↓24 |
Kit |
Hersteller |
Advantage 2 Polymerase Mix |
Clontech |
QIAquick PCR Purification Kit |
Qiagen |
QIAquick Gel Extraction Kit |
Qiagen |
QIAprep Spin Miniprep Kit |
Qiagen |
QIAprep Spin Maxiprep Kit |
Qiagen |
ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction Kit |
Perkin Elmer |
Quik Change Site-Directed Mutagenesis Kit |
Stratagene |
Polyfect Transfection Reagent |
Qiagen |
TUNEL Enzyme |
Roche |
Oligofectamine |
Invitrogen |
QIAexpressionist |
Qiagen |
SulfoLink |
Pierce |
Quantum RNA 18S Internal Standards |
Ambion |
T4 DNA Ligase |
Promega |
Superscript II Reverse Transkriptase |
Invitrogen |
Vektor |
Hersteller |
pEGFP-C1 |
BD Biosciences Clontech |
pEGFP-N1 |
BD Biosciences Clontech |
pEGFP-N2 |
BD Biosciences Clontech |
pEGFP-N3 |
BD Biosciences Clontech |
pBluescript |
Stratagene |
pCMVTag2A |
Stratagene |
pCMVTag3A |
Stratagene |
GpBUDCE4 |
Invitrogen |
pET32a |
Novagen |
Puffer |
Zusammensetzung |
Bromphenolblau Ladepuffer |
15% Ficoll, 0,25% Bromphenolblau |
Xylencyanol Ladepuffer |
15% Ficoll, 0,25% Xylencyanol |
1xLämmli Puffer |
15g Tris, 72g Glycin, 5g SDS, ad 5l H2O |
50xTAE |
1211,4g Tris, 285ml Eisessig, 93,06g EDTA, ad 5l H2O |
10xPEM |
1M Pipes, 0,05M EGTA, 0,02M MgCl2 |
10xPBS |
1360mM NaCl, 20mM KCl, 106mM Na2HPO4*2H2O, 15mM KH2PO4 |
PBST |
1xPBS, 0,1% Tween20 |
5xBlotting Buffer |
29,1g Tris, 14,65g Glycin, 18,75ml 10% SDS, ad 1l H2O |
2xmagic mix |
48% Harnstoff, 15mM Tris pH7,5, 8,7% Glycerin, 1% SDS, 0,004% Bromphenolblau, 143mM ß-Mercaptoethanol |
Mounting Medium |
90% Glycerin, 0,1M Tris pH8, 2,3% DABCO |
DAPI-Medium |
0,5µg/µl DAPI in Mounting-Medium |
5xSDS-PAGE Puffer |
15% ß-Mercaptoethanol, 15% SDS, 1,5% Bromphenolblau, 50% Glycerin |
Puffer A |
0,32M Sucrose, 4mM Hepes pH 7,4, 1%ß-Mercaptoethanol, Complete Mini Protease Inhibitor |
staining Coomassie |
1g Coomassie, 400ml Methanol, 100ml Essigsäure, 500µl H2O |
destaining Coomassie |
400ml Methanol, 100ml Essigsäure, 500µl H2O |
↓25 |
Substanz |
Hersteller |
Agarose |
Invitrogen |
Sucrose |
Gibco |
Complete Mini Protease Inhibitor |
Roche |
Coomassie Brilliant Blue G 250 |
Serva |
DMEM |
Cambrex |
EMEM |
Cambrex |
DMEM, phosphatefree |
ICN |
FCS |
Biochrom |
Glutamin |
Cambrex |
non essentiel amino acids |
Biochrom |
sodium pyruvate |
SIGMA |
Paraformaldehyd |
Merck |
TritonX100 |
SIGMA |
BSA (bovine serum albumine) |
SIGMA |
Rapamycin |
SIGMA |
Okadaic acid |
SIGMA |
Forskolin |
ICN |
ddForskolin |
ICN |
IBMX (3-Isobutyl-1-methylxanthine) |
ICN |
Fostriecin |
SIGMA |
LLnL (N-Acetyl-Leu-Leu-Norleucinal) / Calpain inhibitor I |
SIGMA |
Lactacystin |
SIGMA |
LiCl |
Merck |
Wortmannin |
Biomol |
CKI-7 (N-(2-aminoethyl)-5-chloro-isoquinoline-8-sulfonamide) |
Seikagaku |
HePC (Hexadecylphosphocholin) |
SIGMA |
U73122 |
Biomol |
U73343 |
Biomol |
Bradford Reagenz |
SIGMA |
dNTP Set |
Fermentas |
APS (Ammonium Persulfat) |
Biorad |
SDS (Sodiumdodecylsulfate) |
SIGMA |
TEMED |
Gibco |
Acrylamid/bis-Acrylamid 37,5:1 |
Roth |
Tris (Tris(hydroxymethyl)-aminomethan) |
Merck |
Western lightning Chemiluminescence Reagent |
