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2009-09-20Zeitschriftenartikel DOI: 10.1515/CCLM.2010.017
A study on the stability of urinary free catecholamines and free methyl-derivatives at different pH, temperature and time of storage
dc.contributor.authorRoberts, Norman B.
dc.contributor.authorHiggins, Gerald
dc.contributor.authorSargazi, Mansour
dc.date.accessioned2017-06-17T08:18:14Z
dc.date.available2017-06-17T08:18:14Z
dc.date.created2010-07-01
dc.date.issued2009-09-20
dc.identifier.issn1437-4331
dc.identifier.urihttp://edoc.hu-berlin.de/18452/12188
dc.description.abstractBakground: The goal of our study was to test the relative stability of urine, unconjugated, free catecholamines and the methyl derivatives. We measured the change in concentrations in commercially available urines after storage at various pH values, temperatures and time, from days up to 10 weeks. Methods: Samples of commercial control urines were adjusted to pH 2.0, 4.0, 6.0 and 8.0 and aliquots stored at ambient temperature (20–26°C), 4°C and –18oC. The free catecholamines (cats) and the free methyl derivatives (mets) were measured after 1, 2, 3 and 6 days and 1, 2, 3 and 10 weeks using the automated sample trace enrichment dialysis (ASTED) procedure with reversed phase ion pair high performance liquid chromatography (HPLC) and coulometric detection. Results: Free catecholamines were relatively stable, with <15% loss of concentration, when stored at pH 6.0 or less for at least 4 days and up to 10 weeks at pH 2.0 at either 4oC or –18oC. At pH 8.0, the concentration fell to <60% after 48 h and at a pH of 6.0 or 8.0, up to 90% was lost within the first week at 4oC and 25oC. More than 40% of free normetadrenaline and metadrenaline were lost after 1–2 weeks when stored at 20–25oC and pH 8.0. After 10 weeks at pH 4.0, 6.0 and 8.0, up to 90% loss was observed at 25oC. Free cats were stable at pH 2.0 and 4.0 at –18oC and the free mets were stable at –18oC over the entire time period studied and at all pHs. Conclusions: In the analysis of free catecholamine and the free methyl derivatives, urine samples should be acidified to a pH range 2.0–3.0 to ensure stability and hence the correct analysis. Clin Chem Lab Med 2010;48:81–7.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleA study on the stability of urinary free catecholamines and free methyl-derivatives at different pH, temperature and time of storage
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100145011
dc.identifier.doi10.1515/CCLM.2010.017
dc.identifier.doihttp://dx.doi.org/10.18452/11536
local.edoc.container-titleClinical Chemistry and Laboratory Medicine
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-namede Gruyter
local.edoc.container-volume48
local.edoc.container-issue1
local.edoc.container-year2010
local.edoc.container-firstpage81
local.edoc.container-lastpage87
dc.description.versionPeer Reviewed

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