Show simple item record

2009-05-12Zeitschriftenartikel DOI: 10.1515/CCLM.2009.167
Approaching clinical proteomics: current state and future fields of application in fluid proteomics
dc.contributor.authorApweiler, Rolf
dc.contributor.authorAslanidis, Charalampos
dc.contributor.authorDeufel, Thomas
dc.contributor.authorGerstner, Andreas
dc.contributor.authorHansen, Jens
dc.contributor.authorHochstrasser, Dennis
dc.contributor.authorKellner, Roland
dc.contributor.authorKubicek, Markus
dc.contributor.authorLottspeich, Friedrich
dc.contributor.authorMaser, Edmund
dc.contributor.authorMewes, Hans-Werner
dc.contributor.authorMeyer, Helmut E.
dc.contributor.authorMüllner, Stefan
dc.contributor.authorMutter, Wolfgang
dc.contributor.authorNeumaier, Michael
dc.contributor.authorNollau, Peter
dc.contributor.authorNothwang, Hans G.
dc.contributor.authorPonten, Fredrik
dc.contributor.authorRadbruch, Andreas
dc.contributor.authorReinert, Knut
dc.contributor.authorRothe, Gregor
dc.contributor.authorStockinger, Hannes
dc.contributor.authorTarnok, Attila
dc.contributor.authorTaussig, Mike J.
dc.contributor.authorThiel, Andreas
dc.contributor.authorThiery, Joachim
dc.contributor.authorUeffing, Marius
dc.contributor.authorValet, Günther
dc.contributor.authorVandekerckhove, Joel
dc.contributor.authorVerhuven, Wiltrud
dc.contributor.authorWagener, Christoph
dc.contributor.authorWagner, Oswald
dc.contributor.authorSchmitz, Gerd
dc.date.accessioned2017-06-17T10:30:50Z
dc.date.available2017-06-17T10:30:50Z
dc.date.created2010-07-01
dc.date.issued2009-05-12
dc.identifier.issn1437-4331
dc.identifier.urihttp://edoc.hu-berlin.de/18452/12770
dc.description.abstractThe field of clinical proteomics offers opportunities to identify new disease biomarkers in body fluids, cells and tissues. These biomarkers can be used in clinical applications for diagnosis, stratification of patients for specific treatment, or therapy monitoring. New protein array formats and improved spectrometry technologies have brought these analyses to a level with potential for use in clinical diagnostics. The nature of the human body fluid proteome with its large dynamic range of protein concentrations presents problems with quantitation. The extreme complexity of the proteome in body fluids presents enormous challenges and requires the establishment of standard operating procedures for handling of specimens, increasing sensitivity for detection and bioinformatical tools for distribution of proteomic data into the public domain. From studies of in vitro diagnostics, especially in clinical chemistry, it is evident that most errors occur in the preanalytical phase and during implementation of the diagnostic strategy. This is also true for clinical proteomics, and especially for fluid proteomics because of the multiple pretreatment processes. These processes include depletion of high-abundance proteins from plasma or enrichment processes for urine where biological variation or differences in proteolytic activities in the sample along with preanalytical variables such as inter- and intra-assay variability will likely influence the results of proteomics studies. However, before proteomic analysis can be introduced at a broader level into the clinical setting, standardization of the preanalytical phase including patient preparation, sample collection, sample preparation, sample storage, measurement and data analysis needs to be improved. In this review, we discuss the recent technological advances and applications that fulfil the criteria for clinical proteomics, with the focus on fluid proteomics. These advances relate to preanalytical factors, analytical standardization and quality-control measures required for effective implementation into routine laboratory testing in order to generate clinically useful information. With new disease biomarker candidates, it will be crucial to design and perform clinical studies that can identify novel diagnostic strategies based on these techniques, and to validate their impact on clinical decision-making. Clin Chem Lab Med 2009;47:724–44.eng
dc.language.isound
dc.publisherKooperation de Gruyter
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.titleApproaching clinical proteomics: current state and future fields of application in fluid proteomics
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-100153690
dc.identifier.doi10.1515/CCLM.2009.167
dc.identifier.doihttp://dx.doi.org/10.18452/12118
local.edoc.container-titleClinical Chemistry and Laboratory Medicine
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-namede Gruyter
local.edoc.container-volume47
local.edoc.container-issue6
local.edoc.container-year2009
local.edoc.container-firstpage724
local.edoc.container-lastpage744
dc.description.versionPeer Reviewed

Show simple item record