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2009-06-19Zeitschriftenartikel DOI: 10.1515/CCLM.2009.255
Isolation and expression of UB05, a Plasmodium falciparum antigen recognised by antibodies from semi-immune adults in a high transmission endemic area of the Cameroonian rainforest
dc.contributor.authorTitanji, Vincent Pryde Kehdinga
dc.contributor.authorAmambua-Ngwa, Alfred
dc.contributor.authorAnong, Damian Nota
dc.contributor.authorMbandi, Stanley Kimbeng
dc.contributor.authorTangie, Emily
dc.contributor.authorTening, Ivo
dc.contributor.authorYengo, Raymond
dc.date.accessioned2017-06-17T05:29:08Z
dc.date.available2017-06-17T05:29:08Z
dc.date.created2010-07-01
dc.date.issued2009-06-19
dc.identifier.issn1437-4331
dc.identifier.urihttp://edoc.hu-berlin.de/18452/11420
dc.description.abstractBackground: Antibodies in adults living in malaria endemic areas that target specific parasite antigens are implicated in protective immunity to infection and disease. This study aimed to identify, isolate and characterise targets of protective immunity in malaria. A Plasmodium falciparum antigen termed UB05 (Genbank Accession Number DQ235690: PlasmoDB PF10_ 0372) that had been isolated by immunoscreening with semi-immune sera was studied. Methods: Polymerase chain reaction, sequencing and bioinformatics were used to analyse the UB05 gene. A specific mouse anti-UB05 antibody was used in parasite reinvasion growth/inhibition assays and in immunoflourescence to localise the antigen. In a cross-sectional study, enzyme linked immunosorbent assay was used to study immunoglobulin G (IgG) responses to the antigen. Results: The gene revealed significant homologies with gene sequences from Plasmodia and other apicomplexan parasites and had two alleles in the wild P. falciparum isolates. The antigen is expressed by schizonts and segmented merozoites. Mouse antibodies against it marginally inhibit in vitro invasion of erythrocytes by P. falciparum. The IgG responses to UB05 were found to be significantly lower (p<0.05) in the sera of children (2–5 years) compared with adults (>18 years), with or without parasitaemia. However, parasitaemia correlated inversely (r=0.7– 0.75) with serum anti-UB05 IgG concentrations. Furthermore, anti-UB05 IgG concentrations were lower in the sera of febrile patients (body temperature >37.5°C) than their non-febrile counterparts regardless of parasitaemia status. Conclusions: These results are compatible with a role for UB05 in the development of immunity and as a marker of protective immunity to malaria. Clin Chem Lab Med 2009;47:1147–58.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleIsolation and expression of UB05, a Plasmodium falciparum antigen recognised by antibodies from semi-immune adults in a high transmission endemic area of the Cameroonian rainforest
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100132300
dc.identifier.doi10.1515/CCLM.2009.255
dc.identifier.doihttp://dx.doi.org/10.18452/10768
local.edoc.container-titleClinical Chemistry and Laboratory Medicine
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-namede Gruyter
local.edoc.container-volume47
local.edoc.container-issue9
local.edoc.container-year2009
local.edoc.container-firstpage1147
local.edoc.container-lastpage1158
dc.description.versionPeer Reviewed

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