Jing XIE: Establishment of a two-dimensional electrophoresis map of human mitochondrial proteins
[Front page] Chapter: 1. | 2. | 3. | 4. | 5. | 6. [List of Abbreviations] [Supplementary material] [References] [Acknowledgments] [Curriculum Vitae] [Erklärung]
Page: [Table of contents]
[page 64↓]

Supplementary material

On the following pages the 12 subsections of Fig. 4-12 are depicted in greater detail with protein labels. The numbers in brackets refer to the protein number of the list depicted in Tab. 7-1.

Fig. 4-12#1 : Section 1 of mitochondrial the proteome map

[page 65↓]

Fig. 4-12#2 : Section 2 of the mitochondrial proteome map

[page 66↓]

Fig. 4-12#3 : Section 3 of the mitochondrial proteome map

[page 67↓]

Fig. 4-12#4 : Section 4 of the mitochondrial proteome map

[page 68↓]

Fig. 4-12#5 : Section 5 of the mitochondrial proteome map

[page 69↓]

Fig. 4-12#6 : Section 6 of the mitochondrial proteome map

[page 70↓]

Fig. 4-12#7 : Section 7 of the mitochondrial proteome map

[page 71↓]

Fig. 4-12#8 : Section 8 of the mitochondrial proteome map

[page 72↓]

Fig. 4-12#9 : Section 9 of the mitochondrial proteome map

[page 73↓]

Fig. 4-12#10 : Section 10 of the mitochondrial proteome map

[page 74↓]

Fig. 4-12#11 : Section 11 of the mitochondrial proteome map

Fig.4-11_4 : Section 4 of mitochondrial proteome map

[page 75↓]

Fig. 4-12#12 : Section 12 of the mitochondrial proteome map

Tab. 7-1: The identified proteins of my study. Proteins from the mitochondrial fraction of human lymphoblastiod cells were extracted and separated by 2D-electrophoresis. The identification of proteins was carried out with MALDITOF and MALDI-QTOF mass spectrometry. The database search was performed with several search engines such as Mascot, ProFound, and MS-Fit. Information about a specific protein contains the spot number, the full name, the accession ID and SWISS-PROT number, the E.C. and gi numbers, the theoreticalMW and pI values from SWISS-PROT database [MW(S) and pI(S)] and the measuredMW and pI values [MW(m) and pI(m)].The data from the MS analysis i.e. the numbers of matching peptides and the probability of assignment of a random identity are listed here as well.
M: mitochondrial; MM: mitochondrial matrix; MIM: mitochondrial inner membrane; MOM: mitochondrial outer membrane; MIMS: mitochondrial inter membrane space.

Spot Nr.

gene name

Protein name

swiss ID

SWISS_ PROT Nr.

OMIM No.

gi

E.C.

MW(S)

pI (S)

MW(m)

pI(m)

Seq.

matched peptide (total peptide)

sequence coverage %

probability based Mowse score

Function

location

1

HSPA5

BiP protein. (HSP 70kDa protein 5) (glucose regulated protein 78 kDa, precusor) (GRP78 )

GR78_ HUMAN

P11021

138120

6470150

 

70478,57

5,01

59100

5,20

19 - 654

10(17)

17

105

HSP(assembly)

endoplasmic reticulum lumen

2

TUBB5

tubulin, beta 5.

TBBX_ HUMAN

P04350

602662

18088719

 

49630,87

4,81

51200

5,00

 

7(15)

16

67

?

?

3

ATP5B

ATP synthase, beta chain, mitochondrial precursor.

ATPB_ HUMAN

P06576

102910

114549

3.6.3.14

51769,25

5

47900

5,20

48 - 529

28(60)

58

215

Complex V

M

4

 

not identified

               

5

COX5A

cytochrome C oxidase, subunit Va.

COXA_ HUMAN

P20674

603773

18999392

1.9.3.1

12513,23

4,88

13000

4,80

42 - 150

8(45)

28

64

Complex IV

MIM

6

NDUFS1

NADH-ubiquinone dehydrogenase, Fe-S protein 1. (75kDa) (NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor) (Complex I-75kDa) (CI-75kDa) (NADH-coenzyme Q)

NUAM_ HUMAN

P28331

157655

13637608

1.6.5.3 1.6.99.3

77053,44

5,36

59100

5,80

24-727

16(29)

30

147

Complex I

MIM

7

HSPA9B

heat shock 70kDa protein 9B. (mortalin-2) (stress-70 protein, mitochondrial precursor) (75 kDa glucose regulated protein) (GRP 75) (Peptide-binding protein 74) (PBP74) (Mortalin) (MOT)

GR75_ HUMAN

P38646

600548

4758570

 

68759,00

5,44

57900

5,90

47-679

23(44)

36

215

HSP (apoptosis and chaperone)

M

8

HSPA8

heat shock 70kDa protein 8. (HSP73) (heat shock cognate 71 kDa protein)

HS7C_ HUMAN

P11142

600816

5729877

 

70898,09

5,37

56400

5,70

 

14(20)

24

135

HSP

?

