<?xml version="1.0" encoding="ISO-8859-1"?><cms:container xmlns:cms="http://edoc.hu-berlin.de/diml/module/cms"><cms:document><cms:meta><cms:entry id="front" part="front" ref="front" type="front"/><cms:entry type="title">Sequentielle Genotypisierung von <em>Pseudomonas aeruginosa</em>-Isolaten und Übereinstimmung von bakteriologischen Proben aus dem oberen und unteren Respirationstrakt von Patienten mit Cystischer Fibrose</cms:entry><cms:entry type="author">Andreas  Jung</cms:entry><cms:entry id="N10040" part="front" ref="N10040" type="dedication"/><cms:entry id="chapter1" part="chapter1" ref="chapter1" type="chapter">1 Einleitung</cms:entry><cms:entry id="N100D1" part="chapter1" ref="N100D1" type="section">1.1 Stand der Forschung</cms:entry><cms:entry id="N100D6" part="chapter1" ref="N100D6" type="subsection">1.1.1 Cystische Fibrose: Historisches</cms:entry><cms:entry id="N100DD" part="chapter1" ref="N100DD" type="citenumber">1</cms:entry><cms:entry id="N10118" part="chapter1" ref="N10118" type="subsection">1.1.2 Pathophysiologie der Atemwege</cms:entry><cms:entry id="N10126" part="chapter1" ref="N10126" type="citenumber">2</cms:entry><cms:entry id="N10146" part="chapter1" ref="N10146" type="subsection">1.1.3 Pulmonale Infektion mit P. aeruginosa
               </cms:entry><cms:entry id="N1018D" part="chapter1" ref="N1018D" type="subsection">1.1.4 Mikrobiologische Probengewinnung</cms:entry><cms:entry id="N101CE" part="chapter1" ref="N101CE" type="subsection">1.1.5 Typisierung von P. aeruginosa
               </cms:entry><cms:entry id="N101D8" part="chapter1" ref="N101D8" type="citenumber">3</cms:entry><cms:entry id="N102AD" part="chapter1" ref="N102AD" type="section">1.2 Aufgabenstellung</cms:entry><cms:entry id="N102B4" part="chapter1" ref="N102B4" type="citenumber">4</cms:entry><cms:entry id="chapter2" part="chapter2" ref="chapter2" type="chapter">2 Material und Methoden</cms:entry><cms:entry id="N102CB" part="chapter2" ref="N102CB" type="section">2.1 Patientenkollektiv, Ein- und Ausschlusskriterien</cms:entry><cms:entry id="N102D0" part="chapter2" ref="N102D0" type="helpercitenumber">4</cms:entry><cms:entry id="N102F1" part="chapter2" ref="N102F1" type="section">2.2 Probenentnahme</cms:entry><cms:entry id="N102F6" part="chapter2" ref="N102F6" type="subsection">2.2.1 Untersuchungszeitpunkte, Art der Proben</cms:entry><cms:entry id="N102FD" part="chapter2" ref="N102FD" type="citenumber">5</cms:entry><cms:entry id="N10308" part="chapter2" ref="N10308" type="subsection">2.2.2 Bronchiallavage</cms:entry><cms:entry id="N10315" part="chapter2" ref="N10315" type="subsection">2.2.3 Mikrobiologische Anzucht und Identifizierung</cms:entry><cms:entry id="N10323" part="chapter2" ref="N10323" type="citenumber">6</cms:entry><cms:entry id="N10329" part="chapter2" ref="N10329" type="section">2.3 Genotypisierung (Genetisches Fingerprinting)</cms:entry><cms:entry id="N1032E" part="chapter2" ref="N1032E" type="subsection">2.3.1 Phenol-Chlorophorm-Extraktion der genomischen DNA</cms:entry><cms:entry id="N1033A" part="chapter2" ref="N1033A" type="subsection">2.3.2 Random Amplified Polymorphic DNA (RAPD)-Analyse</cms:entry><cms:entry id="N1037C" part="chapter2" ref="N1037C" type="citenumber">7</cms:entry><cms:entry id="N1037F" part="chapter2" ref="N1037F" type="table"/><cms:entry id="N10690" part="chapter2" ref="N10690" type="subsection">2.3.3 DNA-Makrorestriktion und Pulsfeld-Gelelektrophorese</cms:entry><cms:entry id="N10697" part="chapter2" ref="N10697" type="citenumber">8</cms:entry><cms:entry id="N106BA" part="chapter2" ref="N106BA" type="section">2.4 Auswertung der Ergebnisse und Biometrie</cms:entry><cms:entry id="N106C1" part="chapter2" ref="N106C1" type="citenumber">9</cms:entry><cms:entry id="chapter3" part="chapter3" ref="chapter3" type="chapter">3 