<?xml version="1.0" encoding="ISO-8859-1"?><cms:container xmlns:cms="http://edoc.hu-berlin.de/diml/module/cms"><cms:document><cms:meta><cms:entry id="front" part="front" ref="front" type="front"/><cms:entry type="title">Isolierung und Kryokonservierung von vaskulären Zellen aus menschlichen Nabelschnurgefäßen für das  Tissue Engineering von kardiovaskulären Geweben (Herzklappen)</cms:entry><cms:entry type="author">Liv Krämer</cms:entry><cms:entry id="chapter1" part="chapter1" ref="chapter1" type="chapter">1</cms:entry><cms:entry id="N10045" part="chapter1" ref="N10045" type="pagenumber">3</cms:entry><cms:entry id="N10049" part="chapter1" ref="N10049" type="section">1.1</cms:entry><cms:entry id="N10079" part="chapter1" ref="N10079" type="mm">244#243</cms:entry><cms:entry id="N1008F" part="chapter1" ref="N1008F" type="pagenumber">4</cms:entry><cms:entry id="N100A2" part="chapter1" ref="N100A2" type="mm">605#267</cms:entry><cms:entry id="N100CF" part="chapter1" ref="N100CF" type="pagenumber">5</cms:entry><cms:entry id="N100E1" part="chapter1" ref="N100E1" type="section">1.2</cms:entry><cms:entry id="N100E4" part="chapter1" ref="N100E4" type="pagenumber">6</cms:entry><cms:entry id="N100E8" part="chapter1" ref="N100E8" type="subsection">Allgemeiner Teil</cms:entry><cms:entry id="N100FA" part="chapter1" ref="N100FA" type="mm">411#424</cms:entry><cms:entry id="N1010E" part="chapter1" ref="N1010E" type="pagenumber">7</cms:entry><cms:entry id="N1012A" part="chapter1" ref="N1012A" type="mm">384#274</cms:entry><cms:entry id="N1013C" part="chapter1" ref="N1013C" type="pagenumber">8</cms:entry><cms:entry id="N10161" part="chapter1" ref="N10161" type="pagenumber">9</cms:entry><cms:entry id="N1018F" part="chapter1" ref="N1018F" type="pagenumber">10</cms:entry><cms:entry id="N10193" part="chapter1" ref="N10193" type="subsection">Zellquelle</cms:entry><cms:entry id="N101B7" part="chapter1" ref="N101B7" type="section">1.3</cms:entry><cms:entry id="N101BA" part="chapter1" ref="N101BA" type="pagenumber">11</cms:entry><cms:entry id="N101CA" part="chapter1" ref="N101CA" type="mm">413#383</cms:entry><cms:entry id="N101DC" part="chapter1" ref="N101DC" type="pagenumber">12</cms:entry><cms:entry id="N101E9" part="chapter1" ref="N101E9" type="mm">252#97</cms:entry><cms:entry id="N101FF" part="chapter1" ref="N101FF" type="mm">273#292</cms:entry><cms:entry id="N1020F" part="chapter1" ref="N1020F" type="pagenumber">13</cms:entry><cms:entry id="N1021B" part="chapter1" ref="N1021B" type="mm">342#388</cms:entry><cms:entry id="chapter2" part="chapter2" ref="chapter2" type="chapter">2</cms:entry><cms:entry id="N1022E" part="chapter2" ref="N1022E" type="pagenumber">14</cms:entry><cms:entry id="N10232" part="chapter2" ref="N10232" type="section">2.1</cms:entry><cms:entry id="N10238" part="chapter2" ref="N10238" type="subsection">Zellgewinnung </cms:entry><cms:entry id="N10250" part="chapter2" ref="N10250" type="pagenumber">15</cms:entry><cms:entry id="N1026A" part="chapter2" ref="N1026A" type="subsection">Gewinnung von Endothelzellen </cms:entry><cms:entry id="N10273" part="chapter2" ref="N10273" type="subsection">
