Samples from 101 to 112 were obtained from the collection of the Royal Tropical Institute, Amsterdam.
* M: male; F: female. ** LN: lymph node; BM: bone marrow
*** VL: visceral leishmaniasis, PKDL: post kala-azar dermal leishmaniasis
*** VL/PKDL: simultaneous VL and PKDL, N.N: not known
**** W: western, E: eastern, C: central, S: southern

Serial number

Identification number

Sex

Age in complete

Years

Site of parasite isolation

Diagnosis

*Tribe

or origin

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

1131

1146

32

33

17

20

44

41

45

47

1161

1152

1150

1129

18C

39

1110

1117

36

31

30

29

28

26

22

19

16

15

13

12

11

10

9

8

7

3

2

19C

13C

9C

60

103

100

76

71

61

102

70

M

F

M

M

M

M

M

M

M

M

F

M

F

F

M

M

M

F

M

M

M

M

M

M

M

M

F

M

M

M

M

F

F

F

M

M

F

M

F

M

M

M

M

M

M

M

M

M

9

7

12

25

10

39

13

17

20

18

9

11

12

11

20

17

10

7

18

14

4

18

28

23

19

11

13

20

6

27

8

11

6

5

18

21

13

24

18

11

7

18

4

17

14

14

23

35

LN, BM

LN, BM

LN

LN, BM

LN

LN, BM

LN

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN

LN

LN

LN

LN, BM

LN

LN

LN, BM

LN

LN

LN, BM

LN

LN, BM

LN

LN

LN, BM

LN

LN

LN, BM

LN, BM

LN

LN

LN, BM

LN

LN

LN

LN

LN

LN

LN

VL

N.N

VL

N. N

VL

VL

VL

N. N

VL

N. N

VL

N .N

VL

VL

N. N

VL

N.N

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

W. Sudan

E. Sudan

W. Sudan

Falata

Eretria

Falata

Falata

Falata

Arab

W. Sudan

Falata

Falata

Falata

Falata

W. Sudan

Arab

W. Sudan

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

Falata

W. Sudan

W. Sudan

Falata

W. Sudan

Falata

Falata

W. Sudan

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

W. Sudan

Falata

Eretria

W. Sudan

Eretria

Falata

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

Falata

S. Sudan

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

90

91

92

93

94

95

96

90

101

18

104

4

1

5

1140

1135

72

1116

6

42

40

13N

46

3N

2N

1149

24

14

25

15C

21

1147

35

5C

16C

79

4C

27

8C

3C

7C

20C

2C

10C

66

86

67

C

E

B

A

D

62

75

63

M

M

M

M

M

M

F

F

M

M

M

M

M

F

M

M

M

M

F

M

M

M

M

M

M

M

M

M

M

M

F

F

M

M

M

F

F

M

M

M

M

M

M

M

M

M

M

M

25

25

22

22

12

21

9

8

29

16

4

13

17

12

28

7

1

5

12

13

35

9

2

8

9

14

18

24

24

18

12

12

18

10

15

20

7

14

17

13

20

13

14

30

25

20

17

31

LN, BM

LN, BM

LN, BM

LN

LN

LN

LN

LN

LN, BM

LN

LN

LN, BM

LN

LN, BM

LN

LN

LN, BM

LN, BM

LN

LN

SKIN, LN, BM

LN

LN

LN

LN

LN, BM

LN

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN, BM

LN

LN, BM

LN

LN

LN

LN, BM

LN

LN

LN

LN, BM

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

N. N

N. N

VL

VL

PKDL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

VL

N. N

C. Sudan

W. Sudan

W. Sudan

Eretria

Falata

W. Sudan

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

W. Sudan

W. Sudan

Falata

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

W. Sudan

W. Sudan

W. Sudan

W. Sudan

W. Sudan

Falata

Falata

Arab

W. Sudan

W. Sudan

Arab

W. Sudan

W. Sudan

Falata

W. Sudan

W. Sudan

Falata

W. Sudan

W. Sudan

Arab

Arab

W. Sudan

Falata

Falata

Falata

W. Sudan

W. Sudan

W. Sudan

Eretria

97

98

99

100

101

102

103

104

105

106

107

108

109

110

111

112

81

97

10N

11N

DON12

DON13

DON14

DON15

DON16

DON17

DON18

DON19

DON20

DON2I

DON22

DON23

M

F

M

F

-

-

-

-

-

-

-

-

-

-

-

-

9

13

20

16

-

-

-

-

-

-

-

-

-

-

-

-

LN, BM

LN, BM

LN, BM

LN, BM

BM

BM

BM

BM

BM

BM

LN

BM

BM

BM

BM

LN

N. N

N. N

N. N

N. N

VL

VL/PKDL

VL

VL

VL

VL

PKDL

PKDL

VL

VL

PKDL

PKDL

Falata

W. Sudan

Falata

W. Sudan

N. N

N. N

N. N

N. N

N. N

N. N

N. N

N. N

N. N

N. N

N. N

N. N

Appendix 1: Demographic and clinical data for112 visceral leishmaniasis patients from eastern part of Sudan around Gedaref State (April 1997-November 1998).

n.d.: not done

Serial number

Identification number

SSCP ITS1 pattern

SSCP ITS2 pattern

SSCP gp63 (Y) pattern

SSCP 114 marker pattern

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

38

39

40

41

42

43

44

45

46

47

48

2C

20C

3C

15C

8C

4C

16C

5C

DON 12

DON 13

DON 14

DON15

DON 16

DON 17

DON 18

DON 19

DON 20

DON 21

DON 22

DON 23

MHOM/SD/75/LV139

MHOM/SD/62/LRC-L61

MHOM/SD/68/1S

MHOM/KE/85/NLB323

MHOM/IN/71/LRC-L51a

MHOM/CN/??/Wangjiel

10C

25

1140

1147

24

1135

104

70

4

1149

3

79

35

27

21

40

1

7C

5

42

1116

14

A

A

A

A

A

A

A

A

A

J

D

A

A

D

D

A

A

A

A

D

A

A

A

K

K

N

A

C

C

D

A

A

A

M

C

C

A

A

B

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

J

A

A

A

A

A

A

A

A

A

A

A

A

A

X

Y

Z

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

C

A

A

C

A

A

A

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

A

n.d.

n.d.

n.d.

n.d.

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

B

A

A

A

A

C

A

A

B

A

A

A

A

A

A

n.d

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

A

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

49

50

51

52

53

54

55

56

57

58

59

60

61

62

63

64

65

66

67

68

69

70

71

72

73

74

75

76

77

78

79

80

81

82

83

84

85

86

87

88

89

72

102

97

D

46N

103

44

10N

100

11N

32

75

36

62

9C

16

E

12

15

10

9

7

C

B

13N

26

!7

86

A

66

67

76

71

61

30

31

29

18

22

13C

39

A

A

A

H

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

E

A

A

A

L

H

H

A

F

G

I

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

A

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Appendix 2: Strains of Leishmania donovani analysed in this study and the results of analyses.