9

HSPD1

heat shock 60kDa protein 1,mitochondrial precursor. (chaperonin) (Hsp60) (CPN60) (heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein) (HuCHA60)

CH60_ HUMAN

P10809

118190

14603309

 

57962,86

5,24

51400

5,70

27-573

33(99)

64

228

HSP (mitochondrial protein import and assembly)

MM

10

HSPD1

heat shock 60kDa protein 1,mitochondrial precursor. (chaperonin) (Hsp60) (CPN60) (heat shock protein 60) (HSP-60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein) (HuCHA60)

CH60_ HUMAN

P10809

118190

14603309

 

57962,86

5,24

51400

5,80

27-573

17(53)

36

124

HSP (mitochondrial protein import and assembly)

MM

11

ACTB

actin, beta. (actin, cytoplasmic 1)

ACTB_ HUMAN

P02570

102630

14250401

 

41605,54

5,29

42700

5,60

2 - 375

16(63)

44

92

 

cytoplasmic

12

ACTB

actin, beta. (actin, cytoplasmic 1)

ACTB_ HUMAN

P02570

102630

14250401

 

41605,54

5,29

42900

5,70

2 - 375

9(30)

23

70

 

cytoplasmic

13

SUCLG2

GTP-specific succinyl-CoA synthetase, beta subunit. (Succinyl-CoA ligase [GDP-forming] beta-chain, mitochondrial precursor) (Succinyl-CoA synthetase, betaG chain) (SCS-betaG)

SCB2_ HUMAN

Q96I99

603922

3766199

6.2.1.4

42564,92

5,48

42200

5,80

38-432

6(24)

15

MS-FIT

citric Cycle

M (membrane protein)

14

---

HSPC108

Q9P042

Q9P042

---

6841440

 

37145,40

5,83

41000

5,80

 

6(20)

28

68

HSP

?

15

PDHB

pyruvate dehydrogenase, E1-beta subunit mitochondrial precursor. (PDHE1-B)

ODPB_ HUMAN

P11177

179060

387010

1.2.4.1

35890,39

5,38

35800

5,90

31 - 359

9(29)

30

72

Pyruvate complex

MM

16

PHB

prohibitin

PHB_ HUMAN

P35232

176705

4505773

 

29804,10

5,57

29000

5,80

 

11(43)

58

119

regulation proliferation

cytoplasmic

17

 

not identified

               

18

ATP5H

ATP synthase H+ transporting,mitochondrial F0 complex, subunit d. (ATP synthase D chain, mitochondrial )

ATPQ_ HUMAN

O75947

---

5453559

3.6.3.14

18360,02

5,22

23100

5,50

 

16(39)

77

201

Complex V

M

19

 

not identified

               

20

PRSS15

lon protease-like protein. (Lon protease homolog, mitochondrial precursor) (LONP) (LONHs)

LONM_ HUMAN

P36776

605490

414046

3.4.21.-

102825,51

5,77

67200

6,20

?-959

11(47)

15

70

AA metabolism

MM

21

HMP

inner membrane protein, mitochondrial. (mitofilin) (motor protein)

IMMT_HUMAN

Q16891

---

5803115

 

83626,35

6,08

62000

6,10

758

5(16)

12

ProFound 1.0e+000 (Z1.55)

TIM

MIM

22

HMP

inner membrane protein, mitochondrial. (mitofilin) (motor protein)

IMMT_HUMAN

Q16891

---

5803115

 

83626,35

6,08

61200

6,10

758

17(32)

28

177

TIM

MIM

23

PDIA3

glucose regulated protein, 58kDa. (protein disulfide isomerase A3 precursor ) (disulfide isomerase ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57) (58 kDa glucose regulated protein)

PDA3_ HUMAN

P30101

602046

20127473

5.3.4.1

54265,22

5,61

51000

6,10

25-505

14(47)

26

105

 

endoplasmic reticulum lumen

24

DLST

dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase, mitochondrial precursor. (E2) (E2K).

ODO2_ HUMAN

P36957

126063

1709442

2.3.1.61

41349,64

5,89

48500

6,00

68-453

12(34)

22

73

citric cycle

M

25

UQCRC1

ubiquinol-cytochrome C reductase core protein I. (ubiquinol-cytochrome C reductase complex, core protein I, mitochondrial precursor )

UCR1_ HUMAN

P31930

191328

4507841

1.10.2.2

49101,57

5,43

44300

6,00

35-480

16(54)

27

94

complex III

MIM

26

---

unknown. (protein for MGC:9832) (actin, beta)

Q96E67

Q96E67

---

15277503

 

40220,13

5,55

41400

6,00

360

9(20)

28

100

 

cytoplasmic

27

IDH3A

isocitrate dehydrogenase 3 (NAD+), alpha. (isocitrate dehydrogenase [NAD], subunit alpha, mitochondrial precursor) (isocitric dehydrogenase) (NAD+-specific ICDH)

IDHA_ HUMAN

P50213

601149

5031777

1.1.1.41

36640,21

5,71

38700

6,00

28-366

10(21)

22

94

citric cycle

M

28

ECHS1

mitochondrial short-chain enoly-CoA hydratase 1, precursor. (enoyl-CoA hydratase, mitochondrial precursor) (SCEH) (enoyl-CoA hydratase 1)

ECHM_ HUMAN

P30084

602292

12707570

4.2.1.17

28354,66

5,88

27600

6,00

28-290

15(34)

44

149

ß-oxidation

MM

29

NDUFV2

NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor. (24-kDa subunit of complex I)

NUHM_ HUMAN

P19404

600532

3123721

1.6.5.3 1.6.99.3

23760.31

5,71

24500

6,00

33-249

9(19)

30

95

Complex I

MIM

[page 77↓]

Spot Nr.

gene name

Protein name

swiss ID

SWISS_ PROT Nr.