         Gewinnung von Myofibroblasten</cms:entry><cms:entry id="N10276" part="chapter2" ref="N10276" type="pagenumber">16</cms:entry><cms:entry id="N10280" part="chapter2" ref="N10280" type="mm">355#286</cms:entry><cms:entry id="N10293" part="chapter2" ref="N10293" type="section">2.2</cms:entry><cms:entry id="N10296" part="chapter2" ref="N10296" type="pagenumber">17</cms:entry><cms:entry id="N1029A" part="chapter2" ref="N1029A" type="subsection">Kryokonservierung der Zellkultur</cms:entry><cms:entry id="N102A9" part="chapter2" ref="N102A9" type="subsection">Kryokonservierung der gesamten Nabelschnur</cms:entry><cms:entry id="N102AF" part="chapter2" ref="N102AF" type="section">2.3</cms:entry><cms:entry id="N102B2" part="chapter2" ref="N102B2" type="subsection">Auftauen der Zellkulturen</cms:entry><cms:entry id="N102B8" part="chapter2" ref="N102B8" type="pagenumber">18</cms:entry><cms:entry id="N102BF" part="chapter2" ref="N102BF" type="subsection">Auftauen der Nabelschnüre</cms:entry><cms:entry id="N102C5" part="chapter2" ref="N102C5" type="section">2.4</cms:entry><cms:entry id="N102C8" part="chapter2" ref="N102C8" type="subsection">Identifikation mittels Zellmorphologie</cms:entry><cms:entry id="N102CE" part="chapter2" ref="N102CE" type="subsection">Identifikation mittels Immunfluoreszenz</cms:entry><cms:entry id="N102E0" part="chapter2" ref="N102E0" type="pagenumber">19</cms:entry><cms:entry id="N102E7" part="chapter2" ref="N102E7" type="subsection">Wachstumsverhalten der Zellkulturen</cms:entry><cms:entry id="N102FF" part="chapter2" ref="N102FF" type="section">2.5</cms:entry><cms:entry id="N10302" part="chapter2" ref="N10302" type="pagenumber">20</cms:entry><cms:entry id="N10306" part="chapter2" ref="N10306" type="subsection">Gerüstmaterial</cms:entry><cms:entry id="N10318" part="chapter2" ref="N10318" type="mm">276#301</cms:entry><cms:entry id="N1032A" part="chapter2" ref="N1032A" type="subsection">
         Klappenherstellung</cms:entry><cms:entry id="N1032D" part="chapter2" ref="N1032D" type="pagenumber">21</cms:entry><cms:entry id="N10337" part="chapter2" ref="N10337" type="mm">460#285</cms:entry><cms:entry id="N10349" part="chapter2" ref="N10349" type="pagenumber">22</cms:entry><cms:entry id="N1034D" part="chapter2" ref="N1034D" type="mm">489#292</cms:entry><cms:entry id="N10361" part="chapter2" ref="N10361" type="pagenumber">23</cms:entry><cms:entry id="N10368" part="chapter2" ref="N10368" type="mm">358#222</cms:entry><cms:entry id="N1037D" part="chapter2" ref="N1037D" type="subsection">
         Zellbesiedlung</cms:entry><cms:entry id="N10380" part="chapter2" ref="N10380" type="pagenumber">24</cms:entry><cms:entry id="N10396" part="chapter2" ref="N10396" type="mm">413#290</cms:entry><cms:entry id="N103A3" part="chapter2" ref="N103A3" type="subsection">
         Versuchsaufbau</cms:entry><cms:entry id="N103A6" part="chapter2" ref="N103A6" type="pagenumber">25</cms:entry><cms:entry id="N103C2" part="chapter2" ref="N103C2" type="mm">496#212</cms:entry><cms:entry id="N103D0" part="chapter2" ref="N103D0" type="pagenumber">26</cms:entry><cms:entry id="N103D4" part="chapter2" ref="N103D4" type="mm">589#487</cms:entry><cms:entry id="N103E9" part="chapter2" ref="N103E9" type="section">2.6</cms:entry><cms:entry id="N103EC" part="chapter2" ref="N103EC" type="pagenumber">27</cms:entry><cms:entry id="N103F3" part="chapter2" ref="N103F3" type="subsection">Histologie </cms:entry><cms:entry id="N103F9" part="chapter2" ref="N103F9" type="subsection">Immunhistochemie</cms:entry><cms:entry id="N1043B" part="chapter2" ref="N1043B" type="pagenumber">28</cms:entry><cms:entry id="N10454" part="chapter2" ref="N10454" type="subsection">Elektronenmikroskopie</cms:entry><cms:entry id="N1045D" part="chapter2" ref="N1045D" type="subsection">
