Appendix

Abortion rates using high doses of adenoviral vectors :

Table 14 : The application of a higher dose of AdHO-1/GFP did not significantly change abortion rates.

N.P

S.A.

S.A. + 1.10 8 PFU

AdHO-1/GFP

S.A. +

1.10 8 PFU AdEGFP

Mean

2.61

24.73

19.28

22.16

SD

5.42

7.06

10.37

12.86

Median

0

25***

19.4

25

Mean, SD and median of abortion rates. N.P.= Normal Pregnancy; S.A.= Spontaneous abortion.***p ≤ 0.001 when compared to NP by the Mann-Whitney test

Ex-vivo cytokine production as measured by flow cytometry :

Table 15 : ex-vivo cytokine production by decidual lymphocytes

IL-4

IL-10

IFN- γ

TNF- α

N.P.

3.63

2.73

3.96

9.21

S.A.

6.75

7.58

10.47

10.66

S.A. + 1.10 5 AdHO-1/GFP

13.10

2.76

3.83

23.07

S.A. + 1.10 5 AdEGFP

1.00

2.30

4.00

6.38

S.A. + 1.10 8 AdHO-1/GFP

9.69

14.68

17.34

18.65

S.A. + 1.10 8 AdHO-1/GFP

6.05

2.63

2.69

19.89

Cytokine production measured by flow cytometry after PMA/Ionomycin incubation in the presence of monensin. Data are showed as medians. N.P.= Normal Pregnancy; S.A.= Spontaneous abortion.

Table 16 : ex-vivo cytokine production by spleen lymphocytes

IL-4

IL-10

IFN-

TNF-

N.P.

1.15

0.95

1.08

2.28

S.A.

0.65

1.00

1.20

6.54

S.A. + 1.10 5 AdHO-1/GFP

2.57

1.42

1.13

2.57

S.A. + 1.10 5 AdEGFP

0.45

0.38

0.63

1.10

S.A. + 1.10 8 AdHO-1/GFP

0.45

0.63

0.60

6.41

S.A. + 1.10 8 AdHO-1/GFP

1.45

1.57

1.59

2.58

Cytokine production measured by flow cytometry after PMA/Ionomycin incubation in the presence of monensin. Data are showed as medians. N.P.= Normal Pregnancy; S.A.= Spontaneous abortion.

Levels of HO-1 mRNA in the groups receiving high doses of adenovirus :

Table 17 : mRNA levels of HO-1 normalized to β-actin

N.P

S.A.

S.A. + 1.10 8 PFU AdHO-1/GFP

S.A. + 1.10 8 PFU AdEGFP

Mean

0,05076

0,03590

0,06676

0,04263

SD

0,04651

0,01383

0,01629

0,01912

Median

0,02906

0,02967

0,06846*

0,03584

N.P.= Normal Pregnancy; S.A.= Spontaneous abortion. *p≤0,05 as analyzed by the Mann-Whitney Utest for two particular groups. A significant difference was obtained in the group receiving 1.108 PFU AdHO-1/GFP when compared to the S.A. group.


© Die inhaltliche Zusammenstellung und Aufmachung dieser Publikation sowie die elektronische Verarbeitung sind urheberrechtlich geschützt. Jede Verwertung, die nicht ausdrücklich vom Urheberrechtsgesetz zugelassen ist, bedarf der vorherigen Zustimmung. Das gilt insbesondere für die Vervielfältigung, die Bearbeitung und Einspeicherung und Verarbeitung in elektronische Systeme.
DiML DTD Version 4.0Zertifizierter Dokumentenserver
der Humboldt-Universität zu Berlin
HTML generated:
29.09.2014