Ergebnisse</cms:entry><cms:entry id="N106D2" part="chapter3" ref="N106D2" type="section">3.1 Mikrobiologische Kulturen und Lungenfunktionsdaten</cms:entry><cms:entry id="N106D7" part="chapter3" ref="N106D7" type="helpercitenumber">9</cms:entry><cms:entry id="N106E2" part="chapter3" ref="N106E2" type="citenumber">10</cms:entry><cms:entry id="N106E5" part="chapter3" ref="N106E5" type="table"/><cms:entry id="N107E7" part="chapter3" ref="N107E7" type="citenumber">11</cms:entry><cms:entry id="N107EA" part="chapter3" ref="N107EA" type="table"/><cms:entry id="N1106E" part="chapter3" ref="N1106E" type="section">3.2 Vorhersagewerte der Atemwegsproben</cms:entry><cms:entry id="N11078" part="chapter3" ref="N11078" type="citenumber">12</cms:entry><cms:entry id="N1107B" part="chapter3" ref="N1107B" type="table"/><cms:entry id="N111A5" part="chapter3" ref="N111A5" type="section">3.3 Vergleich der Typisierungsmethoden</cms:entry><cms:entry id="N111BB" part="chapter3" ref="N111BB" type="citenumber">13</cms:entry><cms:entry id="N111BE" part="chapter3" ref="N111BE" type="table"/><cms:entry id="N11335" part="chapter3" ref="N11335" type="mm">605#657</cms:entry><cms:entry id="N11354" part="chapter3" ref="N11354" type="section">3.4Pseudomonas-Stämme und Subtypen</cms:entry><cms:entry id="N1135E" part="chapter3" ref="N1135E" type="citenumber">14</cms:entry><cms:entry id="N11373" part="chapter3" ref="N11373" type="citenumber">15</cms:entry><cms:entry id="N11379" part="chapter3" ref="N11379" type="table"/><cms:entry id="N11413" part="chapter3" ref="N11413" type="mm">605#667</cms:entry><cms:entry id="N1142A" part="chapter3" ref="N1142A" type="citenumber">16</cms:entry><cms:entry id="N11430" part="chapter3" ref="N11430" type="table"/><cms:entry id="N114FA" part="chapter3" ref="N114FA" type="citenumber">17</cms:entry><cms:entry id="N114FD" part="chapter3" ref="N114FD" type="mm">605#679</cms:entry><cms:entry id="chapter4" part="chapter4" ref="chapter4" type="chapter">4 Diskussion</cms:entry><cms:entry id="N11519" part="chapter4" ref="N11519" type="helpercitenumber">17</cms:entry><cms:entry id="N11522" part="chapter4" ref="N11522" type="section">4.1 Diagnostische Wertigkeit von mikrobiologischen Kulturen</cms:entry><cms:entry id="N1152F" part="chapter4" ref="N1152F" type="citenumber">18</cms:entry><cms:entry id="N11572" part="chapter4" ref="N11572" type="citenumber">19</cms:entry><cms:entry id="N11588" part="chapter4" ref="N11588" type="section">4.2 Methoden der Genotypisierung</cms:entry><cms:entry id="N1159F" part="chapter4" ref="N1159F" type="citenumber">20</cms:entry><cms:entry id="N115E5" part="chapter4" ref="N115E5" type="section">4.3 Intraindividuelle und longitudinale Variabilität der Pseudomonas-Genotypen</cms:entry><cms:entry id="N115EF" part="chapter4" ref="N115EF" type="citenumber">21</cms:entry><cms:entry id="N11638" part="chapter4" ref="N11638" type="citenumber">22</cms:entry><cms:entry id="chapter5" part="chapter5" ref="chapter5" type="chapter">5 Zusammenfassung</cms:entry><cms:entry id="N11646" part="chapter5" ref="N11646" type="helpercitenumber">22</cms:entry><cms:entry id="N11654" part="chapter5" ref="N11654" type="citenumber">23</cms:entry><cms:entry ref="N1165D" type="back"/><cms:entry id="N1165F" part="N1165F" ref="N1165F" type="abbreviation">Abkürzungsverzeichnis</cms:entry><cms:entry id="N11666" part="N1165F" ref="N11666" type="table"/><cms:entry ref="N117FD" type="bibliography">Literaturverzeichnis</cms:entry><cms:entry ref="I_bib1" type="link"/><cms:entry ref="I_bib2" type="link"/><cms:entry ref="I_bib3" type="link"/><cms:entry ref="I_bib4" type="link"/><cms:entry ref="I_bib5" type="link"/><cms:entry ref="I_bib6" type="link"/><cms:entry ref="I_bib7" type="link"/><cms:entry ref="I_bib8" type="link"/><cms:entry ref="I_bib9" type="link"/><cms:entry