OMIM No.

gi

E.C.

MW(S)

pI (S)

MW(m)

pI(m)

Seq.

matched peptide (total peptide)

sequence coverage %

probability based Mowse score

Function

location

30

NDUFA5

NADH ubiquinone oxidoreductase 13 kDa-B subunit. (complex I-13kDa-B)(complex I subunit B13)

NUFM_HUMAN

Q16718

601677

2499316

1.6.5.3 1.6.99.3

13327,50

5,77

12100

5,80

   

sequence

Complex I

MIM

31

COX5A

cytochrome C oxidase, subunit Va. (cytochrome c oxidase polypeptide Va, mitochondrial precursor)

COXA_ HUMAN

P20674

603773

18999392

1.9.3.1

12513,23

4,88

11400

6,00

42-150

6(21)

28

69

Complex IV

MIM
                  

32

OGDH

oxoglutarate (alpha-ketoglutarate) dehydrogenase. (lipoamide) (2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor) (alpha-ketoglutarate dehydrogenase)

ODO1_ HUMAN

Q02218

203740

20541592

1.2.4.2

108880,11

6,24

67000

6,50

41-1002

13(17)

16

133

citric cycle

MM

33

TRAP1

heat shock 75kDa protein, mitochondrial precursor. (HSP 75) (tumor necrosis factor type 1 receptor associated protein) (TRAP-1) (TNFR-associated protein 1)

TRAL_ HUMAN

Q12931

606219

2865466

 

80010,86

8,05

57500

6,40

?-704

21(47)

39

180

HSP (chaperone)

M

34

OXCT

succinyl CoA: 3-ketoacid CoA transferase, mitochondrial precursor. (succinyl CoA:3-oxoacid CoA transferase)

SCOT_ HUMAN

P55809

245050

4557817

2.8.3.5

52089,89

6

51300

6,10

40-520

11(19)

25

87

fat metabolism

MM

35

MCCC2

methycrotonoyl-CoA carboxylase 2 (beta) mitochondrial precursor. (non-biotin containing subunit of 3-methylcrotonyl-CoA carboxylase) (biotin carboxylase) (3-methylcrotonyl-CoA carboxylase 2) (MCCase, beta subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase, beta subunit)

MCCB_ HUMAN

Q9HCC0

210210

11545863

6.4.1.4

61333,20

7,58

50000

6,60

?-563

14(42)

26

99

AA metabolism

MM

36

ATP5A1

ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle

ATPA_ HUMAN

P25705

164360

4757810

3.6.1.34

55209,32

8,28

47900

6,60

44-553

21(45)

43

214

complex V

MIM

37

ACADM

medium-chain acyl-CoA dehydrogenase, mitochondrial precursor.

ACDM_ HUMAN

P11310

607008 201450

2392312

1.3.99.3

43642,89

7,02

41800

6,25

26-421

17(43)

41

173

ß-oxidation

MM

38

ECH1

similar to delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial

ECH1_ HUMAN

Q13011

600696

11433007

5.3.3.-

35994,34

6,61

31600

6,00

?-328

12(29)

44

151

ß-oxidation

M

39

ECH1

similar to delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial

ECH1_ HUMAN

Q13011

600696

11433007

5.3.3.-

35994,34

6,61

31600

6,10

?-328

15(49)

41

112

ß-oxidation

M

40

ETFA

chain A, three-dimensional structure of human electron transfer flavoprotein. (electron transfer flavoprotein alpha-subunit, mitochondrial precursor) (alpha-ETF)

ETFA_ HUMAN

P13804

231680

2781202

 

35079,57

8,62

32300

6,20

?-333

10(25)

40

109

ß-oxidation

MM

41

PRDX3

peroxiredoxin 3. (thioredoxin-dependent peroxide reductase, mitochondrial precursor) (antioxidant protein 1) (AOP-1) (MER5 protein homolog) (HBC189) (PRX III)

PDX3_ HUMAN

P30048

604769

14250063

 

21468,45

5,77

25400

6,00

63-256

5(17)

21

MS-Fit ProFound (1.0e+000)

cell protection

M

42

HADH2

hydroxyacyl-CoA dehydrogenase, type II. (type II HADH) (endoplasmic reticulum-associated amyloid beta-peptide binding protein) (short-chain type dehydrogenase/reductase) (XH98G2)

HCD2_ HUMAN

Q99714

300256

4758504

1.1.1.35

26923,08

7,65

25500

6,10

261

11(37)

