Stabilty Constants of Haemiglobin Cyanide and Azide Measured by Two-Wavelength Spectrophotometric Method

Abstract

A two-wavelength spectrophotometric procedure for the simultaneous determination of haemiglobin and haemiglobin cyanide (HiCN( (or of haemiglobin and haemiglobin azide (HiN3)) concentrations in mixtures has been developed and applied to the determination of the stability constants of HiCN and HiN3. The analytically reliable procedure allowed stablity constants to be estimated with about 10% (relative standard deviation, coefficient of variation) uncertainty.