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2015-10-19Zeitschriftenartikel DOI: 10.18452/18571
Dual-fluorescence pH probe for bio-labelling
dc.contributor.authorRichter, C.
dc.contributor.authorSchneider, C.
dc.contributor.authorQuick, M. T.
dc.contributor.authorVolz, P.
dc.contributor.authorMahrwald, Rainer
dc.contributor.authorHughes, J.
dc.contributor.authorDick, B.
dc.contributor.authorAlexiev, U.
dc.contributor.authorErnsting, Nikolaus
dc.date.accessioned2017-11-24T10:25:33Z
dc.date.available2017-11-24T10:25:33Z
dc.date.issued2015-10-19
dc.identifier.other10.1039/C5CP05454K
dc.identifier.urihttp://edoc.hu-berlin.de/18452/19267
dc.description.abstractAlthough seminaphtorhodafluor (SNARF) dyes are already widely used to measure pH in cells and at biofilms, their synthesis has low yield and results in an unspecific position of a carboxy-group. The separation of 5′- and 6′-carboxy-SNARF reveals a pKa difference of 0.15, calling into question pH measurements with the (commercially available) mixture. Here we replace the bulky external dicarboxyphenyl ring with a propionate group and evaluate the spectral properties of the new derivative. Proceeding to the ethyl-iodoacetamide, covalent linkage to cysteine protein sites is achieved efficiently as shown with a cyanobacterial phytochrome, extending the scarce application of SNARF in bio-labelling in the current literature. Application in fluorescence lifetime imaging is demonstrated both with the lifetime-based and ratiometric-yield method.eng
dc.language.isoeng
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 3.0) Namensnennung 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject.ddc541 Physikalische Chemie
dc.titleDual-fluorescence pH probe for bio-labelling
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/19267-7
dc.identifier.doihttp://dx.doi.org/10.18452/18571
dc.type.versionpublishedVersion
local.edoc.container-titlePhysical Chemistry Chemical Physics
local.edoc.pages8
local.edoc.anmerkungAvailable open access thanks to the RSC Gold for Gold initiative. Shared according to the terms set out in the CC licence.
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionMathematisch-Naturwissenschaftliche Fakultät
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://pubs.rsc.org/en/journals/journalissues/cp
local.edoc.container-publisher-nameThe Royal Society of Chemistry
local.edoc.container-volume17
local.edoc.container-issue45
local.edoc.container-firstpage30590
local.edoc.container-lastpage30597
dc.description.versionPeer Reviewed

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