58

157

in Alzheimer' disease

endoplasmic reticulum lumen

43

MCCC1

methylcrotonyl-CoA carboxylase alpha chain, mitochondrial precursor. (3-methylcrotonyl-CoA carboxylase 1) (MCCase alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase, alpha subunit)

MCCA_ HUMAN

Q96RQ3

210200

13518228

6.4.1.4

75030,63

6,44

59400

7,00

48-725

8(14)

16

82

AA metabolism

MM

44

PDCD8

programmed cell death 8. (apoptosis-inducing factor)

PCD8_ HUMAN

O95831

300169

4757732

1.-.-.-

55699,48

6,86

58200

7,00

103-613

11(29)

25

120

Apoptosis

MIMS

45

DLD

dihydrolipoamide dehydrogenase, mitochondrial precursor

DLDH_ HUMAN

P09622

246900 238331

181575

1.8.1.4

50147,55

6,35

51500

6,90

36-509

7(19)

22

84

fat metabolism

MM

46

---

unknown. (protein for MGC 14085)

Q96I46

Q96I46

---

14043738

 

51905,08

6,44

49800

6,70

468

11(22)

28

118

?

?

47

ATP5A1

ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit. (ATP synthase alpha chain, mitochondrial precursor)

ATPA_ HUMAN

P25705

164360

4757810

3.6.3.14

55209,32

8,28

48100

6,80

44-553

27(39)

50

273

Complex V

MIM

48

GLUD1

chain A, structure of human glutamate dehydrogenase-Apo form. (glutamate dehydrogenase 1, mitochondrial precursor) (GDH)

DHE3_ HUMAN

P00367

138130 606762

4885281

1.4.1.3

56008,68

6,71

49500

7,00

54-558

18(30)

31

165

AA metabolism

MM

49

GLUD1

chain A, structure of human glutamate dehydrogenase-Apo form. (glutamate dehydrogenase 1, mitochondrial precursor) (GDH)

DHE3_ HUMAN

P00367

138130 606762

20151189

1.4.1.3

56008,68

6,71

49800

7,00

54-558

21(41)

33

159

AA metabolism

MM

50

TUFM

Tu translation elongation factor, mitochondrial precursor. (elongation factor Tu, mitochondrial precursor) (P43)

EFTU_ HUMAN

P49411

602389

4507733

 

45045,00

6,31

42700

6,80

44-452

12(23)

26

132

protein synthesis

M

51

TUFM

Tu translation elongation factor, mitochondrial precursor. (elongation factor Tu, mitochondrial precursor) (P43)

EFTU_ HUMAN

P49411

602389

4507733

 

45045,00

6,31

42600

6,90

44-452

23(41)

50

242

protein synthesis

M

52

ACAT1

mitochondrial acetoacetyl-CoA thiolase. (acetyl-CoA acetyltransferase, mitochondrial precursor)

THIL_ HUMAN

P24752

203750

499158

2.3.1.9

41386,02

8,16

41450

6,90

34-427

11(23)

31

109

ß-oxidation

M

53

VDAC1

voltage-dependent anion channel 1. (voltage-dependent anion-selective channel protein 1) (VDAC-1) (hVDAC1) (outer mitochondrial membrane protein porin 1) (plasmalemmal porin) (Porin 31HL) (Porin 31HM)

POR1_ HUMAN

P21796

604492

4507879

 

30641,40

8,63

35300

6,70

282

16(28)

67

226

VDAC

MOM

54

ETFB

electron-transfer-flavoprotein, beta polypeptide. (Beta-ETF)

ETFB_ HUMAN

P38117

130410

4503609

 

27843,61

8,25

29100

6,40

255

7(24)

28

81

ß-oxidation

MM

55

UQCRFS1

ubiquinol-cytochrome C reductase iron-sulfur subunit, mitochondrial precursor. (rieske iron-sulfur polypeptide 1) (rieske iron-sulfur protein) (RISP)

UCRI_ HUMAN

P47985

191327

5174743

1.10.2.2

21616,58

6,3

28300

6,30

79-274

6(24)

27

79

complex III

MIM

56

COX5B

cytochrome c oxidase polypeptide Vb, mitochondrial precursor.

COXB_ HUMAN

P10606

123866

117103

1.9.3.1

10613,04

6,33

14500

6,40

32-129

6(17)

50

ProFound 1.0e+00

complex IV

MIM

[page 78↓]

Spot Nr.

gene name

Protein name

swiss ID

SWISS_ PROT Nr.

OMIM No.

gi

E.C.

MW(S)

pI (S)

MW(m)

pI(m)

Seq.

matched peptide (total peptide)

sequence coverage %

probability based Mowse score

Function

location

57

 

not identified

               

58

HSPE1

chaperonin 10-related protein. (10 kDa heat shock protein, mitochondrial ) (HSP10) (10 kDa chaperonin)

CH10_ HUMAN

Q04984

600141

4028622

 

10800,50

8,91

11700

6,50

101

8(17)

71

150

HSP (mito protein biogenesis)

MM

59

 

not identified

               

60

ACO2

aconitate hydratase, mitochondrial precursor. (aconitate hydratase) (citrate hydrolyase) (aconitase 2) (aconitase)

ACON_ HUMAN

Q99798

100850

5304852

4.2.1.3

82425,78

6,85

64100

7,20

28-780

28(55)

36

229

citric cycle

M
                  

61

SHMT2

mitochondrial serine hydroxymethyltransferase.

