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2005-04-09Zeitschriftenartikel DOI: doi:10.1016/j.abb.2005.03.020
CYP35: Xenobiotically induced gene expression in the nematode Caenorhabditis elegans
dc.contributor.authorMenzel, Ralph
dc.contributor.authorRödel, Matthias
dc.contributor.authorKulas, Jana
dc.contributor.authorSteinberg, Christian E.W.
dc.date.accessioned2017-06-17T00:56:36Z
dc.date.available2017-06-17T00:56:36Z
dc.date.created2008-05-12
dc.date.issued2005-04-09
dc.date.submitted2005-03-11
dc.identifier.issn0003-9861
dc.identifier.urihttp://edoc.hu-berlin.de/18452/10046
dc.description.abstractAlthough over 80 cytochrome P450 (CYP) encoding genes have been identified in the genome of the nematode Caenorhabditis elegans very little is known about their involvement in biotransformation. This paper demonstrates a concentration-dependent relationship of C. elegans CYP35A1, A2, A5, and C1 gene expression in response to four organic xenobiotics, namely atrazine, PCB52, fluoranthene, and lansoprazole. The toxicity of these xenobiotics was determined using a reproduction assay. CYP-specific messenger RNA expression was analyzed by semi-quantitative RT-PCR resulting in a strongly increasing, concentration-dependent induction well below the EC50 for reproduction. For PCB52, approximately 0.5% of the EC50 induces a 2-fold increase of CYP35 gene expression. Using a double mutant and multiple RNAi of CYP35A/C it was possible to diminish the reproduction decline caused by PCB52 and fluoranthene.eng
dc.language.isoeng
dc.publisherHumboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I
dc.subjectCaenorhabditis eleganseng
dc.subjectCytochrome P450eng
dc.subjectCYP35eng
dc.subjectOrganic xenobioticseng
dc.subjectGene expressioneng
dc.subjectReproduction assayeng
dc.subjectRNAieng
dc.subject.ddc570 Biowissenschaften, Biologie
dc.titleCYP35: Xenobiotically induced gene expression in the nematode Caenorhabditis elegans
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-10088287
dc.identifier.doidoi:10.1016/j.abb.2005.03.020
dc.identifier.doihttp://dx.doi.org/10.18452/9394
local.edoc.container-titleArchives of Biochemistry & Biophysics
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultät I
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.elsevier.com/wps/find/journalabstracting.cws_home/622787/abstracting#abstracting
local.edoc.container-volume483
local.edoc.container-issue1
local.edoc.container-year2005
local.edoc.container-firstpage93
local.edoc.container-lastpage102
dc.description.versionPeer Reviewed

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