|
Abb
|
Abbildung
|
|
Amp
|
Ampicillin
|
|
AP
|
alkalische Phosphatase
|
|
APC
|
Antigenpräsentierende Zellen (antigen presenting cell)
|
|
APS
|
Ammoniumpersulfat
|
|
bidest
|
entionisiertes Wasser
|
|
BCIP
|
5-Bromo-4-Chloro-3-Indoylphosphat
|
|
bp
|
Basenpaare
|
|
BSA
|
Rinderserumalbumin (bovine serum albumine)
|
|
bzw
|
beziehungsweise
|
|
ca.
|
etwa
|
|
CD
|
Oberflächenantigen (cluster of differentiation)
|
|
cDNA
|
komplementäre DNA
|
|
CFA
|
Freund’s komplettes Adjuvans (complete Freund’s Adjuvant)
|
|
CHO
|
Chinese hamster ovary
|
|
c-Maf
|
a member of the b-ZIP (basic-region leucine-zipper) protein family
|
|
CMV
|
Cytomegalovirus
|
|
COS-7
|
African green monkey kidney
|
|
CTLA-4
|
cytotoxic T lymphocyte antigen 4
|
|
Cy5
|
Indodicarbocyanine
|
|
DC
|
Dendritische Zelle (dendritic cell)
|
|
d.h.
|
das heißt
|
|
DMEM
|
Doulbecco’s MOD EAGLE Medium
|
|
DMSO
|
Dimethylsulfoxid
|
|
DNA
|
Desoxyribonukleinsäure
|
|
dNTP
|
Desoxyribonukleotide
|
|
DTT
|
Dithiothreithol
|
|
EAE
|
experimentell autoimmune Enzephalomyelitis
|
|
E.coli
|
Escherichia coli
|
|
EDTA
|
Ethylendiamintetraessigsäure
|
|
EGFP
|
enhanced green fluorescent protein
|
|
ELISA
|
Enzyme-linked immunoabsorbent assay
|
|
FACS
|
Fluorescence activated cell sorter
|
|
FCS
|
Fetales Kälberserum (fetal calf serum)
|
|
FITC
|
Fluoresceinisocyanat
|
|
FSC
|
forward scatter
|
|
GAD
|
glutamic acid decarboxylase
|
|
GATA-3
|
zinc-finger transcription factor
|
|
GM-CSF
|
Granulozyten-Makrophagen Kolonie-Stimulierender Faktor
|
|
h
|
Stunde (hour)
|
|
HEK
|
human embryonal kidney
|
|
HGPRT
|
Hypoxantin-Guanin-Phosphoribosyl-Transferase
|
|
hTIM3
|
humanes TIM3
|
|
ICOS
|
inducible T-cell co-stimulator
|
|
IDDM
|
insulin dependent diabetes mellitus
|
|
IFA
|
Freund’s inkomplettes Adjuvans (incomplete Freund’s Adjuvant)
|
|
IgG
|
Immunglobulin G
|
|
IL
|
Interleukin
|
|
IPTG
|
Isopropyl β-D-Thiogalaktopyranosid
|
|
IRES
|
internal ribosome entry site
|
|
kDa
|
Kilodalton
|
|
KLH
|
Hämocyanin der Schlüsselloch-Napfschnecke (Keyhole Limpet Heamocyanin)
|
|
LB
|
Luria-Broth
|
|
MBP
|
myelin basic protein
|
|
MCS
|
multiple Klonierungsstelle (multi cloning site)
|
|
MHC
|
Major Histocompatibility Complex
|
|
min
|
Minute
|
|
mRNA
|
messenger RNA
|
|
NBT
|
Nitro Blue Tetrazolium
|
|
NK
|
natürliche Killerzellen
|
|
NOD
|
nonobese diabetic
|
|
NTR
|
nontranslated region
|
|
OD
|
optische Dichte
|
|
ORF
|
offener Leserahmen (open reading frame)
|
|
PBMC
|
Peripheral Blood Mononuclear Cells
|
|
PBS
|
Phosphat-gepufferte Salzlösung (Phosphate buffered saline)
|
|
PCR
|
Polymerasekettenreaktion (Polymerase chain reaction)
|
|
PE
|
Phycoerythrin
|
|
pH
|
pH-Wert
|
|
PLP
|
peptide of myelin proteolipid protein
|
|
PMA
|
Phorbol-12-myristat-13-acetat
|
|
RNA
|
Ribonukleinsäure
|
|
Rnase
|
Ribonuklease
|
|
rpm
|
Umdrehungen pro Minute (rounds per minute)
|
|
RT
|
Raumtemperatur
|
|
RT-PCR
|
Reverse Transkription-Polymerasekettenreaktion
|
|
s.
|
siehe
|
|
SDS
|
Natriumdodecylsulfat
|
|
SDS-PAGE
|
Natriumdodecylsulfat-Polyacrylamid Gelelektrophorese
|
|
sec
|
Sekunden
|
|
SSC
|
sideward scatter
|
|
STAT
|
signal transducer and activator of transcription
|
|
TAE-Puffer
|
Tris-Eisessig-EDTA-Puffer
|
|
Taq
|
Thermus aquarius
|
|
T-bet
|
T-box transcription factor
|
|
TBS
|
Tris gepufferte Kochsalzlösung
|
|
TCR
|
T Zell Rezeptor
|
|
TEMED
|
N,N,N’,N’ Tetramethylendiamin
|
|
TGF-b
|
Transforming Growth Factor b
|
|
Th
|
T Helferzelle (T helper)
|
|
TIM3
|
T-cell immunoglobulin- and mucin-domain-containing molecule 3
|
|
TIM3L
|
Ligand vom TIM3
|
|
TMB
|
3,3’,5,5’Tetramethylbenzidine Dihydrochloride
|
|
TNFa
|
tumor necrosis factor a
|
|
TRIS
|
Tris (hydroxymethyl) aminomethan
|
|
U
|
Unit, Einheit
|
|
usw.
|
und so weiter
|
|
w/v
|
Masse-% vom Gesamtvolumen (weight per volume)
|
|
X-gal
|
5-Brom-4-chlor-3-indolyl-ß-D-galaktopzranosid
|
|
z.B
|
zum Beispiel
|