In vitro Charakterisierung autologer Aktivierung und Klonierung von T-Lymphozyten aus psoriatischen PlaquesWiebke Dorothea Urban 21.1.1.71.2.1.2.1.891.2.2.101.2.3.111.2.4.121.3.132.143.153.1.3.1.1.3.1.1.1.3.1.1.2.3.1.2.3.1.2.1.3.1.2.2.163.1.2.3.3.1.2.4.173.1.2.5.3.2.183.2.1.3.2.2.3.2.3.3.2.4.193.2.4.1.3.2.4.2.203.2.5.3.2.6.213.2.6.1.3.2.6.2.223.2.7.3.2.7.1.3.2.7.2.234.244.1.4.1.1.4.2.4.2.1.4.2.2.25559#34426522#3774.3.4.3.1.27577#293459#2964.3.2.282930375#35231640#2574.3.3.32481#3144.4.4.4.1.3334605#6884.4.2.35541#328535#279365.37385.1.39405.2.6.41 ABKÜRZUNGSVERZEICHNIS346 Literatur Quellenverzeichnis42434445464748495051 Publikation53DanksagungdeInhaltsverzeichnisHilfe Literatur
Quellenverzeichnis Abrams JR, Kelley SL, Hayes E, Kikuchi T, Brown Mj, Kang S, Lebwohl MG, Guzzo CA, Jegasothy BV, Linsley PS, Krueger JG: Blockade of T-lymphocyte costimulation with cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (CTLA4Ig) reverses the cellular pathology of psoriatic plaques, including the activation of keratinocytes, dendritic cells, and endothelial cells. J Exp Med , Sept 2000, 192 (5) p681-693

Aoki S, Yaoita H, Kitajima Y : An elevated level of autoantibodies against 48-50-kd keratins in the serum of patients with psoriasis. J Invest Dermatol, 1989, 92 p179-183 Asadullah K, Doecke WD, Reinke P, Sterry, Volk HD: Cytokine determination. Diagnostic significance from the clinical and immunological viewpoint. Dtsch Med Wochenschr (Germany), Nov 14 1997, 122 (46) p1424-1431 Baker BS, Swain AF, Valdimarsson H, Fry L: T-cell subpopulations in the blood and skin of patients with psoriasis. Br J Dermatol (England), Jan 1984, 110 (1) p37-44 Baker BS, Swain AF, Fry L, Valdimarsson H: Epidermal T lymphocytes and HLA-DR expression in psoriasis. Br J Dermatol (England), May 1984, 110 (5), p555-564 Baker BS, Powles AV, Lambert S, Valdimarsson H, Fry L: A prospective study of the Koebner reaction and T lymphocytes in uninvolved psoriatic skin. Acta Derm Venereol, 1988, 68 (5) p430-434 Baker BS, Fry l: The immunology of psoriasis. BR J Dermatol, 1992, 126 (1) p1-9 Baker BS, Brent L, Valdimarsson H, Powles AV, Al-Imara L, Walker M, Fry L: Is epidermal cell proliferation in psoriatic skin grafts on nude mice driven by T-cell derived cytokines? Br J Dermatol (England), Feb 1992, 126 (2) p105-110 Baker BS, Bokth S, Powles A, Garioch JJ, Lewis H, Valdimarsson H, Fry L: Group A streptococcal antigen-specific T lymphocytes in guttate psoriatic lesions. Br J Dermatol (England), May 1993, 128 (5) p493-499 Bata-Csorgo Z, Hammerberg C, Voorhees JJ: Kinetics and regulation of human keratinocyte stem cell growth in short-term primary ex vivo culture. Cooperative growth factors from psoriatic lesional T lymphocytes stimulate proliferation among psoriatric uninvolved, but not normal, stem keratinocytes. J Clin Invest, 1995, 95 p317-327 Bjerke JR, Livden JK, Degre M, Matre R: Interferon in suction blister fluid from psoriatric lesions. Br J Dermatol, 1983, 108 (3) p295-299 Blank C, Brown I, Marks R, Nishimura H, Honjo T, Gajewski TF: Absence of programmed death receptor 1 alters thymic development and enhances generation of CD4/CD8 double-negative TCR-transgenic T cells. J Immunol, Nov 2003, 171 p 4574-4581 Boggiano C, Reixach N, Pinilla C, Blondelle SE: Successful identification of novel agents to control infectious diseases from screening mixture-based peptide combinatorial libraries in complex cell-based bioassays. Biopolymers, 2003, 71 (2) p103-116 Borras E, Martin R, Judkowski V, Shukaliak J, Zhao Y, Rubio-Godoy V, Valmori D, Wilson D, Simon R, Houghten R, Pinilla C: Findings on T cell specifity revealed by synthetic combinatorial libraries. Journal of Immunological Methods, 2002, 267, p79-97 Bos JD, De Rie MA: The pathogenesis of psoriasis: Immunological facts and speculations. Immunol Today (England), Jan 1999, 20 (1) p40-46 Boyman O, Hefti HP, Conrad C, Nickoloff BJ, Suter M, Nestle FO: Spontaneous development of psoriasis in a new animal model shows an essential role for resident T cells and tumor necrosis factor-alpha. J Exp Med, Mar 2004, 199 (5) p731-736 Brown DW, Baker BS, Ovigne JM, Hardman C, Powles AV, Fry L: Skin CD4+ T cells produce interferon-gamma in vitro in response to streptococcal antigens in chronic plaque psoriasis. J Invest Dermatol (United States), Mar 2000, 114 (3) p576-580 Chandy KG, Cahalan MD, Grissmer S: Autoimmune diseases linked to abnormal K+ channel expression in double-negative CD4-CD8- T cells. Eur J Immunol, Apr 1990, 20 (4) p747-751 Chang JC, Smith LR, Froning KJ,Schwabe BJ, Laxer JA, Caralli LL, Kurland HH, Karasek MA, Wilkinson DI, Carlo DJ: CD8+ T cells in psoriatic lesions preferentially use T-cell receptor V beta 3 and/ or V beta 13.1 genes. Proc Natl Acad Sci USA (United States), Sep 27 1994, 91 (20) p9282-9286 Chang JC, Smith LR: Persistence of T-cell clones in psoriatic lesions. Arch Dermatol, 1997, 133 p703-708 Crosbie OM, Costello PJ, O'Farrelly C, Hegarty JE: Changes in peripheral blood double-negative T-lymphocyte (CD3+ CD4- CD8-) populations associated with acute cellular rejection after liver transplantation. Liver Transpl Surg, Mar 1998, 4 p141-145 Devi BS, Van Noordin S, Krausz T, Davies KA: Peripheral blood lymphocytes in SLE--hyperexpression of CD154 on T and B lymphocytes and increased number of double negative T cells. J Autoimmun, Oct 1998, 11 (5) p471-475

Eedy DJ, Burrows D, Bridges JM, Jones FG: Clearance of severe psoriasis after allogenic bone marrow transplantation. BMJ, Apr 1990, 300 (6729) p908 Elder JT ,Henseler T, Christophers E, Voorhees JJ, Nair RP: Of genes and antigenes: The inheritance of psoriasis. J Invest Dermatol, Nov 1994, 103 (5) p150-153 Elder JT, Nair RP, Henseler T, Jenisch S, Stuart P, Chia N, Christophers E, Voorhees JJ: The genetics of psoriasis: the odyssey continues. Arch Dermatol, 2001, Vol137 p1447-1454 Fierlbeck G, Rasser G: Psoriasis induced at the injection site of recombinant interferon gamma. Arch Dermatol, 1988, 124 p1811-1815 Fitzpatrick TB: Photochemotherapy of psoriasis. Acta Derm Venereol Suppl (Stockh), 1984, 112 p29-35 Ford MS, Young KJ, Zhang Z, Ohashi PS, Zhang L: The immune regulatory function of lymphoproliferative double negative T cells in vitro and in vivo. J Exp Med, Jul 2002, 196 (2) p261-267 Friedrich M, Krammig S, Henze M, Docke WD, Sterry W, Asadullah K: Flow cytometric characterization of lesional T cells in psoriasis: intracellular cytokine and surface antigen expression indicates an activated, memory/effector type 1 immunophenotype. Arch Dermatol Res (Germany), Oct 2000, 292 (10) p519-521 Gottlieb AB, Lifshitz B, Fu SM, Staiano-Coico L, Wang CY, Carter DM: Expression of HLA-DR molecules by keratinocytes and presence of Langerhans cells in the dermal iniltrate of active psoriatic plaques. J Exp Med, 1986, 164 p1013-1028

Gottlieb SL, Gilleaudeau P, Johnson R, Estes L, Woodworth TG, Gottlieb AB, Krueger JG: Response of psoriasis to a lymphozyte-selective toxin(DAB389-IL-2) suggests a primary immune, but not keratinozyte, pathogenetic basics. Nature Medicine, 1995, 1 (5) p442-447 Griffiths CE, Voorhees JJ, Nickoloff BJ: Characterization of intercellular adhesion molecule-1 and HLA-DR expression in normal and inflamed skin: Modulation by recombinant gamma interferon and tumor necrosis factor. J Am Acad Dermatol (United States), Apr 1989, 20 (4) p617-629 Gundlach BR, Wiesmüller KH, Junt T, Kienle S, Jung G, Walden P: Determination of T cell epitopes with random peptide libraries. Journal