2013-03-04Zeitschriftenartikel
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
Akerboom, Jasper; Carreras Calderón, Nicole; Tian, Lin; Wabnig, Sebastian; Prigge, Matthias; Tolö, Johan; Gordus, Andrew; Orger, Michael; Severi, Kristen; Macklin, John J.; Patel, Ronak; Pulver, Stefan R.; Wardill, Trevor; Fischer, Elisabeth; Schüler, Christina; Chen, Tsai-Wen; Sarkisyan, Karen S.; Marvin, Jonathan; Bargmann, Cornelia; Kim, Douglas S.; Kügler, Sebastian; Lagnado, Leon; Hegemann, Peter; Gottschalk, Alexander; Schreiter, Eric; Looger, Loren L.
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent ...
