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2015-10-19Zeitschriftenartikel DOI: 10.18452/18571
Dual-fluorescence pH probe for bio-labelling
dc.contributor.authorRichter, C.
dc.contributor.authorSchneider, C.
dc.contributor.authorQuick, M. T.
dc.contributor.authorVolz, P.
dc.contributor.authorMahrwald, Rainer
dc.contributor.authorHughes, J.
dc.contributor.authorDick, B.
dc.contributor.authorAlexiev, U.
dc.contributor.authorErnsting, Nikolaus
dc.date.accessioned2017-11-24T10:25:33Z
dc.date.available2017-11-24T10:25:33Z
dc.date.issued2015-10-19
dc.identifier.urihttp://edoc.hu-berlin.de/18452/19267
dc.descriptionAvailable open access thanks to the RSC Gold for Gold initiative. Shared according to the terms set out in the CC licence.
dc.description.abstractAlthough seminaphtorhodafluor (SNARF) dyes are already widely used to measure pH in cells and at biofilms, their synthesis has low yield and results in an unspecific position of a carboxy-group. The separation of 5′- and 6′-carboxy-SNARF reveals a pKa difference of 0.15, calling into question pH measurements with the (commercially available) mixture. Here we replace the bulky external dicarboxyphenyl ring with a propionate group and evaluate the spectral properties of the new derivative. Proceeding to the ethyl-iodoacetamide, covalent linkage to cysteine protein sites is achieved efficiently as shown with a cyanobacterial phytochrome, extending the scarce application of SNARF in bio-labelling in the current literature. Application in fluorescence lifetime imaging is demonstrated both with the lifetime-based and ratiometric-yield method.eng
dc.language.isoeng
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 3.0) Namensnennung 3.0 Unported
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject.ddc541 Physikalische Chemie
dc.titleDual-fluorescence pH probe for bio-labelling
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/19267-7
dc.identifier.doihttp://dx.doi.org/10.18452/18571
dc.type.versionpublishedVersion
local.edoc.pages8
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewed
dcterms.bibliographicCitation.doi10.1039/C5CP05454K
dcterms.bibliographicCitation.journaltitlePhysical Chemistry Chemical Physics
dcterms.bibliographicCitation.volume17
dcterms.bibliographicCitation.issue45
dcterms.bibliographicCitation.originalpublishernameThe Royal Society of Chemistry
dcterms.bibliographicCitation.pagestart30590
dcterms.bibliographicCitation.pageend30597
bua.departmentMathematisch-Naturwissenschaftliche Fakultät

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