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Publikationsart: Artikel
Autor(en): Marianne Nagel; Hans-Joachim Schümann
Titel: A Sensitive Method for Determination of Conjugates Catecholamines in Blood Plasma
Erschienen in: Clinical Chemistry and Laboratory Medicine 18 (7) 2009
S. 431-432
Verlag: de Gruyter
ISSN: 1434-6621, 1437-4331
DOI: 10.1515/cclm.1980.18.7.431
Erstveröffentlichung: 1980
Veröffentlichung auf edoc: 01.07.2010
Status: published
peer_reviewed
Volltext: pdf (urn:nbn:de:kobv:11-100172036)
Fachgebiet(e): Chemie
Einrichtung: Kooperation de Gruyter
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Abstract (eng):
Conjugated catecholamines in blood plasma of blood donors were determined from the difference between the total and the free catecholamines. Total catecholamines were measured after complete hydrolysis of the conjugated amines (heating of acidified samples for 40 min at 95°C). Free catecholamines were determined in untreated plasma. Determinations were performed radioenzymatically. We combined and modified the procedures of Peuler & Johnson (1977) Life Sei 27, 625-636) and Da Prada & Z rcher (1976) Life Sei 29, 1161—1174). The resulting method was less laborious and cheaper.
 
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