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2009-05-04Zeitschriftenartikel DOI: 10.18452/11398
Gene expression profiling of maternal blood in early onset severe preeclampsia: identification of novel biomarkers
dc.contributor.authorSun, Cheng-Juan
dc.contributor.authorZhang, Liang
dc.contributor.authorZhang, Wei-Yuan
dc.date.accessioned2017-06-17T07:48:37Z
dc.date.available2017-06-17T07:48:37Z
dc.date.created2010-07-01
dc.date.issued2009-05-04
dc.identifier.issn1619-3997
dc.identifier.urihttp://edoc.hu-berlin.de/18452/12050
dc.description.abstractAims: To investigate candidate genes in peripheral blood mononuclear cell (PBMC) that are associated with early onset severe preeclampsia (ES-PE) and to describe candidate genes function using microarrays and real-time polymerase chain reaction (PCR). Methods: PBMC RNA was extracted from six patients with ES-PE and five uncomplicated pregnancies. The HG_U133 plus 2.0 Affymetrix GeneChips that represented 47,000 genes were used to measure gene expression in each sample. Significance analysis of microarray identified potential signature genes characterizing ES-PE vs. uncomplicated pregnancies. Eight genes were selected for confirmation by real-time PCR of 32 patients with ES-PE and 24 uncomplicated pregnancies, matched for maternal age, parity, race and gestational weeks. Results: Using a whole-genome approach to study the molecular determinants of ES-PE, 72 genes were found to be differentially expressed between cases and controls, including 38 up-regulated genes and 34 down-regulated genes in the group of ES-PE. Killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 2 (KIR3DL2), aldo-keto reductase family 1, member C3 (AKR1C3), churchill domain containing 1 (CHURC1), and solute carrier family 25, member 13 (SLC25A13) were validated to be down-regulated in the patients with ES-PE by real-time PCR. Conclusions: Expression of genes with diverse function is associated with ES-PE risk, providing opportunities for the development of non-invasive diagnosis.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleGene expression profiling of maternal blood in early onset severe preeclampsia: identification of novel biomarkers
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100142882
dc.identifier.doihttp://dx.doi.org/10.18452/11398
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-year2009
dc.description.versionPeer Reviewed
dcterms.bibliographicCitation.doi10.1515/JPM.2009.103
dcterms.bibliographicCitation.journaltitleJournal of Perinatal Medicine
dcterms.bibliographicCitation.volume37
dcterms.bibliographicCitation.issue6
dcterms.bibliographicCitation.originalpublishernamede Gruyter
dcterms.bibliographicCitation.pagestart609
dcterms.bibliographicCitation.pageend616

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