of Immunological Methods, 1996, 192 p149-155 Hales JM, Camp RD: Potent T cell stimulatory material with antigenic properties in stratum corneum of normal human skin. J Invest Dermatol (United States), May 1998, 110 (5) p725-729 Hales JM, Camp RD: T-cell stimulating stratum corneum antigens: characterization by chromatography and electrophoresis indicates limited diversity. J Invest Dermatol (United States), Sep 1999, 113 (3) p355-363 Hamad AR, Srikrishnan A, Mirmonsef P, Broeren CP, June CH, Pardoll D, Schneck JP: Lack of coreceptor allows survival of chronically stimulated double-negative alpha/ beta T cells: Implications for autoimmunity. J Exp Med, May 2001, 193 p1113-1121 Hemmer B, Gran B, Zhao Y, Marques A, Pascal J, Tzou A, Kondo T, Cortese I, Bielekova B, Straus SE, McFarland HF, Houghten R, Simon R, Pinilla C, Martin R: Identification of candidate T-cell epitopes and molecular mimics in chronic Lyme disease. Nature (America), 1999, Nat Med, Dec 1999 , 5 (12) p1375-1382 Horrocks C, Holder JE, Berth-Jones J, Camp R: Antigen-independent expansion of T cells from psoriatic skin lesions: phenotypic characterization and antigen reactivity. Br J Dermatol (England), Sep 1997, 137 (3) p331-338 Kelso A: Th1 and Th2 subsets: Paradigms lost? Immunol Today (England), Aug 1995, 16 (8) p374-379 Krueger JG, Krane JF, Carter DM, Gottlieb AB: Role of growth factors, cytokines, and their receptors in the pathogenesis of psoriasis. J Invest Dermatol (United States), Jun 1990, 94 (6) p135-140 Leung DYM, Travers JB, Giorno R, Norris DA, Skinner R, Aelion J, Kazemi LV, Kim MH, Trumble AE, Kotb M: Evidence for a streptococcal superantigen-driven process in acute gutate psoriasis. J Clin Invest, 1995, 96 p2106-2112 Lewis HM, Baker BS, Bokth S, Powles AV, Garioch JJ, Valdimarsson H, Fry L: Restricted T-cell receptor V beta gene usage in the skin of patients with guttate and chronic plaque psoriasis. Br J Dermatol, Nov 1993, 129 (5) p514-520 Lin WJ, Norris DA, Achziger M, Kotzin BL, Tomkinson B: Oligoclonal expansion of intraepidermal T cells in psoriasis skin lesions. J Invest Dermatol (United States), Dec 2001, 117 (6) p1546-1553 Mallon E, Bunker CB: HIV-associated psoriasis. AIDS Patient Care STDS, Mar 2000, 14 (5), p239-246 Masuda T, Ohteki T, Abo T, Seki S, Nose S, Nagura H, Kumagai K: Expansion of the population of double negative CD4-8- T alpha beta-cells in the liver is a common feature of autoimmune mice. J Immunol, Nov 1991, 147 p2907-2912 Menssen A, Trommler P, Vollmer S, Schendel D, Albert E, Gurtler L, Riethmuller G, Prinz JC: Evidence for an antigen-specific cellular immune response in skin lesions of patients with psoriasis vulgaris. J Immunol (United States) , Oct 1995, 155 (8) p4078-4083 Michel G, Kemény L, Homey B, Ruzicka T: FK506 in the treatment of inflammatory skin disease: promises and perspectives. Immunol Today, 1996, 17 p106-108 Mueller W, Hermann B: CyclosporinA for Psoriasis. N Engl J Med, 1976, 301 p355 Nickoloff BJ, Wrone-Smith: Injection of Pre-Psoriatic skin with CD4+T-Cells induces psoriasis. Am J Pathol , Jul 1999, 155 (1) p145-158 Nickoloff BJ: Creation of psoriatic plaques: the ultimate tumor suppressor pathway. A new model for an ancient T-cell-mediated skin disease. Viewpoint. J Cutan Pathol (Denmark), Feb 2001, 28 (2) p57-64, Nickoloff BJ: The cytokine network in psoriasis. Arch Dermatol (United States), Jun 1991, 127 (6) p871-884 Nickoloff BJ: The immunologic and genetic basis of psoriasis. Arch Dermatol (United States), Sep 1999, 135 (9) p1104-1110 Norris DA, Travers JB, Leung DY: Lymphocyte activation in the pathogenesis of psoriasis. J Invest Dermatol (United States), Jul 1997, 109 (1) p1-4