ref="I_bib10" type="link"/><cms:entry ref="I_bib11" type="link"/><cms:entry ref="I_bib12" type="link"/><cms:entry ref="I_bib13" type="link"/><cms:entry ref="I_bib14" type="link"/><cms:entry ref="I_bib15" type="link"/><cms:entry ref="I_bib16" type="link"/><cms:entry ref="I_bib17" type="link"/><cms:entry ref="I_bib18" type="link"/><cms:entry ref="I_bib19" type="link"/><cms:entry ref="I_bib20" type="link"/><cms:entry ref="I_bib21" type="link"/><cms:entry ref="I_bib22" type="link"/><cms:entry ref="I_bib23" type="link"/><cms:entry ref="I_bib24" type="link"/><cms:entry ref="I_bib25" type="link"/><cms:entry ref="I_bib26" type="link"/><cms:entry ref="I_bib27" type="link"/><cms:entry ref="I_bib28" type="link"/><cms:entry ref="I_bib29" type="link"/><cms:entry ref="I_bib30" type="link"/><cms:entry ref="I_bib31" type="link"/><cms:entry ref="I_bib32" type="link"/><cms:entry ref="I_bib33" type="link"/><cms:entry ref="I_bib34" type="link"/><cms:entry ref="I_bib35" type="link"/><cms:entry ref="I_bib36" type="link"/><cms:entry ref="I_bib37" type="link"/><cms:entry ref="I_bib38" type="link"/><cms:entry ref="I_bib39" type="link"/><cms:entry ref="I_bib40" type="link"/><cms:entry ref="I_bib41" type="link"/><cms:entry ref="I_bib42" type="link"/><cms:entry ref="I_bib43" type="link"/><cms:entry ref="I_bib44" type="link"/><cms:entry ref="I_bib45" type="link"/><cms:entry ref="I_bib46" type="link"/><cms:entry ref="I_bib47" type="link"/><cms:entry ref="I_bib48" type="link"/><cms:entry ref="I_bib49" type="link"/><cms:entry ref="I_bib50" type="link"/><cms:entry ref="I_bib51" type="link"/><cms:entry ref="I_bib52" type="link"/><cms:entry ref="I_bib53" type="link"/><cms:entry ref="I_bib54" type="link"/><cms:entry ref="I_bib55" type="link"/><cms:entry ref="I_bib56" type="link"/><cms:entry ref="I_bib57" type="link"/><cms:entry ref="I_bib58" type="link"/><cms:entry ref="I_bib59" type="link"/><cms:entry ref="I_bib60" type="link"/><cms:entry ref="I_bib61" type="link"/><cms:entry ref="I_bib62" type="link"/><cms:entry ref="I_bib63" type="link"/><cms:entry ref="I_bib64" type="link"/><cms:entry ref="I_bib65" type="link"/><cms:entry ref="I_bib66" type="link"/><cms:entry ref="I_bib67" type="link"/><cms:entry ref="I_bib68" type="link"/><cms:entry ref="I_bib69" type="link"/><cms:entry ref="I_bib70" type="link"/><cms:entry ref="I_bib71" type="link"/><cms:entry ref="I_bib72" type="link"/><cms:entry ref="I_bib73" type="link"/><cms:entry ref="I_bib74" type="link"/><cms:entry ref="I_bib75" type="link"/><cms:entry ref="I_bib76" type="link"/><cms:entry ref="I_bib77" type="link"/><cms:entry id="N120FC" part="N120FC" ref="N120FC" type="acknowledgement">Danksagung</cms:entry><cms:entry id="N1210E" part="N1210E" ref="N1210E" type="vita">Lebenslauf</cms:entry><cms:entry id="N12115" part="N1210E" ref="N12115" type="table"/><cms:entry id="N1252A" part="N1252A" ref="N1252A" type="appendix">Publikationen</cms:entry><cms:entry id="N1252C" part="N1252A" ref="N1252C" type="head"/><cms:entry id="N1252F" part="N1252A" ref="N1252F" type="p"/><cms:entry id="N12535" part="N1252A" ref="N12535" type="p"/><cms:entry id="N12541" part="N1252A" ref="N12541" type="p"/><cms:entry id="N12547" part="N1252A" ref="N12547" type="p"/><cms:entry id="N12553" part="N1252A" ref="N12553" type="p"/><cms:entry id="N12559" part="N1252A" ref="N12559" type="p"/><cms:entry id="N1256B" part="N1252A" ref="N1256B" type="p"/><cms:entry id="N12571" part="N1252A" ref="N12571" type="p"/><cms:entry id="N12596" part="N12596" ref="N12596" type="declaration">Eidesstattliche Erklärung</cms:entry><cms:entry part="N117FD" type=":current"/><cms:entry type=":lang">de</cms:entry><cms:entry id=":contents" part="front" ref=":contents" type=":contents">Inhaltsverzeichnis</cms:entry><cms:entry type=":help"><url 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