6 Reagenzienliste

Aqua ad injectabila

Steriles Wasser (Ecotainer, Braun, Melsungen)

CLS-Y

Cell Lysis Solution for yeast, algae und fungi (BIO 101)

Dynabeads

Magnetbeads, aus DNA Direct^TMKit, Prod.Nr. 630.02 (DYNAL)

DES

DNA Elution Solution, ultra-pure water (BIO 101)

DNS-Gewichtsmarker

DNA Molecular Weight Marker VI, 1062590 (ROCHE, Mannheim)

dNTP

ATP, CTP, GTP, TTP à 2,5 mM (BRL)

EDTA

10mM (Sigma, Steinheim, Germany, No. E-5134);

Ethidium bromide

1%, 21251 (SERVA)

Ethanol

70% Ethanol (Merck, Darmstadt, Germany, No. 1.00983)

Gelladepuffer

Gel Loading Solution (SIGMA, G-2526)

Kulturbrühe

Bacto,Saboraud Dextrose Broth, 0382-17-9 (Becton Dickinson, Heidelberg)

LB-Medium

Luria-Bertani-Medium, 950ml Aqua ad injectabila, 10g Tryptone, 5g Yeast Extract, 10g NaCl (DIFCO, Detroit)

MgCl₂

Magnesium-Chlorid 50mM (Invitrogen, Gibco)

MgCl₂

Magnesium-Chlorid 25mM (Sigma, Taufkirchen)

Mineralöl

M-5904 (Sigma, Steinheim)

NaCl

Natrium-Chlorid, 1.06404 (1000) (Merck, Darmstadt)

NaOH

Natronlauge, Pätzchen, 50mM (6498) (Merck, Darmstadt))

N3-Puffer

100mM TRIS/H₃PO₄; 15%Ethanol; 900mM KCl; pH 6,3 (NUCLEOBOND)

N4-Puffer

100mM TRIS/H₃PO₄; 15%Ethanol; 1150mM KCl; pH 6,3 (NUCLEOBOND)

N5-Puffer

100mM TRIS/H₃PO₄; 15%Ethanol; 1000mM KCl; pH 8,5 (NUCLEOBOND)

PCR-Puffer

10mM TRIS-HCl; 500mM KCl, pH 8,3; Konzentration 10x (Perkin Elmer)

pCR 2.1-TOPO vector

10ng/µl plasmid DANN (Invitrogen)

Platinum Taq-Polymerase

10x PCR-Buffer minus Mg, 50mM Magnesium Chloride (GIBCOBRL, No. 10966-034)

Proteinase K

200mg/l (Merck, Darmstadt, Germany, No. 1.24568.0500)

RCLB

Red Cell Lysis Buffer: 10mM TRIS; pH 7,6; 5mM MgCl, 10mM NaCl

ROX

6-Carboxy-X-Rhodamin, 150µM (TIB Molbiol)

S1-Puffer

50mM Tris/HCl, 10mM EDTA, 100µg RNase A/ml, pH 8,0 (NUCLEOBOND)

S2-Puffer

200mM NaOH, 1% SDS (NUCLEOBOND)

S3-Puffer

2.80M Kac; pH 5,1 (NUCLEOBOND)

SAB-Agar

Sabouraud-Glucose-Agar, 2% Glucose (Becton Dickinson)

SDS-Lösung

Dodecylsulfat Natriumsalz, (Merck, Darmstadt, Germany, No. 13760.0100);

SEWS-M

Salt Ethanol Wash, purification reagent (BIO 101)

Taq-Polymerase

5U/µl (Perkin Elmer)

TBE-Puffer

10x TBE-Puffer (Serva, Heidelberg, Germany, No. 42557)

TE-Puffer

pH 8; 10mM TRIS; 1mM EDTA (Invitrogen, Gibco)

TRIS

pH 7,6 (TRIS Hydroxymethylaminomethan, Merck, 1.08382)

Waschpuffer

Washing Buffer, DANN Direct^TMKit, Nr. 63002 (DYNAL)

WCLB

White Cell Lysis Buffer: 10mM TRIS; pH 7,6; 10mM EDTA, 50mM NaCl; 0,2% SDS, 200mg/l Proteinase K

Zymolase

1,3-laminaripentaohydrolase von Arthrobacter luteus, ICN 320921