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2018-05-01Zeitschriftenartikel DOI: 10.18452/19610
Regulation of Flagellum Biosynthesis in Response to Cell Envelope Stress in Salmonella enterica Serovar Typhimurium
dc.contributor.authorSpöring, Imke
dc.contributor.authorFelgner, Sebastian
dc.contributor.authorPreuße, Matthias
dc.contributor.authorEckweiler, Denitsa
dc.contributor.authorRohde, Manfred
dc.contributor.authorHäussler, Susanne
dc.contributor.authorWeiss, Siegfried
dc.contributor.authorErhardt, Marc
dc.date.accessioned2018-12-12T15:20:28Z
dc.date.available2018-12-12T15:20:28Z
dc.date.issued2018-05-01
dc.identifier.issn2150-7511
dc.identifier.other10.1128/mBio.00736-17
dc.identifier.urihttp://edoc.hu-berlin.de/18452/20380
dc.description.abstractFlagellum-driven motility of Salmonella enterica serovar Typhimurium facilitates host colonization. However, the large extracellular flagellum is also a prime target for the immune system. As consequence, expression of flagella is bistable within a population of Salmonella, resulting in flagellated and nonflagellated subpopulations. This allows the bacteria to maximize fitness in hostile environments. The degenerate EAL domain protein RflP (formerly YdiV) is responsible for the bistable expression of flagella by directing the flagellar master regulatory complex FlhD4C2 with respect to proteolytic degradation. Information concerning the environmental cues controlling expression of rflP and thus about the bistable flagellar biosynthesis remains ambiguous. Here, we demonstrated that RflP responds to cell envelope stress and alterations of outer membrane integrity. Lipopolysaccharide (LPS) truncation mutants of Salmonella Typhimurium exhibited increasing motility defects due to downregulation of flagellar gene expression. Transposon mutagenesis and genetic profiling revealed that σ24 (RpoE) and Rcs phosphorelay-dependent cell envelope stress response systems sense modifications of the lipopolysaccaride, low pH, and activity of the complement system. This subsequently results in activation of RflP expression and degradation of FlhD4C2 via ClpXP. We speculate that the presence of diverse hostile environments inside the host might result in cell envelope damage and would thus trigger the repression of resource-costly and immunogenic flagellum biosynthesis via activation of the cell envelope stress response.eng
dc.language.isoeng
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.ddc570 Biologie
dc.titleRegulation of Flagellum Biosynthesis in Response to Cell Envelope Stress in Salmonella enterica Serovar Typhimurium
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/20380-3
dc.identifier.doihttp://dx.doi.org/10.18452/19610
dc.type.versionpublishedVersion
local.edoc.container-titlemBio
local.edoc.pages17
local.edoc.anmerkungThis article was supported by the German Research Foundation (DFG) and the Open Access Publication Fund of Humboldt-Universität zu Berlin.
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultät
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-publisher-placeWashington, DC
local.edoc.container-volumeVolume 9
local.edoc.container-issueIssue 3
dc.description.versionPeer Reviewed
local.edoc.container-articlenumbere00736-17

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