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2009-07-09Zeitschriftenartikel DOI: 10.18452/9938
Pre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
dc.contributor.authorHan, Fei
dc.contributor.authorLv, Rong
dc.contributor.authorJin, Juan
dc.contributor.authorWu, Jianyong
dc.contributor.authorChen, Ying
dc.contributor.authorWang, Huiping
dc.contributor.authorChen, Jianghua
dc.date.accessioned2017-06-17T02:43:21Z
dc.date.available2017-06-17T02:43:21Z
dc.date.created2010-07-01
dc.date.issued2009-07-09
dc.identifier.issn1437-4331
dc.identifier.urihttp://edoc.hu-berlin.de/18452/10590
dc.description.abstractBackground: Endothelial cell antigens are important targets in acute rejection (AR). Our goal was to measure the serum concentrations of pre-transplant anti-endothelial cell antibody (AECA) in panel reactive antibody (PRA) negative recipients and its impact on AR within 6 months following renal transplantation. Methods: We retrospectively examined pre-transplant sera from 392 patients using cellular enzyme linked immunosorbent assay (ELISA) with substrate from a permanent endothelial cell line EAhy926. Equal volumes of serum from 40 healthy volunteers were mixed and used as the negative control. Results: The positive rate of AECA was 15.8%. There were no significant differences with respect to age, gender, original disease, dialysis history, immune suppressive regimen, cytomegalovirus (CMV) antigen positive rate, complement dependent cytotoxicity (CDC) level and soluble CD30 (sCD30) levels between the AECA positive group and AECA negative group. AR rate in the AECA positive group was higher than that in the AECA negative group (35.5% vs. 22.4%, p=0.023). The AECA positive patients had significantly higher rates of acute grade II T-cell mediated rejection (TMR) and acute antibody mediated rejection (AMR) compared with AECA negative patients. The concentrations of sCD30, and AECA were independent risk factors for AR within 6 months; the odds ratios were 7.005 and 2.469, respectively. Conclusions: Positive AECA was an independent risk factor for AR and appeared to correlate with relatively severe rejection subtypes. Clin Chem Lab Med 2009;47:1265–9.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titlePre-transplant serum concentrations of anti-endothelial cell antibody in panel reactive antibody negative renal recipients and its impact on acute rejection
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100119767
dc.identifier.doihttp://dx.doi.org/10.18452/9938
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-year2009
dc.description.versionPeer Reviewed
dcterms.bibliographicCitation.doi10.1515/CCLM.2009.283
dcterms.bibliographicCitation.journaltitleClinical Chemistry and Laboratory Medicine
dcterms.bibliographicCitation.volume47
dcterms.bibliographicCitation.issue10
dcterms.bibliographicCitation.originalpublishernamede Gruyter
dcterms.bibliographicCitation.pagestart1265
dcterms.bibliographicCitation.pageend1269

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