GLYM_ HUMAN

P34897

138450

746436

2.1.2.1

52559,86

8,11

50300

7,30

30-504

24(52)

42

187

nucleotide metabolism

M

62

FH

fumarate hydratase, mitochondrial precursor. (fumarase)

FUMH_ HUMAN

P07954

136850 150800 605839 606812

68293

4.2.1.2

50081,55

6,99

46100

7,20

45-510

12(29)

31

125

citric cycle

M

63

UQCRC2

ubiquinol-cytochrome C reductase core protein II, mitochondrial precursor. (complex III, subunit II)

UCR2_ HUMAN

P22695

191329

12653427

1.10.2.2

46811,05

7,74

46100

7,20

15-453

13(41)

35

114

complex III

MIM

64

FDXR

adrenodoxin reductase. (NADPH: adrenodoxin oxidoreductase, mitochondrial precursor) (AR) (Ferredoxin-NADP(+) reductase)

ADRO_ HUMAN

P22570

103270

178209

1.18.1.2

49967,33

7,11

48000

7,30

33-491

17(30)

37

203

fat metabolism

MM

65

ACAA2

acetyl-CoA acyltransferase 2. (mitochondrial 3-oxoacyl-CoA) (3-ketoacyl-CoA thiolase, mitochondrial ) (Beta-ketothiolase) (T1)

THIM_ HUMAN

P42765

604770

12804931

2.3.1.16

42039,29

8,51

47200

7,20

397

18(45)

49

187

ß-oxidation

M

66

UQCRC2

ubiquinol-cytochrome C reductase core protein II, mitochondrial precursor. (complex III, subunit II)

UCR2_ HUMAN

P22695

191329

12653427

1.10.2.2

46811,05

7,74

46200

7,20

15-453

12(35)

32

105

complex III

MIM

67

ACAT1

mitochondrial acetoacetyl-CoA thiolase. (acetyl-CoA acetyltransferase, mitochondrial precursor)

THIL_ HUMAN

P24752

203750

499158

2.3.1.9

41386,02

8,16

42800

7,00

34-427

11(45)

36

114

ß-oxidation

M

67/2

TOM40

probable mitochondrial import receptor, subunit TOM40 homolog. (translocase of outer membrane 40 kDa subunit homolog) (Haymaker protein) (p38.5)

OM40_ HUMAN

O96008

---

5174723

 

37893,10

6,79

43500

7,00

361

8(48)

27

72

TOM

MOM

68

CPO

coprpoprphyrinogen III oxidase, mito precursor. (coproporphyrinogenase) (coprogen oxidase) (COX)

HEM6_ HUMAN

P36551

121300

547615

1.3.3.3

36853,89

7,31

41300

7,00

32-354

12(21)

32

145

heme biosynthesis

M

69

VDAC1

voltage-dependent anion channel 1. (voltage-dependent anion-selective channel protein 1) (VDAC-1) (hVDAC1) (outer mitochondrial membrane protein porin 1) (plasmalemmal porin) (Porin 31HL) (Porin 31HM))

POR1_ HUMAN

P21796

604492

4507879

 

30641,40

8,63

35900

7,00

282

10(21)

43

141

VDAC

MOM

70

AK2

adenylate kinase 2 isoform A, mitochondrial. (adenylate kinase isoenzyme 2, mitochondrial) (ATP-AMP transphosphorylase)

KAD2_ HUMAN

P54819

103020

7524346

2.7.4.3

26346,55

7,85

30300

7,00

238

10(26)

37

135

maintenance and cell growth

MIMS

71

C21ORF33

anti-sigma cross-reacting protein homolog I beta precursor of human. (ES1 protein homolog, mitochondrial precursor) (Protein KNP-I) (GT335 protein)

ES1_ HUMAN

P30042

601659

2134764

 

24016,57

6,63

27200

6,90

42-268

5(31)

43

64

?

M

72

SOD2

superoxide dismutase [Mn], mitochondrial precursor. (chain A,kinetic analysis of product inhibition in human manganese superoxide dismutase)

SODM_ HUMAN

P04179

147460

134665

1.15.1.1

22204,14

6,86

25300

6,70

25-222

9(15)

44

148

cell protection

MM

73

---

cDNA FLJ20420 fis, clone KAT02462. (hypothetical 26.2 kDa protein) (similar to hypothetical protein, FLJ20420)

Q9NX63

Q9NX63

---

8923390

 

26152,36

8,48

28500

7,00

227

6(20)

26

75

function? Skin?

?

