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2009-10-14Zeitschriftenartikel DOI: 10.18452/9601
Use of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
dc.contributor.authorBrilha, Sara
dc.contributor.authorProença, Helena
dc.contributor.authorCristino, José Melo
dc.contributor.authorHänscheid, Thomas
dc.date.accessioned2017-06-17T01:36:45Z
dc.date.available2017-06-17T01:36:45Z
dc.date.created2010-07-01
dc.date.issued2009-10-14
dc.identifier.issn1437-4331
dc.identifier.urihttp://edoc.hu-berlin.de/18452/10253
dc.description.abstractBackground: Cultures for urinary tract infections (UTI) constitute a large workload in the clinical microbiology laboratory, although up to 80% are usually negative. Several automated methods are available to screen urines for UTI, one being the flow cytometry-based Sysmex® UF-100. Methods: The performance of the UF-100 was evaluated over a 16-month period using urine culture as the reference method. Results: During this period, a total of 5356 urine samples were studied (469 children; 3229 women and 1658 men), of which 706 were culture positive (593 grew Gram negative bacilli). Receiver operating characteristics (ROC) curve analysis showed an area under the curve (AUC) of 0.83 for leukocytes and 0.85 for bacterial count. Applying cut-off values reported in the literature gave sensitivities ranging from 75% to 90%, resulting in 73–174 false negatives (FN). Using a logical combination (leukocytes ≥15×106/L OR bacteria ≥500×106/L) gave a sensitivity of 98%. However, the specificity dropped to 25%, resulting in 15 FN. Conclusions: Screening urine samples for UTI detects a large number of culture positive samples. However, the rather large number of FN observed precludes the use of the UF-100 as a routine screening method to exclude urine samples from culture. Clin Chem Lab Med 2010;48:289–92.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleUse of flow cytometry (Sysmex® UF-100) to screen for positive urine cultures: in search for the ideal cut-off
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100114530
dc.identifier.doihttp://dx.doi.org/10.18452/9601
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-year2010
dc.description.versionPeer Reviewed
dcterms.bibliographicCitation.doi10.1515/CCLM.2010.047
dcterms.bibliographicCitation.journaltitleClinical Chemistry and Laboratory Medicine
dcterms.bibliographicCitation.volume48
dcterms.bibliographicCitation.issue2
dcterms.bibliographicCitation.originalpublishernamede Gruyter
dcterms.bibliographicCitation.pagestart289
dcterms.bibliographicCitation.pageend292

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