A Sensitive Method for Determination of Conjugates Catecholamines in Blood Plasma

Abstract

Conjugated catecholamines in blood plasma of blood donors were determined from the difference between the total and the free catecholamines. Total catecholamines were measured after complete hydrolysis of the conjugated amines (heating of acidified samples for 40 min at 95°C). Free catecholamines were determined in untreated plasma. Determinations were performed radioenzymatically. We combined and modified the procedures of Peuler & Johnson (1977) Life Sei 27, 625-636) and Da Prada & Z rcher (1976) Life Sei 29, 1161—1174). The resulting method was less laborious and cheaper.