74

SSBP1

single-stranded DNA-binding protein, mitochondrial precursor. (Mt-SSB) (MtSSB) (PWP1-interacting protein 17)

SSB_ HUMAN

Q04837

600439

2624694

 

15195,14

8,24

15700

6,70

17-148

10(28)

70

138

nucleotide metabolism

M

75

GRIM19

NADH-ubiquinone oxidoreductase, B16.6 subunit. (complex I-B16.6) (CI-B16.6) (cell death-regulatory protein GRIM19) (CGI-39 protein) (gene associated with retinoic-interferon-induced mortality 19 protein) (GRIM-19) (CDA016)

NB6M_ HUMAN

Q9P0J0

---

16923946

1.6.5.3 1.6.99.3

16567,12

8,24

16600

6,80

143

11(21)

66

166

Complex I

MIM (membrane protein)

76

 

not identified

               

77

 

not identified

               

78

 

not identified

               

79

HADHA

long-chain-fatty-acid beta-oxidation multienzyme complex, alpha chain, mitochondrial precursor. (trifunctional enzyme alpha subunit, mitochondrial precursor [includes: long-chain enoyl-CoA hydratase (EC 4.2.1.17); long chain 3-hydroxyacyl-CoA dehydrogenase(EC 1.1.1.35)]) (TP-alpha) (78 kDa gastrin-binding protein)

ECHA_ HUMAN

P40939

600890

543064

4.2.1.17 1.1.1.35

79009,87

8,98

62100

7,60

37-763

25(37)

34

225

ß-oxidation

MM

80

CS

citrate synthase, mitochondrial precursor.

CISY_ HUMAN

O75390

118950

15302936

4.1.3.7

49000,22

6,98

47050

7,20

28-466

14(21)

22

126

citric cycle

MM

81

IDH2

isocitrate dehydrogenase [NADP] mitochondrial precursor. (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) (ICD-M)

IDHP_ HUMAN

P48735

147650

20141568

1.1.1.42

46614,35

8,32

47100

7,40

40-452

15(23)

35

197

citric cycle

M

82

IDH2

isocitrate dehydrogenase [NADP] mitochondrial precursor. (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) (ICD-M)

IDHP_ HUMAN

P48735

147650

20141568

1.1.1.42

46614.35

8,32

47100

7,00

40-452

15(30)

38

173

citric cycle

M

[page 79↓]

Spot Nr.

gene name

Protein name

swiss ID

SWISS_ PROT Nr.

OMIM No.

gi

E.C.

MW (S)

pI (S)

MW(m)

pI(m)

Seq.

matched peptide (total peptide)

sequence coverage %

probability based Mowse score

Function

location

83

IDH3B

isocitrate dehydrogenase 3 (NAD+) beta, mitochondrial precursor. (isocitric dehydrogenase) (NAD+-specific ICDH)

IDHB_ HUMAN

O43837

604526

5901982

1.1.1.41

38793,79

7,82

46100

7,20

35-385

10(21)

28

95

citric cycle

M

84

BCAT2

branched chain aminotransferase 2, mitochondrial. (branched-chain amino acid aminotransferase, mitochondrial precursor) (BCAT(m)) (placental protein 18) (PP18)

BCAM_ HUMAN

O15382

113530

4502375

2.6.1.42

39915,56

8,46

43900

7,00

352

11(22)

24

108

AA metabolism

M

85

MDH2

malate dehydrogenase 2, NAD, mitochondrial.

MDHM_ HUMAN

P40926

154100

5174541

1.1.1.37

33000,45

8,54

40800

7,00

25-338

8(25)

30

76

citric cycle

MM

86

CRYZ

crystallin, zeta. (quinone oxidoreductase) (NADPH:quinone reductase)

QOR_ HUMAN

Q08257

123691

13236495

1.6.5.5

35206,63

8,56

41900

7,00

329

6(26)

29

70

detoxication

Cytoplasmic

87

MDH2

malate dehydrogenase 2, NAD, mitochondrial.

MDHM_ HUMAN

P40926

154100

5174541

1.1.1.37

33000,45

8,54

42400

7,00

25-338

14(36)

39

139

citric cycle

MM
                  

88

GAPD

glyceraldehyde-3-phosphate dehydrogenase.

G3P2_ HUMAN

P04406

138400

31645

1.2.1.12

35922,02

8,58

43500

7,00

334

10(17)

35

2.43 (1.0e+000)

 

Cytoplasmic

89

GAPD

glyceraldehyde-3-phosphate dehydrogenase.

G3P2_ HUMAN

P04406

138400

7669492

1.2.1.12

35922,02

8,58

44100

7,10

334

11(27)

27

70

 

Cytoplasmic

90

IDH3G

isocitrate dehydrogenase. (isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial precursor) (isocitric dehydrogenase) (NAD+-specific ICDH)

IDHG_ HUMAN

P51553

300089

12804901

1.1.1.41

38814,53

8,47

47100

7,20

40-393

5(21)

17

MS-Fit

citric cycle

M (membrane protein)

91

GOT2

aspartate aminotransferase 2, mitochondrial precursor. (transaminase A) (glutamate oxaloacetate transaminase-2)

AATM_ HUMAN

P00505

138150

12739307

2.6.1.1

44695,28

8,98

43100

7,60

30-430

12(32)

27

97

AA transporter

MM

92

HADHSC

short chain 3-hydroxyacyl-CoA dehydrogenase, mitochondrial precursor. (HCDH) (medium and short chain L-3-hydroxyacyl-CoA dehydrogenase)

HCDH_ HUMAN

Q16836

601609

2078329

1.1.1.35

32822,73

8,38

37700

7,00

13-314

12(24)

50

140

ß-oxidation

MM

93

AK3

adenylate kinase 4, mitochondrial. (adenylate kinase-4) (AK4) (ATP:AMP phosphotransferase)

KAD4_ HUMAN

P27144

103030

8051579

2.7.4.3

25268,00

8,47

32800

7,00

223

11(19)

34

153

nucleotide metabolism

MM

94

 

not identified

               

95

 

not identified

               

96

HBA1

hemoglobin, alpha chain.

