2006-11-26Zeitschriftenartikel DOI: 10.1038/NMETH975
Fast manipulation of cellular cAMP level by light in vivo
Mathematisch-Naturwissenschaftliche Fakultät I
The ﬂagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the ﬂavoproteins PACa and PACb. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
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