Ovigne JM, Baker BS, Brown DW, Powles AV, Fry L: Epidermal CD8+ T cells in chronic plaque psoriasis are Tc1 cells producing heterogeneous levels of interferon-gamma. Exp Dermatol (Denmark), Jun 2001, 10 (3) p168-174 Powles AV, Baker BS, Rutman AJ, McFadden JP, Valdimarsson H, Fry L: Epidermal rupture is the initiating factor for the Koebner response in psoriasis. Acta Derm Venereol (Sweden), 1990, 70 p35-38 Prinz JC, Gross B, Vollmer S, Trommler P, Strobel I, Meurer M, Plewig G: T cell clones from psoriasis skin lesions can promote keratinocyte proliferation in vitro via secreted products. Eur J Immunol, 1994, 24 (3 ) p593-598 Prinz JC: Psoriasis vulgaris-a sterile antibacterial skin reaction mediated by cross-reactive T cells? An immunological view of the pathophysiology of psoriasis. Clin Exp Dermatol (England), Jun 2001, 26 (4) p326-332 Prinz JC: Which T cells cause psoriasis? Clin Exp Dermatol (England), Jul 1999, 24 (4) p291-295 Robinson JH, Kehoe MA: Group A streptococcal M proteins:virulence factors and protective antigens. Immunol Today, 1992, 13 p362-367 Rubio-Godoy V, Dutoit V, Zhao Y, Simon R, Guillaume P, Houghten R, Romero P, Cerottini J, Pinilla C, Valmori D: Positional scanning-synthetic peptide library-based analysis of self- and pathogen-derived peptide cross-reactivity with tumor-reactive Melan-A-specific CTL. J Immunol, Nov 2002, 169 (10) p5696-5707 Schlaak JF, Buslau M, Jochum W, Hermann E, Girndt M, Gallati H, Meyer zum Buschenfelde KH, Fleischer B: T cells involved in psoriasis vulgaris belong to the Th1 subset. J Invest Dermatol (United States), Feb 1994, 102 (2) p145-149 Sospedra M, Pinilla C, Martin R : Use of combinatorial peptide libraries for T-cell epitope mapping. Methods, 2003, 29 (3) p236-247 Szabo SK, Hammerberg C, Yoshida Y, Bata-Csorgo Z, Cooper KD : Identification and quantitation of interferon-gamma producing T cells in psoriatic lesions: localization to both CD4+ and CD8+ subsets. J Invest Dermatol (United States), Dec 1998, 111 (6) p1072-1078 The International Psoriasis Genetics Study : Assessing Linkage to 14 Candidate Susceptibility Loci in a Cohort of 942 Affected Sib Pairs. Am J Hum Genet, 2003, 73 p430-437 Uyemura K, Yamamura M, Fivenson DF, Modlin RL, Nickoloff BJ: The cytokine network in lesional and lesion-free psoriatic skin is characterized by a T-helper type 1 cell-mediated response. J Invest Dermatol (United States), Nov 1993, 101 (5) p701-705 Valdimarsson H, Baker BS, Jonsdottir I, Fry L: Psoriasis: A disease of abnormal keratinocyte proliferation induced by T-lymphocytes superantigens? Immunol Today (England), 1995, 7 (9) p256-259 Valdimarsson H, Sigmundsdottir H, Jonsdottir I: Is psoriasis induced by streptococcal superantigens and maintained by M-protein-specific T cells that cross-react with keratin? Clin Exp Immunol (England), Jan 1997, 107 Suppl 1 p21-24 Vollmer S, Menssen A, Prinz JC: Dominant lesional T cell receptor rearrangements persist in relapsing psoriasis but are absent from nonlesional skin: evidence for a stable antigen-specific pathogenic T cell response in psoriasis vulgaris. J Invest Dermatol (United States), Nov 2001, 117 (5) p1296-1301

Vollmer S, Menssen A, Trommler P. Schendel D, Prinz JC : T lymphocytes derived from skin lesions of patients with psoriasis vulgaris express a novel cytokine pattern that is distinct from that of T helper type 1 and T helper type 2 cells. Eur J Immunol (Germany), Oct 1994, 24 (10) p2377-2382 Weiss G, Shemer A,Trau H: The Koebner phenomenon: Review of the literature. J Eur Acad Dermatol Venereol, May 2002, 16 (3) p241-248 Whyte Hj, Baughman RD: Acute guttate psoriasis and streptococcal infection. Arch Dermatol, 1964, 89 p350-356 Wrone-Smith T, Nickoloff BJ: Dermal injection of immunocytes induces psoriasis. J Clin Invest, Oct 1996, 98 (8) p1878-1887