         Quantifizierung des Kollagen-Gehaltes </cms:entry><cms:entry id="N10460" part="chapter2" ref="N10460" type="pagenumber">29</cms:entry><cms:entry id="N10476" part="chapter2" ref="N10476" type="subsection">
         Strukturelle und funktionelle Untersuchungen von kryokonservierten Zellen und dem besiedelten Herzklappenkonstrukten</cms:entry><cms:entry id="N10479" part="chapter2" ref="N10479" type="pagenumber">30</cms:entry><cms:entry id="chapter3" part="chapter3" ref="chapter3" type="chapter">3</cms:entry><cms:entry id="N104A1" part="chapter3" ref="N104A1" type="pagenumber">31</cms:entry><cms:entry id="N104A5" part="chapter3" ref="N104A5" type="section">3.1</cms:entry><cms:entry id="N104A8" part="chapter3" ref="N104A8" type="subsection">Zellgewinnung</cms:entry><cms:entry id="N104AE" part="chapter3" ref="N104AE" type="subsection">Morphologie der gewonnen Zellen</cms:entry><cms:entry id="N104BA" part="chapter3" ref="N104BA" type="mm">375#268</cms:entry><cms:entry id="N104CA" part="chapter3" ref="N104CA" type="pagenumber">32</cms:entry><cms:entry id="N104D4" part="chapter3" ref="N104D4" type="mm">379#283</cms:entry><cms:entry id="N104E4" part="chapter3" ref="N104E4" type="pagenumber">33</cms:entry><cms:entry id="N104EB" part="chapter3" ref="N104EB" type="mm">375#279</cms:entry><cms:entry id="N104FE" part="chapter3" ref="N104FE" type="mm">379#283</cms:entry><cms:entry id="N1050B" part="chapter3" ref="N1050B" type="subsection">
         Identifikation mittels indirekter Immunfluoreszenz</cms:entry><cms:entry id="N1050E" part="chapter3" ref="N1050E" type="pagenumber">34</cms:entry><cms:entry id="N1051B" part="chapter3" ref="N1051B" type="mm">496#407</cms:entry><cms:entry id="N1052D" part="chapter3" ref="N1052D" type="pagenumber">35</cms:entry><cms:entry id="N10531" part="chapter3" ref="N10531" type="mm">418#376</cms:entry><cms:entry id="N10541" part="chapter3" ref="N10541" type="pagenumber">36</cms:entry><cms:entry id="N10548" part="chapter3" ref="N10548" type="mm">524#379</cms:entry><cms:entry id="N1055A" part="chapter3" ref="N1055A" type="pagenumber">37</cms:entry><cms:entry id="N1055E" part="chapter3" ref="N1055E" type="mm">605#416</cms:entry><cms:entry id="N1056E" part="chapter3" ref="N1056E" type="mm">605#420</cms:entry><cms:entry id="N10579" part="chapter3" ref="N10579" type="subsection">
         Wachstumsverhalten der Zellkulturen</cms:entry><cms:entry id="N1057C" part="chapter3" ref="N1057C" type="pagenumber">38</cms:entry><cms:entry id="N105A7" part="chapter3" ref="N105A7" type="pagenumber">39</cms:entry><cms:entry id="N105B1" part="chapter3" ref="N105B1" type="mm">627#286</cms:entry><cms:entry id="N105C6" part="chapter3" ref="N105C6" type="pagenumber">40</cms:entry><cms:entry id="N105D3" part="chapter3" ref="N105D3" type="mm">627#291</cms:entry><cms:entry id="N105E5" part="chapter3" ref="N105E5" type="section">3.2</cms:entry><cms:entry id="N105E8" part="chapter3" ref="N105E8" type="pagenumber">41</cms:entry><cms:entry id="N105EC" part="chapter3" ref="N105EC" type="subsection">Gerüstmaterial</cms:entry><cms:entry id="N105F5" part="chapter3" ref="N105F5" type="mm">356#249</cms:entry><cms:entry id="N1060B" part="chapter3" ref="N1060B" type="subsection">