HBA_ HUMAN

P01922

141800 141850 141860

996122

 

15126,36

8,73

12700

7,10

141

5(10)

35

64

 

Cytoplasmic

97

 

not identified

               

98

 

not identified

               

99

 

not identified

               

100

 

not identified

               

101

HBB

hemoglobin, beta chain.

HBB_ HUMAN

P02023

141900 603903

122615

 

15867,22

6,81

10200

7,00

146

  

von sequence

 

Cytoplasmic

102

 

not identified

               

103

HBAP or REA

B-cell associated protein. (B-cell receptor associated protein) (D-prohibitin)

Q99623

Q99623

---

6005854

 

33296,38

9,83

35700

8,10

299

14(26)

51

161

 

B-cell

104

 

not identified

               

105

NIPSNAP1

NIPSNAP homolog 1. (4-nitrophenylphosphatase domain and non-neutonal SNAP25-like 1)

NPS1_ HUMAN

Q9BPW8

603249

4505399

 

33309,98

9,35

29900

8,00

284

9(22)

23

86

?

?

106

ATP5O

ATP synthase H+ transporting, mitochondrial F1 complex, o subunit. (oligomycin sensitivity conferring protein) (human ATP synthase OSCP subunit, oligomycin sensitivity conferring protein)

ATPO_ HUMAN

P48047

600828

4502303

3.6.3.14

20875,49

9,81

26100

8,40

24-213

12(34)

47

110

Complex V

MM

107

 

not identified

               

108

 

not identified

               

109

 

not identified

               

110

 

not identified

               

111

 

not identified

               

112

 

not identified

               

113

 

not identified

               

114

ACTB

actin, beta. (actin, cytoplasmic 1) (fragment)

ACTB_ HUMAN

P02570

102630

16359158

 

41605,54

5,29

10200

8,10

2 - 375

  

von sequence

von sequence

Cytoplasmic

115

 

not identified

               

116

 

not identified

               

117

PNPASE

polynucleotide phosphorylase-like protein

Q8TCS8

Q8TCS8

---

20372922

2.7.7.8

85936,59

7,87

60500

6,50

783

14(20)

18

124

nucleotide metabolism

?

118

OXCT

succinyl CoA: 3-ketoacid CoA transferase, mitochondrial precursor. (succinyl CoA:3-oxoacid CoA transferase)

SCOT_ HUMAN

P55809

245050

4557817

2.8.3.5

52089,89

6

51200

6,10

40-520

5(29)

16

MS-Fit

fat metabolism

MM

[page 80↓]

Spot Nr.

gene name

Protein name

swiss ID

SWISS_ PROT Nr.

OMIM No.

gi

E.C.

MW (S)

pI (S)

MW(m)

pI(m)

Seq.

matched peptide+N106

sequence coverage %

probability based Mowse score

Function

location

119

PMPCA or MPPA or KIAA0123

mitochondrial processing peptidase alpha subunit, mitochondrial precursor. (alpha-MPP) (P-55) (HA1523)

MPPA_ HUMAN

Q10713

---

1709089

3.4.24.64

54624,57

5,75

50100

6,30

34-525

7(22)

16

MS-Fit and ProFound 1.0e+000 (Z1,77)

regulation and assembly(Aa metabolism)

MM

120

LAP3 orLAPEP

cytosol aminopeptidase. (leucine aminopeptidase) (LAP) (leucyl aminopeptidase) (proline aminopeptidase) (prolyl aminopeptidase)

AMPL_ HUMAN

P28838

---

12643394

3.4.11.1 3.4.11.5

52640,08

6,29

49400

6,20

488

9(15)

18

101

regulation and assembly(Aa metabolism)

Cytoplasmic

121

ALDH1B1

aldehyde dehydrogenase X, mitochondrial precursor. (ALDH class 2)

DHA5_ HUMAN

P30837

100670

399363

1.2.1.3

55292,03

6,01

48500

6,00

18-517

10(20)

27

105

detoxication of alcohol

MM

122

PMPCB

mitochondrial processing peptidase beta subunit, mitochondrial precursor. (beta-MPP) (P-52)

MPPB_ HUMAN

O75439

603131

4758734

3.4.24.64

49487,50

5,77

44200

6,00

46-489

7(27)

17

63

protein regulation

MM

123

ACADM

medium-chain acyl-CoA dehydrogenase, mitochondrial precursor.

ACDM_ HUMAN

P11310

607008 201450

17440754

1.3.99.3

43642,89

7,02

41700

6,00

26-421

9(13)

20

131

ß-oxidation

MM
                  

124

ACADM

medium-chain acyl-CoA dehydrogenase, mitochondrial precursor.

