Show simple item record

2018-07-09Zeitschriftenartikel DOI: 10.3390/toxins10070284
Toxicity Assay for Citrinin, Zearalenone and Zearalenone-14-Sulfate Using the Nematode Caenorhabditis elegans as Model Organism
dc.contributor.authorKeller, Julia
dc.contributor.authorBorzekowski, Antje
dc.contributor.authorHaase, Hajo
dc.contributor.authorMenzel, Ralph
dc.contributor.authorRuess, Liliane
dc.contributor.authorKoch, Matthias
dc.date.accessioned2019-08-19T09:11:09Z
dc.date.available2019-08-19T09:11:09Z
dc.date.issued2018-07-09none
dc.date.updated2019-08-01T01:23:46Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21154
dc.description.abstractTo keep pace with the rising number of detected mycotoxins, there is a growing need for fast and reliable toxicity tests to assess potential threats to food safety. Toxicity tests with the bacterial-feeding nematode Caenorhabditis elegans as the model organism are well established. In this study the C. elegans wildtype strain N2 (var. Bristol) was used to investigate the toxic effects of the food-relevant mycotoxins citrinin (CIT) and zearalenone-14-sulfate (ZEA-14-S) and zearalenone (ZEA) on different life cycle parameters including reproduction, thermal and oxidative stress resistance and lifespan. The metabolization of the mycotoxins by the nematodes in vivo was investigated using HPLC-MS/MS. ZEA was metabolized in vivo to the reduced isomers α-zearalenol (α-ZEL) and β-ZEL. ZEA-14-S was reduced to α-/β-ZEL-14-sulfate and CIT was metabolized to mono-hydroxylated CIT. All mycotoxins tested led to a significant decrease in the number of nematode offspring produced. ZEA and CIT displayed negative effects on stress tolerance levels and for CIT an additional shortening of the mean lifespan was observed. In the case of ZEA-14-S, however, the mean lifespan was prolonged. The presented study shows the applicability of C. elegans for toxicity testing of emerging food mycotoxins for the purpose of assigning potential health threats.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectmycotoxineng
dc.subjecttoxicity testingeng
dc.subjectfood safetyeng
dc.subjectCaenorhabditis eleganseng
dc.subjectmetabolizationeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleToxicity Assay for Citrinin, Zearalenone and Zearalenone-14-Sulfate Using the Nematode Caenorhabditis elegans as Model Organismnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21154-3
dc.identifier.doi10.3390/toxins10070284none
dc.identifier.doihttp://dx.doi.org/10.18452/20404
dc.type.versionpublishedVersionnone
local.edoc.container-titleToxinsnone
local.edoc.pages12none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameMDPInone
local.edoc.container-publisher-placeBaselnone
local.edoc.container-volume10none
local.edoc.container-issue7none
local.edoc.container-firstpage284/1none
local.edoc.container-lastpage284/12none
dc.description.versionPeer Reviewednone
dc.identifier.eissn2072-6651
local.edoc.affiliationKeller, Julia; Department of Analytical Chemistry, Reference Materials, Bundesanstalt für Materialforschung und-Prüfung (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany,none
local.edoc.affiliationBorzekowski, Antje; Department of Analytical Chemistry, Reference Materials, Bundesanstalt für Materialforschung und-Prüfung (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany,none
local.edoc.affiliationHaase, Hajo; Department of Food Chemistry and Toxicology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355 Berlin, Germany,none
local.edoc.affiliationMenzel, Ralph; Institute of Biology, Ecology, Humboldt-Universität zu Berlin, Philippstr. 13, 10115 Berlin, Germany,none
local.edoc.affiliationRueß, Liliane; Institute of Biology, Ecology, Humboldt-Universität zu Berlin, Philippstr. 13, 10115 Berlin, Germany,none
local.edoc.affiliationKoch, Matthias; Department of Analytical Chemistry, Reference Materials, Bundesanstalt für Materialforschung und-Prüfung (BAM), Richard-Willstätter-Str. 11, 12489 Berlin, Germany,none

Show simple item record