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2018-07-02Zeitschriftenartikel DOI: 10.18452/20400
Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content
dc.contributor.authorKurze, Elisabeth
dc.contributor.authorKock, Vanessa
dc.contributor.authorLo Scalzo, Roberto
dc.contributor.authorOlbricht, Klaus
dc.contributor.authorSchwab, Wilfried
dc.date.accessioned2019-08-14T11:58:29Z
dc.date.available2019-08-14T11:58:29Z
dc.date.issued2018-07-02none
dc.date.updated2019-08-01T00:32:20Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21149
dc.description.abstractBirch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and seasonal effects were minor.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectstrawberryeng
dc.subjectfood allergyeng
dc.subjectFra a 1eng
dc.subjectpathogenesis-related proteinseng
dc.subjectindirect competitive ELISAeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEffect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Contentnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21149-9
dc.identifier.doihttp://dx.doi.org/10.18452/20400
dc.type.versionpublishedVersionnone
local.edoc.pages15none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn2072-6643
dcterms.bibliographicCitation.doi10.3390/nu10070857none
dcterms.bibliographicCitation.journaltitleNutrientsnone
dcterms.bibliographicCitation.volume10none
dcterms.bibliographicCitation.issue7none
dcterms.bibliographicCitation.originalpublishernameMDPInone
dcterms.bibliographicCitation.originalpublisherplaceBaselnone
dcterms.bibliographicCitation.pagestart857/1none
dcterms.bibliographicCitation.pageend857/15none
bua.import.affiliationKurze, Elisabeth; Biotechnology of Natural Products, Technische Universität München, Liesel-Beckmann-Str.1, 85354 Freising, Germany,none
bua.import.affiliationKock, Vanessa; Biotechnology of Natural Products, Technische Universität München, Liesel-Beckmann-Str.1, 85354 Freising, Germany,none
bua.import.affiliationLo Scalzo, Roberto; Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Unità di ricerca per i processi dell’industria agroalimentare (CREA-IAA), via Venezian 26, 20133 Milan, Italy,none
bua.import.affiliationOlbricht, Klaus; Hansabred GmbH & Co. KG, Radeburger Landstr. 12, 01108 Dresden, Germany, Humboldt-Universität zu Berlin, Albrecht Daniel Thaer-Institute, 10099 Berlin, Germany,none
bua.import.affiliationSchwab, Wilfried; Biotechnology of Natural Products, Technische Universität München, Liesel-Beckmann-Str.1, 85354 Freising, Germany,none
bua.departmentLebenswissenschaftliche Fakultätnone

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