| Reles, Angela : MOLECULAR GENETIC ALTERATIONS IN OVARIAN CANCER The Role of the p53 Tumor Suppressor Gene and the mdm2 Oncogene |
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aa |
amino acid |
|
Ab |
antibody |
|
APS |
ammoniumpersulfat |
|
Asn |
antisense |
|
bp |
basepair |
|
BPB |
bromophenol blue |
|
BSA |
bovine serum albumine |
|
DEPC |
diethyl-pyrocarbonate |
|
ddH2O |
double destilled H2O |
|
ddATP |
2‘,3‘-dideoxy-adenine-5‘triphosphate |
|
ddCTP |
2‘,3‘-dideoxy-cytosine-5‘triphosphate |
|
ddGTP |
2‘,3‘-dideoxy-guanine-5‘triphosphate |
|
ddTTP |
2‘,3‘-dideoxy-thymine-5‘triphosphate |
|
DNA |
deoxyribonucleic acid |
|
DNA-PK |
DNA-activated protein kinase |
|
dNTP |
2‘-deoxynucleoside-5‘triphosphate |
|
DTT |
dithiotreitol |
|
ECL |
enhanced chemiluminescence |
|
EDTA |
ethylendiamine-tetraacetate |
|
EtBr |
ethidiumbromide |
|
FCS |
fetal calf serum |
|
FIGO |
Fédération Internationale de Gynécologie et d‘Obstétrique |
|
GTB |
glycerol tolerant buffer |
|
HA |
hemagglutinine |
|
HEPES |
(N-[2-hydroxyethyl]piperazine-N‘-[2-ethanesulfonic acid] |
|
HRPO |
horse radish peroxidase |
|
Ig |
immunoglobuline |
|
Im |
immunostaining |
|
kB |
kilo base |
|
kD |
kilo Dalton |
|
LMP |
low malignant potential tumor |
|
LOH |
loss of heterozygosity |
|
mdm2 |
murine double minute 2 gene |
|
MDM2 |
90 kD protein of the mdm2 gene |
|
MOPS |
3-[N-morpholino]propane sulfonic acid |
|
Mut |
Mutation |
|
MW |
molecular weight |
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NER |
nucleotide excision repair |
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NES |
nuclear export signal |
|
OD |
optical density |
|
p53 |
p53 tumor suppressor gene |
|
p53 |
53 kD protein of the p53 gene |
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PAGE |
polyacrylamide gel electrophoresis |
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PCNA |
proliferating cell nuclear antigen |
|
PCR |
Polymerase Chain Reaction |
|
PIG |
p53-induced gene |
|
PMSF |
phenylmethylsulfonylfluoride |
|
RNA |
ribonucleic acid |
|
RT |
room temperature |
|
RT-PCR |
reverse transcriptase polymerase chain reaction |
|
SDS |
sodium dodecyl sulfate |
|
sec |
seconds |
|
Sn |
sense |
|
SF |
serum free |
|
SSCP |
single strand conformation polymorphism |
|
SST |
sequence specific transactivation |
|
TAE |
buffer containing Tris-Acetat and EDTA |
|
TBE |
buffer containing Tris, boric acid, EDTA |
|
TEMED |
N,N,N‘,N‘-tetramethylethylenediamine |
|
UV |
ultra violet |
|
WT |
wild type |
|
XC |
xylenecyanol |
|
X-gal |
5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside |
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