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2018-01-25Zeitschriftenartikel DOI: 10.18452/20378
Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
dc.contributor.authorZeise, Ingrid
dc.contributor.authorHeiner, Zsuzsanna
dc.contributor.authorHolz, Sabine
dc.contributor.authorJoester, Maike
dc.contributor.authorBüttner, Carmen
dc.contributor.authorKneipp, Janina
dc.date.accessioned2019-08-08T08:53:01Z
dc.date.available2019-08-08T08:53:01Z
dc.date.issued2018-01-25none
dc.date.updated2019-07-31T17:05:22Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21129
dc.description.abstractRaman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCucumis sativuseng
dc.subjectcell walleng
dc.subjectxylemeng
dc.subjectRaman spectroscopyeng
dc.subjectchemical imagingeng
dc.subjectcelluloseeng
dc.subjectlignineng
dc.subjectprincipal component analysiseng
dc.subjecthierarchical cluster analysiseng
dc.subjectcaroteneeng
dc.subject.ddc580 Pflanzen (Botanik)none
dc.titleRaman Imaging of Plant Cell Walls in Sections of Cucumis sativusnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21129-7
dc.identifier.doihttp://dx.doi.org/10.18452/20378
dc.type.versionpublishedVersionnone
local.edoc.pages16none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn2223-7747
dcterms.bibliographicCitation.doi10.3390/plants7010007none
dcterms.bibliographicCitation.journaltitlePlantsnone
dcterms.bibliographicCitation.volume7none
dcterms.bibliographicCitation.issue1none
dcterms.bibliographicCitation.originalpublishernameMDPInone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
dcterms.bibliographicCitation.pagestart7/1none
dcterms.bibliographicCitation.pageend7/16none
bua.import.affiliationZeise, Ingrid; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany,none
bua.import.affiliationHeiner, Zsuzsanna; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany, School of Analytical Sciences Adlershof SALSA, Humboldt-Universität zu Berlin, Albert-Einstein-Str. 5-9, 12489 Berlin, Germany,none
bua.import.affiliationHolz, Sabine; Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Lentzeallee 55/57, 14195 Berlin, Germany,none
bua.import.affiliationJoester, Maike; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany, BAM Federal Institute for Materials Research and Testing, Richard-Willstatter-Straße 11, 12489 Berlin, Germany,none
bua.import.affiliationBüttner, Carmen; Institute of Agricultural and Horticultural Sciences, Humboldt-Universität zu Berlin, Lentzeallee 55/57, 14195 Berlin, Germany,none
bua.import.affiliationKneipp, Janina; Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489 Berlin, Germany, School of Analytical Sciences Adlershof SALSA, Humboldt-Universität zu Berlin, Albert-Einstein-Str. 5-9, 12489 Berlin, Germany,none
bua.departmentMathematisch-Naturwissenschaftliche Fakultätnone

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