List of Abbreviations

A

Adenine

A (Ala)

Alanine

AD

Activation domain

AD fusion library

A cDNA library, constructed in an AD vector such that the protein encoded by the inserts are fused to the AD

AD library plasmid

Plasmid encoding a fusion of the AD and a library insert

AD protein H-REV107-1

A hybrid protein comprised of the AD fused to the H-REV107-1

APS

Ammonium persulphate

BSA

Bovine serum albumin

C

Cytosine

C (Cys)

Cysteine

D (Asp)

Aspartic acid

DABCO

1,4-Diazabicyclo (2,2,2)-octane

DAPI

4',6'-diamidino-2-phenylindole hydrochloride

dATP

Deoxyadenosine Triphosphate

dCTP

Deoxycytosine Triphosphate

dGTP

Deoxyguanine Triphosphate

DMF

Dimethylformamide

DMSO

Dimethyl Sulfoxide

DNA

Deoxyribonucleic Acid

DNA-BD

DNA – binding domain

DNA-BD vector

Plasmid encoding the LexA protein (including the DNA-BD)

dNTP

Deoxyribonucleoside Triphosphate

DTT

Dithiothreitol

dTTP

Deoxythymine Triphosphate

E (Glu)

Glutamic acid

G

Guanine

G (Gly)

Glycine

H (His)

Histidine

His, or Leu, or Trp, or Ura

Yeast colonies require His, or Leu, or Trp, or Ura in the medium to grow; is auxotrophic for one (or more) of these specific nutrients

I (Ile)

Isoleucine

IPTG

Isopropyl-l-thio-β-D-galactopyranoside

L (Leu)

Leucine

LacZ+

Expressed the lacZ reporter gene; is positive for β-galactosidase activity

LB

Luria-Bertani

Leu+

Expressed the LEU2 reporter gene; does not require Leu in the medium to grow

MBD

Membrane Binding Domain

N (Asn)

Asparagine

OD

Optical Density

PBS

Phosphate Buffered Saline

PCR

Polymerase Chain Reaction

PMSF

Phenyl Methyl – Sulfonyl Fluoride

S (Ser)

Serine

SD induction medium

SD medium containing galactose and raffinose as the carbon source; used to induce expression of AD fusion proteins

SD medium

Minimal Synthetic Dropout Medium

SDS

Sodium Dodecyl Sulphate

T

Thymine

T (Thr)

Threonine

Tan

Annealing Temperature

TBST

Tris-Buffered Saline Tween-20

Tm

Melting Temperature

Ura

Uracil

W (Trp)

Tryptophan

X-gal

5-bromo, 4-chloro-3-indol-β-D-galactopyranisode

Y (Tyr)

Tyrosine


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