Superantigen-like interaction of IVIG with antibody Fab fragments cloned by phage display technologyAwuku Kwabena Osei, DEDICATION11.111.21.2.121.2.21.331.3.11.3.241.3.35542#7996560#2361.3.41.4781.591.6101.71.8112112.1122.22.3132.4142.52.5.1152.5.2162.617182.72.7.12.7.2192.8202.9212.9.12.9.2222.102.11232.122.12.12.12.22.12.2.12.12.2.2242.12.3252.132.142633.13.1.127262#2733.1.228567#26129566#18030522#2573.231566#3323.332333.434529#365542#4203.53536581#4523.637523#3983.738567#171567#139393.83.9403.10566#32041566#372423.1143542#35344.1434.2444.3454.446474.5484.6LIST OF ABBREVIATIONSDECLARATIONLIST OF PUBLICATIONSCURRICULUM VITAE ACKNOWLEDGEMENTCITED LITTERATUREenTable of contentsHelp LIST OF ABBREVIATIONS

bp

base pairs

CD

cluster of differentiation

CDR

complementarity-determining region

cfu

colony forming units

CH1

first constant region of antibody heavy chain

CL

light chain consant region

cp

coat protein

D

diversity gene

DTT

dithiothreitol

EBV

Epstein-Barr Virus

ELISA

enzyme linked immunosorbent assay

Fab

VH/CH1 + VL/CL

Fc

crystallizable fragment of antibody molecule

Fd

VH + CH1 of the antibody molecule

FR

Hc

framework region

heavy chain

HIV

Human immunodeficiency virus

HLA

Human Leukocyte Antigen

HRP

horse radish peroxidase

Ig

immunoglobulin

ITP

idiopathic thrombocytopenic purpura

IU

international units

IVIG

intravenous immunoglobulins

JH

heavy chain joining gene

JLLc

light chain joining gene
light chain

M13

VCSM13 helper phage

PBL

peripheral blood lymphocytes

PEG

polyethylene glycol

RIBS

ribosome binding site

rpm

rounds per minute

SCID

severe combined immune deficiency

SLE

systemic lupus erythematosus

SpA

staphylococcal protein A

TNF

Tumor necrosis factor

tu

transforming units

VH

heavy chain variable region

Vκ

kappa chain variable region

VL

light chain variable region

Vλ

lambda chain variable region

w/v

weight by volume