The international workshop entitled “Biodiversity and the urban-rural interface: conflicts vs. opportunities” took place between the 24th and the 27th of September 2018 in Linde, Germany. This workshop marks the third annual academic event within our project “Land use contrasts and edge effects – a comparative approach”, affiliated with the Systematic Zoology Division at Humboldt-Universität zu Berlin and supported by the Zwillenberg-Tietz Stiftung (foundation). The event follows on from the discussions and results achieved within the previous workshops of this project, entitled “Biodiversity and land use – a comparative approach” and “Connecting biodiversity and improving human livelihood beyond boundaries – a global perspective”. Our third workshop aims at comparatively examining interrelations between land use and biodiversity using the example of urban-rural interfaces.