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2017-02-10Zeitschriftenartikel DOI: 10.18452/20345
Cross-Talk between Dnmt2-Dependent tRNA Methylation and Queuosine Modification
dc.contributor.authorEhrenhofer-Murray, Ann Elizabeth
dc.date.accessioned2019-08-01T11:37:18Z
dc.date.available2019-08-01T11:37:18Z
dc.date.issued2017-02-10none
dc.date.updated2019-07-29T16:22:58Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21103
dc.description.abstractEnzymes of the Dnmt2 family of methyltransferases have yielded a number of unexpected discoveries. The first surprise came more than ten years ago when it was realized that, rather than being DNA methyltransferases, Dnmt2 enzymes actually are transfer RNA (tRNA) methyltransferases for cytosine-5 methylation, foremost C38 (m5C38) of tRNAAsp. The second unanticipated finding was our recent discovery of a nutritional regulation of Dnmt2 in the fission yeast Schizosaccharomyces pombe. Significantly, the presence of the nucleotide queuosine in tRNAAsp strongly stimulates Dnmt2 activity both in vivo and in vitro in S. pombe. Queuine, the respective base, is a hypermodified guanine analog that is synthesized from guanosine-5’-triphosphate (GTP) by bacteria. Interestingly, most eukaryotes have queuosine in their tRNA. However, they cannot synthesize it themselves, but rather salvage it from food or from gut microbes. The queuine obtained from these sources comes from the breakdown of tRNAs, where the queuine ultimately was synthesized by bacteria. Queuine thus has been termed a micronutrient. This review summarizes the current knowledge of Dnmt2 methylation and queuosine modification with respect to translation as well as the organismal consequences of the absence of these modifications. Models for the functional cooperation between these modifications and its wider implications are discussed.eng
dc.description.sponsorshipDeutsche Forschungsgemeinschaft
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDnmt2eng
dc.subjectPmt1eng
dc.subjectqueuineeng
dc.subjectqueuosineeng
dc.subjecttranslationeng
dc.subjecttRNA cleavageeng
dc.subjectepitranscriptomicseng
dc.subjectanticodon modificationeng
dc.subject.ddc570 Biologienone
dc.titleCross-Talk between Dnmt2-Dependent tRNA Methylation and Queuosine Modificationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21103-8
dc.identifier.doihttp://dx.doi.org/10.18452/20345
dc.type.versionpublishedVersionnone
local.edoc.pages21none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn2218-273X
dcterms.bibliographicCitation.doi10.3390/biom7010014none
dcterms.bibliographicCitation.journaltitleBiomoleculesnone
dcterms.bibliographicCitation.volume7none
dcterms.bibliographicCitation.issue1none
dcterms.bibliographicCitation.originalpublishernameMDPInone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
dcterms.bibliographicCitation.pagestart14/1none
dcterms.bibliographicCitation.pageend14/21none
bua.import.affiliationEhrenhofer-Murray, Ann; Institut für Biologie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany,none
bua.departmentLebenswissenschaftliche Fakultätnone

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