Fast manipulation of cellular cAMP level by light in vivo
Schröder-Lang, Saskia
Schwärzel, Martin
Seifert, Reinhard
Strünker, Timo
Kateriya, Suneel
Looser, Jens
Watanabe, Masakatsu
Watanabe, Masakatsu
Kaupp, U Benjamin
Hegemann, Peter
Nagel, Georg
Mathematisch-Naturwissenschaftliche Fakultät I
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACa and PACb. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
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