter

h

hour

HEPES

2-[4-(2-Hydroxyethyl)-1-piperazinyl]-Ethansulfonic acid

HRP

horse radish peroxidase

HtrA2

High temperature requirement A2

IAP

inhibitor of apoptosis protein

IRES

Internal Ribosome Entry Site

JC-1

5,5‘,6,6‘tetrachloro1,1‘,3,3‘tetraethylbenzimidazolyl

carbocyaniniodide

LB-medium

Luria-Browth-Medium

MEF

Murine embryonic fibroblasts

min

minute

MOI

multiplicity of infection

MTS

Mitochondrial targeting sequence

N-Terminus

Amino-terminus

PAGE

Polyacrylamide gel electrophoresis

PARP

Poly(ADP-ribose) Polymerase

PBS

Phosphate buffered saline

PCMV

full length “immediate early” promotor of cytomegalovirus

PCR

Polymerase chain reaction

Pfu

Plaque forming unit

PI

propidium iodide

PIDD

p53-induced protein with Death Domain

PminiCMV

minimal “immediate early” promotor of cytomegalovirus

PMSF

Phenylmethylsulfonylfluoride

PTP

permeability transition pore

RAIDD

RIP-associated ICH-1/CED-3-homologous protein with Death

Domain

RING-domain

Really Interesting New Gene

Rpm

rounds per minute

RT

room temperature

s

Second

SDS

Sodium dodecylsulfate

SERCA

Sarco(Endo)plasmatic reticulum Ca2+-ATPase

Smac

second mitochondria activator of caspases

SSC

Side scatter

TAE-buffer

Tris-Acetat-EDTA-buffer

TEMED

N, N, N‘, N‘-Tetramethylethylendiamin

TM

Transmembrane domain

TNF

tumour necrosis factor

TRE

Tetracycline-responsive element

Tris

Tris-(hydroxymethyl)-aminomethan

tTA

Tetracycline-controlled transactivator

U

Unit

UPR

unfolded protein response

V

Volt

v/v

volume / volume

VDAC

voltage dependent anion channel

Vol.

Volume

w/v

weight / volume

XIAP

X-chromosome-linked Inhibitor of Apoptosis Protein

X-Gal

5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranosid


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