Nitric oxide-cGMP signal transduction in the injury, matrix expansion and progression of anti-thy1-induced renal disease of the ratYingrui Wang 111.121.1.11.1.231.1.341.1.41.251.2.161.2.21.2.371.31.3.18577#457595#397101.3.2111.3.31.3.4121.4132142.12.1.115162.1.22.22.2.12.2.2172.2.2.12.2.2.22.2.3182.2.3.12.2.3.22.2.4192.2.52.3202.3.12.3.2212.3.3222.42.4.12.4.22.4.2.12.4.2.2232.4.2.32.4.324252.52.5.12.5.22.5.3262.5.42.5.52.5.6272.5.6.1282.5.6.2292.5.6.32.5.6.4302.5.7312.5.7.1322.5.7.22.5.7.3332.5.7.42.5.7.4.12.5.7.4.2342.5.82.5.8.1352.5.8.2362.5.8.337382.6393403.13.1.1408#3093.1.241453#3073.1.3408#3073.23.2.13.2.23.2.343405#3153.2.4405#307646#2613.2.545405#30746646#25847646#2663.3483.3.13.3.2405#3073.3.349645#5763.3.4408#30751645#5393.3.53.3.5.13.3.5.2646#2743.3.5.3426#3073.3.5.454646#2603.4553.4.13.4.256645#2663.4.357628#4043.4.43.4.4.158400#30959489#5513.4.4.260645#7863.4.562645#5223.4.664646#4973.4.765404#31266645#5543.4.8645#286683.4.9645#247404#3384714.14.1.1724.1.2734.1.3744.1.44.2754.2.14.2.2764.34.3.1774.3.2784.44.4.1794.4.2804.581824.64.6.1834.6.2844.6.2.14.6.2.2854.6.2.3864.787588896909192 Reference list93949596979899100101102103 Abbreviations104105106 Acknowledgements107 Erklärung an Eides Statt108enTable of contentsHelp Abbreviations

°C

Degrees Celsius

aGN

Acute glomerulonephritis

APAAP

Alkaline Phosphatase and Anti-Alkaline Phosphatase

ATP

Adenosine triphosphate

cDNA

Complementary DNA

CO 2

Carbon dioxide

cGMP

cyclic guanosine monophosphate

cGS

Chronic glomerulosclerosis

dATP

Deoxyadenosine 5´-triphosphate

dCTP

Deoxycytidine 5´-triphosphate

dGTP

Deoxyguanosine 5´-triphosphate

dTTP

Deoxythymidine 5´-triphosphate

DEA/NO

Diethylamine-NONOate

DEPC

Diethyl Pyrocarbonate

DNA

Deoxyribonucleic acid

DMEM

Dulbecco’s Modified Eagle’s Medium

dsDNA

Double-stranded DNA

ECM

Extracellular matrix

EDTA

Ethylenediaminetetraacetic acid

ELISA

Enzyme-Linked Immunosorbent Assay

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

GTP

Guanosine 5`-triphosphate

HRP

Horseradish peroxidase

H 2 SO 4

Sulphuric acid

HCl

Hydrochloric acid

IBMX

3-Isobutyl-1-methylxanthine

Ig

Immunoglobulin

LPS

Lipopolysaccharide

Min

Minutes

mRNA

Messenger ribonucleic acid

NADPH

Nicotinamide Adenine Dinucleotide Phosphate Reduced

NO

Nitric oxide

NOS

NO synthase

eNOS

Endothelial NOS

iNOS

Inducible NOS

nNOS

Neuronal NOS

OPD

O-Phenylenediamine dihydrochloride

PAI-1

Plasminogen-activator-inhibitor-type 1

PAS

Periodic acid Schiff

PBS

Phosphate-buffered saline

PCR

Polymerase chain reaction

PDE

Phosphodiesterase

PKG

cGMP-dependent protein kinase

rpm

Revolutions per minute

RPMI

Roswell Park Memorial Institute medium

sec

Seconds

sGC

Soluble guanylate cyclase

TBS

Tris-buffered saline

TGF-beta

Transforming growth factor-beta

TNF-alpha

Tumor necrosis factor-alpha