         Klappenherstellung</cms:entry><cms:entry id="N1060E" part="chapter3" ref="N1060E" type="pagenumber">42</cms:entry><cms:entry id="N10618" part="chapter3" ref="N10618" type="mm">605#296</cms:entry><cms:entry id="N10630" part="chapter3" ref="N10630" type="subsection">
         Klappenbesiedlung</cms:entry><cms:entry id="N10633" part="chapter3" ref="N10633" type="pagenumber">43</cms:entry><cms:entry id="N10640" part="chapter3" ref="N10640" type="mm">491#375</cms:entry><cms:entry id="N1064D" part="chapter3" ref="N1064D" type="subsection">
         Bioreaktor und dynamische Zellkonditionierung </cms:entry><cms:entry id="N10650" part="chapter3" ref="N10650" type="pagenumber">44</cms:entry><cms:entry id="N1065D" part="chapter3" ref="N1065D" type="mm">504#555</cms:entry><cms:entry id="N1066A" part="chapter3" ref="N1066A" type="section">3.3</cms:entry><cms:entry id="N1066D" part="chapter3" ref="N1066D" type="pagenumber">45</cms:entry><cms:entry id="N10674" part="chapter3" ref="N10674" type="subsection">Histologie und Immunhistochemie</cms:entry><cms:entry id="N10680" part="chapter3" ref="N10680" type="mm">480#295</cms:entry><cms:entry id="N10695" part="chapter3" ref="N10695" type="pagenumber">46</cms:entry><cms:entry id="N1069C" part="chapter3" ref="N1069C" type="mm">334#247</cms:entry><cms:entry id="N106AE" part="chapter3" ref="N106AE" type="mm">331#246</cms:entry><cms:entry id="N106C0" part="chapter3" ref="N106C0" type="pagenumber">47</cms:entry><cms:entry id="N106C4" part="chapter3" ref="N106C4" type="mm">331#242</cms:entry><cms:entry id="N106D6" part="chapter3" ref="N106D6" type="mm">332#250</cms:entry><cms:entry id="N106E8" part="chapter3" ref="N106E8" type="pagenumber">48</cms:entry><cms:entry id="N106EC" part="chapter3" ref="N106EC" type="mm">323#238</cms:entry><cms:entry id="N106FE" part="chapter3" ref="N106FE" type="mm">331#248</cms:entry><cms:entry id="N1070F" part="chapter3" ref="N1070F" type="subsection">
         Elektronenmikroskopie</cms:entry><cms:entry id="N10712" part="chapter3" ref="N10712" type="pagenumber">49</cms:entry><cms:entry id="N10725" part="chapter3" ref="N10725" type="mm">605#319</cms:entry><cms:entry id="N10737" part="chapter3" ref="N10737" type="subsection">
         Untersuchungen auf Kollagen-Gehalt</cms:entry><cms:entry id="N1073A" part="chapter3" ref="N1073A" type="pagenumber">50</cms:entry><cms:entry id="N10744" part="chapter3" ref="N10744" type="mm">516#354</cms:entry><cms:entry id="N10751" part="chapter3" ref="N10751" type="subsection">
