Logo of Humboldt-Universität zu BerlinLogo of Humboldt-Universität zu Berlin
edoc-Server
Open-Access-Publikationsserver der Humboldt-Universität
de|en
Header image: facade of Humboldt-Universität zu Berlin
View Item 
  • edoc-Server Home
  • Artikel und Monographien
  • Zweitveröffentlichungen
  • View Item
  • edoc-Server Home
  • Artikel und Monographien
  • Zweitveröffentlichungen
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.
All of edoc-ServerCommunity & CollectionTitleAuthorSubjectThis CollectionTitleAuthorSubject
PublishLoginRegisterHelp
StatisticsView Usage Statistics
All of edoc-ServerCommunity & CollectionTitleAuthorSubjectThis CollectionTitleAuthorSubject
PublishLoginRegisterHelp
StatisticsView Usage Statistics
View Item 
  • edoc-Server Home
  • Artikel und Monographien
  • Zweitveröffentlichungen
  • View Item
  • edoc-Server Home
  • Artikel und Monographien
  • Zweitveröffentlichungen
  • View Item
2012-08-27Zeitschriftenartikel DOI: 10.3389/fimmu.2012.00264
IL-2 Expression in Activated Human Memory FOXP3+ Cells Critically Depends on the Cellular Levels of FOXP3 as Well as of Four Transcription Factors of  T Cell Activation
Bendfeldt, Hanna
Benary, Manuela
Scheel, Tobias
Steinbrink, Kerstin
Radbruch, Andreas
Herzel, Hanspeter
Baumgrass, Ria cc
Lebenswissenschaftliche Fakultät
The human CD4+FOXP3+ T cell population is heterogeneous and consists of various subpopulations which remain poorly defined. Anergy and suppression are two main functional characteristics of FOXP3+Treg cells. We used the anergic behavior of FOXP3+Treg cells for a better discrimination and characterization of such subpopulations. We compared IL-2-expressing with IL-2-non-expressing cells within the memory FOXP3+ T cell population. In contrast to IL-2-non-expressing FOXP3+ cells, IL-2-expressing FOXP3+ cells exhibit intermediate characteristics of Treg and Th cells concerning the Treg cell markers CD25, GITR, and Helios. Besides lower levels of FOXP3, they also have higher levels of the transcription factors NFATc2, c-Fos, NF-κBp65, and c-Jun. An approach combining flow cytometric measurements with statistical interpretation for quantitative transcription factor analysis suggests that the physiological expression levels not only of FOXP3 but also of NFATc2, c-Jun, c-Fos, and NF-κBp65 are limiting for the decision whether IL-2 is expressed or not in activated peripheral human memory FOXP3+ cells. These findings demonstrate that concomitant high levels of NFATc2, c-Jun, c-Fos, and NF-κBp65 lead in addition to potential IL-2 expression in those FOXP3+ cells with low levels of FOXP3. We hypothesize that not only the level of FOXP3 expression but also the amounts of the four transcription factors studied represent determining factors for the anergic phenotype of FOXP3+ Treg cells.
Files in this item
Thumbnail
fimmu-03-00264.pdf — Adobe PDF — 2.198 Mb
MD5: 4646bb6b24a3a725cf5749fc032a2724
fimmu-03-00264-g001.tif — TIFF image — 26.41 Mb
MD5: 931e88be35eaa781d5fdbe5a98b911f1
fimmu-03-00264-g002.tif — TIFF image — 17.16 Mb
MD5: 4828be8ee01ef77473b296172a2d1bb6
fimmu-03-00264-g003.tif — TIFF image — 27.58 Mb
MD5: 7cf816314a5e0c9daa6779efec5f2622
fimmu-03-00264-g004.tif — TIFF image — 24.84 Mb
MD5: 82504c558124e6c1f67e4c2c6893f974
fimmu-03-00264-g005.tif — TIFF image — 7.013 Mb
MD5: 867ec428586e0c616707a983102713f8
fimmu-03-00264-g006.tif — TIFF image — 11.39 Mb
MD5: 9148e5cd56d743fd0769e87af8220e72
fimmu-03-00264-g007.tif — TIFF image — 17.21 Mb
MD5: 8c86546fc510188558f37b58ef3144e7
Cite
BibTeX
EndNote
RIS
(CC BY 3.0) Attribution 3.0 Unported(CC BY 3.0) Attribution 3.0 Unported
Details
DINI-Zertifikat 2019OpenAIRE validatedORCID Consortium
Imprint Policy Contact Data Privacy Statement
A service of University Library and Computer and Media Service
© Humboldt-Universität zu Berlin
 
DOI
10.3389/fimmu.2012.00264
Permanent URL
https://doi.org/10.3389/fimmu.2012.00264
HTML
<a href="https://doi.org/10.3389/fimmu.2012.00264">https://doi.org/10.3389/fimmu.2012.00264</a>