A

Adenin

AAP

`Abridged Anchor Primer´

Abb.

Abbildung

AdMLP

Adenovirus Major Late Promotor

Aqua bidest.

zweifach destilliertes Wasser

et al.

et altera

amp^R

Ampicillinresistenz

amt

Adenin-Methyltransferase Gen

ATP

Adenosintriphosphat

AUAP

`Abridged Universal Amplification Primer´

bla

β -Lactamase Gen

bp

Basenpaare

BRE

`IIB recognition element´

BSA

`bovine serum albumin´ (Rinderserumalbumin)

C

Cytosin

CA

Chloramphenicol

CaMV

Cauliflower Mosaic Virus

cat

Chloramphenicol-Acetyltransferase Gen

ccc

`covalently closed circular´ (superhelikal)

cDNA

komplementäre Desoxyribonukleinsäure

CIAP

`Calf Intestine Alkaline Phosphatase´

cm

Zentimeter

CsCl

Caesiumchlorid

cv.

cultivar

°C

Grad Celsius

dC

Desoxycytosin

dCTP

Desoxycytosintriphosphat

DEPC

Diethyl-pyrocarbonat

DMSO

Dimethylsulfoxid

DNA

Desoxyribonukleinsäure

dNTP

Desoxynukleosidtriphosphat

DPE

`downstream promoter element´

DTNB

5,5´-Dithiobis-2-nitrobenzoesäure

DTT

1,4-Dithiothreitol

EDTA

Ethylendiamintetraacetat

EtOH

Ethanol

FA-Gelpuffer

Formaldehyd-Gelpuffer

Fam

6-Carboxy-Fluorescein

g

Gramm

G

Guanin

GTF

`general transcription factor´

GSP

genspezifischer Primer

gus

β-Glucuronidase Gen

GVO

gentechnisch veränderter Organismus

h

Stunde(n)

HGT

horizontaler Gentransfer

I

Inosin

Inr

Initiator

IPTG

Isopropyl-β-D-thiogalactopyranosid

kbp

kilo Basenpaare

KCl

Kaliumchlorid

kDa

kilo Dalton

km^R

Kanamycinresistenz

kV

Kilovolt

l

Liter

LB

`left border´ (linke Grenze)

LB-Medium

Luria Bertani-Medium

M

Molar

MCS

` multiple cloning site´

mg

Milligramm

MgCl₂

Magnesiumchlord

min

Minute(n)

ml

Milliliter

MOPS

Morpholinopropansulfonsäure

ms

Millisekunden

µF

Mikrofarrad

µg

Mikrogramm

µl

Mikroliter

N

Adenin, Cytosin, Guanin oder Thymin

NEP

`nuclear-encoded plastid RNA polymerase´

ng

Nanogramm

nm

Nanometer

nm^R

Neomycinresistenz

mRNA

messenger Ribonukleinsäure

nos

Nopalin-Synthase Gen

npt II

Neomycin-Phosphotransferase Gen II

npt III

Neomycin-Phosphotransferase Gen III

nt

Nukleotid

ocs

Octopin-Synthase Gen

OD

optische Dichte

Ω

Ohm

P

Promotor

PCR

`polymerase chain reaction´ (Polymerase-Kettenreaktion)

PEP

`plastid-encoded plastid RNA polymerase´

pg

Pikogramm

PIC

Prä-Initiationskomplex

Pu

Purinbase

Py

Pyrimidinbase

R

Adenin oder Guanin

RACE

`Rapid Amplification of cDNA Ends´

RB

`right border´ (rechte Grenze)

rif^R

Rifampicinresistenz

RLU

`relative light units´ (Relative Lichteinheiten)

RNA

Ribonukleinsäure

RNAP

DNA-abhängige RNA Polymerase

Rox

6-Carboxy-X-rhodamin

rpm

`rounds per minute´ (Umdrehungen pro Minute)

rRNA

ribosomale RNA

RT

Raumtemperatur

RT´

Reverse Transkriptase

s

Sekunde(n)

SLF

`sigma-like factor´

snRNA

`small nuclear´ RNA

T

Thymin

Tab.

Tabelle

TAF

TBP assoziierter Faktor

Tamra

6-Carboxy-tetramethyl-rhodamin

Taq

Thermus aquaticus

TBP

TATA-Bindungsprotein

tc^R

Tetrazyklinresistenz

T-DNA

`transfer´ DNA

TdT

Terminale Desoxynukleotidyl-Transferase

Temp.

Temperatur

TF

Transkriptionsfaktor

TFTC

`TBP-free TAF_II -containing complex´

Ti-Plasmid

`tumor inducing plasmid´

TLF

`TBP-like factor´

T_m

Schmelztemperatur

Tn

Transposon

TRF

`TBP-related factor´

Tris

Trishydroxymethylaminomethan

tRNA

`transfer´ RNA

TY

Trypton Yeast

U

`unit´ (Einheit)

UE(s)

Untereinheit(en)

ü. N.

über Nacht

UAS

`upstream activating sequences´

UTR

`untranslated region´

UV

ultraviolett

V

Volt

var.

Varietät

W

Adenin oder Thymin

WT

Wildtyp

X-Gluc

5-Brom-4-chlor-3-indolyl-β -D-glucuronid

Y

Cytosin oder Thymin