         Strukturelle und funktionelle Untersuchungen von kryokonservierten Zellen und dem besiedelten Herzklappenkonstrukten</cms:entry><cms:entry id="N10754" part="chapter3" ref="N10754" type="pagenumber">51</cms:entry><cms:entry id="N1076D" part="chapter3" ref="N1076D" type="mm">604#261</cms:entry><cms:entry id="N1077D" part="chapter3" ref="N1077D" type="pagenumber">52</cms:entry><cms:entry id="N10781" part="chapter3" ref="N10781" type="mm">604#242</cms:entry><cms:entry id="chapter4" part="chapter4" ref="chapter4" type="chapter">4</cms:entry><cms:entry id="N10797" part="chapter4" ref="N10797" type="pagenumber">53</cms:entry><cms:entry id="N107BC" part="chapter4" ref="N107BC" type="pagenumber">54</cms:entry><cms:entry id="N107D2" part="chapter4" ref="N107D2" type="pagenumber">55</cms:entry><cms:entry id="N107F7" part="chapter4" ref="N107F7" type="pagenumber">56</cms:entry><cms:entry id="N10819" part="chapter4" ref="N10819" type="pagenumber">57</cms:entry><cms:entry id="N10823" part="chapter4" ref="N10823" type="mm">461#482</cms:entry><cms:entry id="N10833" part="chapter4" ref="N10833" type="pagenumber">58</cms:entry><cms:entry id="N1083A" part="chapter4" ref="N1083A" type="mm">449#341</cms:entry><cms:entry id="N10859" part="chapter4" ref="N10859" type="pagenumber">59</cms:entry><cms:entry id="N10869" part="chapter4" ref="N10869" type="mm">261#249</cms:entry><cms:entry id="N1087C" part="chapter4" ref="N1087C" type="pagenumber">60</cms:entry><cms:entry id="N1089E" part="chapter4" ref="N1089E" type="pagenumber">61</cms:entry><cms:entry id="chapter5" part="chapter5" ref="chapter5" type="chapter">5</cms:entry><cms:entry id="N108B4" part="chapter5" ref="N108B4" type="pagenumber">62</cms:entry><cms:entry ref="N108BF" type="back"/><cms:entry id="N108C1" part="N108C1" ref="N108C1" type="bibliography">
            Literaturverzeichnis</cms:entry><cms:entry id="N108C5" part="N108C1" ref="N108C5" type="pagenumber">63</cms:entry><cms:entry id="N109FB" part="N108C1" ref="N109FB" type="pagenumber">64</cms:entry><cms:entry id="N110A4" part="N108C1" ref="N110A4" type="mm">604#854</cms:entry><cms:entry ref="N110AA" type="acknowledgement">Danksagung</cms:entry><cms:entry id="N110C2" part="N110C2" ref="N110C2" type="declaration">
            Erklärung an Eides Statt
         </cms:entry><cms:entry part="N110AA" type=":current"/><cms:entry type=":lang">de</cms:entry><cms:entry id=":contents" part="front" ref=":contents" type=":contents">Inhaltsverzeichnis</cms:entry><cms:entry type=":help"><url href="http://...">Hilfe</url></cms:entry></cms:meta><cms:content><acknowledgement id="N110AA">
         <head>Danksagung</head>
         <p>Bei dieser Arbeit haben mich viele Menschen unterstützt, denen ich hiermit herzlichst danken möchte.</p>
         <p>Insbesondere gebührt mein Dank meinem Doktorvater Herrn Professor Dr.med. Dr.hc.mult. Hetzer für die Überlassung des Themas, für seine Unterstützung und Hilfe sowie für die schöne Zeit in seinem Institut. </p>
         <p>Ich danke meinem Betreuer Herrn Dr.med. Sodian, der in mir das Interesse am Gebiet des Tissue Engineering weckte und mir die Möglichkeit zur Erarbeitung dieses Projektes gab. Durch ihn habe ich eine Menge lernen können. </p>
         <p>Ebenso gilt mein Dank Frau Dr. Lüders für ihre besondere Unterstützung. </p>
         <p>Danken möchte ich auch Herrn Professor Kübler, Herrn Professor Kuppe, Professor Meyer und seinem Team sowie Professor Shakibaei für die sehr gute Zusammenarbeit. </p>
         <p>Besonderer Dank gilt all meinen Freunden, die mich immer unterstützen, Stefan Röschert, der mir immer mit Rat zur Seite stand und insbesondere meinen geliebten Eltern, die immer an mich geglaubt haben. </p>
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