Perkin Elmer |
Trypsin |
Cambrex |
Hepes |
Calbiochem |
NaCl |
Merck |
Protein-A-Agarose / Protein-G-Agarose |
Roche |
Ficoll (Type 400) |
Pharmacia |
Antipain-dihydrochloride |
Roche |
Bestatin |
Roche |
Chymostatin |
Roche |
E-64 |
Roche |
Leupeptin |
Roche |
Pepstatin |
Roche |
Phosphoramidon |
Roche |
Pefabloc SC |
Roche |
EDTA-Na2 |
Roche |
Aprotinin |
Roche |
H3 32PO4 in HCl |
Amersham |
Pipes |
SIGMA |
Citrat (tri-Nacitrat-Dihydrat) |
Merck |
Glycin |
Merck |
Triethylamin |
SIGMA |
pd(N)6 |
Pharmacia |
Bromphenolblau |
Serva |
Xylencyanol |
Serva |
Essigsäure (Eisessig) |
Merck |
EDTA |
SIGMA |
EGTA |
SIGMA |
MgCl2 |
Merck |
NaCl |
Merck |
KCl |
Merck |
Na2HPO4 |
Merck |
KH2PO4 |
Merck |
Harnstoff |
Biorad |
Glycerin |
Merck |
ß-Mercaptoethanol |
Merck |
DABCO (1,4-diazobicyclo-2,2,2-octane) |
SIGMA |
DAPI (4,6-Diamidino-2-phenylindole-2HCl*H2O) |
Serva |
DTT |
Promega |
Ethanol |
Merck |
Methanol |
Merck |
Tween 20 |
SIGMA |
DNA-Marker |
Hersteller |
1kb Marker |
Gibco |
Hyperladder I |
Bioline |
Protein-Marker |
Hersteller |
Rainbow Marker |
Amersham |
Kaleidoskop Marker |
Biorad |
Seeblue Marker |
Invitrogen |
Medium |
Zusammensetzung |
LB-Medium |
10 g/l Trypton, 5 g/l Hefeextrakt, 10 g/l NaCl, (15 g/l Agar für Platten), Antibiotikum (Ampicillin oder Kanamycin Konzentration: 50 µg/ml) |
LB low salt Medium |
10 g/l Trypton, 5 g/l Hefeextrakt, 5 g/l NaCl, (15 g/l Agar für Platten), Antibiotikum (Zeocin Konzentration: 50 µg/ml) |
↓26 |
Enzym |
Hersteller |
PP2A |
Upstate |
λ Phosphatase |
NEB |
LAR Tyrosine Phosphatase |
NEB |
Thrombin |
Novagen |
Antikörper |
Hersteller |
anti-V5 Antikörper |
Invitrogen |
anti-c-myc monoclonal Antikörper |
Clontech |
anti-c-myc polyclonal Antikörper |
Santa Cruz |
anti-flag Antikörper |
Stratagene |
anti-tubulin MCA 77s |
Serotec |
anti-tubulin MCA 78s |
Serotec |
anti-ubiquitin |
Santa Cruz |
anti-Aktin |
SIGMA |
anti-PP2A catalytic subunit |
BD Bioscience |
anti-α4 |
Trockenbacher et al., 2001 |
anti-GFP |
Roche |
anti-Lamin A/C |
Chemicon |
anti-GSK3 |
upstate |
Cy3-anti-mouse |
Dianova |
Cy3-anti-rabbit |
Dianova |
Cy3-anti-rat |
Dianova |
FITC-anti-mouse |
Dianova |
FITC-anti-rabbit |
Dianova |
HRP-anti-mouse |
Dianova |
HRP-anti-rabbit |
Amersham |
HRP-anti-rat |
Serotec |
normal mouse IgG |
Santa Cruz Biotechnologie |
Name |
Anwendung |
Version/Internetadresse |
Celera Database |
Homologiesuche |
www.celera.com |
GCG |
Sequenzauswertung / Homologiesuche |
Wisconsin Package Version 10.2 Genetics Computer Group Madison, Wisc. |
STADEN |
Sequenzauswertung |
Version 1999.0 |
NCBI |
Homologiesuche (blast) |
www.ncbi.nlm.nih.gov |
Clustalw |
Sequenzvergleiche (multiple sequence alignment program) | |
prosite |
Vorhersage von Protein-Domänen (database of protein families und domains) |
www.expasy.org/prosite/ |
pfam |
Vorhersage von Protein-Domänen (large collection of multiple sequence alignments and hidden Markov models covering many common protein domains and families) | |
smart |
Vorhersage von Protein-Domänen |
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