Isolierung und Kryokonservierung von vaskulären Zellen aus menschlichen Nabelschnurgefäßen für das  Tissue Engineering von kardiovaskulären Geweben (Herzklappen)Liv Krämer131.1244#2434605#26751.26Allgemeiner Teil411#4247384#2748910Zellquelle1.311413#38312252#97273#29213342#3882142.1Zellgewinnung 15Gewinnung von Endothelzellen Gewinnung von Myofibroblasten16355#2862.217Kryokonservierung der ZellkulturKryokonservierung der gesamten Nabelschnur2.3Auftauen der Zellkulturen18Auftauen der Nabelschnüre2.4Identifikation mittels ZellmorphologieIdentifikation mittels Immunfluoreszenz19Wachstumsverhalten der Zellkulturen2.520Gerüstmaterial276#301 Klappenherstellung21460#28522489#29223358#222 Zellbesiedlung24413#290 Versuchsaufbau25496#21226589#4872.627Histologie Immunhistochemie28Elektronenmikroskopie Quantifizierung des Kollagen-Gehaltes 29 Strukturelle und funktionelle Untersuchungen von kryokonservierten Zellen und dem besiedelten Herzklappenkonstrukten303313.1ZellgewinnungMorphologie der gewonnen Zellen375#26832379#28333375#279379#283 Identifikation mittels indirekter Immunfluoreszenz34496#40735418#37636524#37937605#416605#420 Wachstumsverhalten der Zellkulturen3839627#28640627#2913.241Gerüstmaterial356#249 Klappenherstellung42605#296 Klappenbesiedlung43491#375 Bioreaktor und dynamische Zellkonditionierung 44504#5553.345Histologie und Immunhistochemie480#29546334#247331#24647331#242332#25048323#238331#248 Elektronenmikroskopie49605#319 Untersuchungen auf Kollagen-Gehalt50516#354 Strukturelle und funktionelle Untersuchungen von kryokonservierten Zellen und dem besiedelten Herzklappenkonstrukten51604#26152604#24245354555657461#48258449#34159261#2496061562 Literaturverzeichnis6364604#854Danksagung Erklärung an Eides Statt deInhaltsverzeichnisHilfe Literaturverzeichnis 1 Gaziano, J. M.: Global Burden of Cardiovascular Disease, in Braunwald, Eugene (Hrsg), Douglas P. Zipes, Peter, Libby: Heart disease: a textbook of cardiovascular medicine 6.Auflage, Philadelphia 2001, S.1-18 2 Carabello BA, Crawford FA Jr. Valvular heart disease. N Engl J Med 1997; 337: 32-41 3 Alvaez J, Murday A 1995 Mitral valve repair . Br.J Hosp Med 53: 221-225 4 Hufnagel CA, Harvey WP, Rabil P, McDermott TF (1952) Surgical correction of aortic insufficiency. Surgery 35: 673-683 5 Harken DE, Soroff HS, Taylor WJ, Lefemine AA, Gupta SK, Lunzer S. Partial and complete protheses in aortic insufficiency. J Thorac Cardiovasc Surg 1960; 40: 744 6 Starr A, Edward ML, McCord CW, Griswold HE. Aortic replacement: clinical experience with a semirigid ball-valve prothesis. Circulation 1963; 27: 779. 7 Korossis SA, Fisher J, Ingham E (2000) Cardiac valve replacement: a bioengineering approach. Biomed Mater Eng 10: 83-124 8 BraunwaldE. 1997, Valvular heart disease. In: Heart disaese 5th edition, Braunwald E ed.

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