ACDM_ HUMAN

P11310

607008 201450

17440754

1.3.99.3

43642,89

7,02

41900

6,50

26-421

10(17)

25

121

ß-oxidation

MM

125

MRPS22

mitochondrial ribosomal protein S22. (gibt protein) (chromosome 3 open reading frame 5) (mitochondrial 28S ribosomal protein S22) (S22mt) (MRP-S22) (GK002)

RT22_ HUMAN

P82650

605810

9910244

 

41280,38

7,7

39400

6,10

360

11(27)

23

97

ribosomal protein

M

126

NDUFS3

NADH-ubiquinone oxidoreductase 30 kDa subunit, mitochondrial precursor. (30-kDa subunit of complex I) (Complex I-30KD) (CI-30KD)

NUGM_ HUMAN

O75489

603846

5138999

1.6.5.3 1.6.99.3

26414,92

5,48

26500

5,90

37-264

15(26)

48

222

Complex I

MIM

127

TSFM

elongation factor Ts, mitochondrial precursor. (EF-Ts) (EF-TsMt)

EFTS_HUMAN

P43897

604723

12644268

 

30503,99

6,26

33200

6,30

46-325

3(38)

 

sequence

protein synthesis

M

128

ETFA

chain A, three-dimensional structure of human electron transfer flavoprotein. (electron transfer flavoprotein alpha-subunit, mitochondrial precursor) (alpha-ETF)

ETFA_ HUMAN

P13804

231680

2781202

 

35079,57

8,62

33200

6,40

?-333

6(25)

28

66

ß-oxidation

MM

129

---

hypothetical protein FLJ113428. (cDNA FLJ11342 fis) (clone PLACE10108009)

Q9NUJ1

Q9NUJ1

---

8923001

 

33932,51

8,81

30600

6,60

306

6(20)

20

71

?

?

130

PYCR1

pyrroline-5-carboxylate reductase. (pyrroline-5-carboxylate reductase 1) (P5CR) (P5C reductase)

PROC_ HUMAN

P32322

179035

5902036

1.5.1.2

33374,65

7,18

34700

7,00

319

11(35)

31

84

Proline biosynthesis

M

131

---

similar to hypothetical protein FLJ10709. (hypothetical 72.6 kDa protein)

Q9BUK4

Q9BUK4

---

8922609

 

72600,98

9,35

57500

7,50

586

23(39)

40

258

?

?

132

SQRDL

sulfide:quinone oxidoreductase, mitochondrial precursor

SQRD_HUMAN

Q9Y6N5

---

10864011

1.-.-.-

49960,71

9,18

55700

7,60

?-450

21(43)

47

218

mitochondrial sulfide oxidation

M

133

HADHB

trifunctional enzyme beta subunit, mitochondrial precursor, [Includes: 3-ketoacyl-CoA thiolase; acetyl-CoA acyltransferase; beta-ketothiolase]. (TP-beta)

ECHB_ HUMAN

P55084

143450

4504327

2.3.1.16

47484,90

9,24

53700

7,50

34-474

18(29)

31

154

ß-oxidation

MM

134

AFXN1

sideroflexin 1

SFX1_ HUMAN

Q9H9B4

---

20140250

 

35619,42

9,22

39600

7,30

322

7(17)

20

87

iron transport

M (membrane protein)

135

AKL3L

adenylate kinase-3,mitochondrial. (adenylate kinase 3) (AK3) (AK3-alpha) (GTP:AMP phosphotransferase)

KAD3_ HUMAN

Q9UIJ7

---

11130641

2.7.4.10

25434,22

9,16

30100

7,50

226

10(21)

50

154

nucleotide metabolism

MM

136

GBAS

NIPSNAP2 protein. (glioblastoma amplified sequence)

NPS2_ HUMAN

O75323

603004

2769254

 

33742,64

9,42

30100

7,40

286

6(11)

16

70

?

?

137

NDUFB9

NADH-ubiquinone oxidoreductase, B22 subunit. (Complex I-B22) (CI-B22)

NI2M_ HUMAN

Q9Y6M9

601445

6274550

1.6.5.3 1.6.99.3

21699,72

8,59

28900

7,20

178

10(29)

46

109

complex I

MIM

138

NDUFB10

NADH-ubiquinone oxidoreductase, PDSW subunit. (Complex I-PDSW) (CI-PDSW)

NIDM_ HUMAN

O96000

603843

4758774

1.6.5.3 1.6.99.3

20645,48

8,77

27300

7,80

171

13(21)

47

145

complex I

MIM

139

 

not identified

               

140

PPIF

peptidyl-prolyl cis-trans isomerase, mitochondrial precursor. (PPIase) (rotamase) (peptidyl-prolyl isomerase F) (cyclophilin F)

CYPM_ HUMAN

P30405

604486

5031987

5.2.1.8

18897,38

8,97

20500

8,00

30-207

4(19)

33

sequence

assembly protein

MM

[Front page] [1.] [2.] [3.] [4.] [5.] [6.] [List of Abbreviations] [Supplementary material] [References] [Acknowledgments] [Curriculum Vitae] [Erklärung] [Table of contents]

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