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2012-08-27Zeitschriftenartikel DOI: 10.18452/20715
IL-2 Expression in Activated Human Memory FOXP3+ Cells Critically Depends on the Cellular Levels of FOXP3 as Well as of Four Transcription Factors of  T Cell Activation
dc.contributor.authorBendfeldt, Hanna
dc.contributor.authorBenary, Manuela
dc.contributor.authorScheel, Tobias
dc.contributor.authorSteinbrink, Kerstin
dc.contributor.authorRadbruch, Andreas
dc.contributor.authorHerzel, Hanspeter
dc.contributor.authorBaumgrass, Ria
dc.date.accessioned2019-11-06T10:52:16Z
dc.date.available2019-11-06T10:52:16Z
dc.date.issued2012-08-27none
dc.date.updated2019-08-12T13:04:16Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21440
dc.description.abstractThe human CD4+FOXP3+ T cell population is heterogeneous and consists of various subpopulations which remain poorly defined. Anergy and suppression are two main functional characteristics of FOXP3+Treg cells. We used the anergic behavior of FOXP3+Treg cells for a better discrimination and characterization of such subpopulations. We compared IL-2-expressing with IL-2-non-expressing cells within the memory FOXP3+ T cell population. In contrast to IL-2-non-expressing FOXP3+ cells, IL-2-expressing FOXP3+ cells exhibit intermediate characteristics of Treg and Th cells concerning the Treg cell markers CD25, GITR, and Helios. Besides lower levels of FOXP3, they also have higher levels of the transcription factors NFATc2, c-Fos, NF-κBp65, and c-Jun. An approach combining flow cytometric measurements with statistical interpretation for quantitative transcription factor analysis suggests that the physiological expression levels not only of FOXP3 but also of NFATc2, c-Jun, c-Fos, and NF-κBp65 are limiting for the decision whether IL-2 is expressed or not in activated peripheral human memory FOXP3+ cells. These findings demonstrate that concomitant high levels of NFATc2, c-Jun, c-Fos, and NF-κBp65 lead in addition to potential IL-2 expression in those FOXP3+ cells with low levels of FOXP3. We hypothesize that not only the level of FOXP3 expression but also the amounts of the four transcription factors studied represent determining factors for the anergic phenotype of FOXP3+ Treg cells.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 3.0) Attribution 3.0 Unportedger
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectcytokine expressioneng
dc.subjecttranscription factorseng
dc.subjectT cell activationeng
dc.subjectIL-2 expressioneng
dc.subjectlymphocyteeng
dc.subjectflow cytometryeng
dc.subjecthuman Treg cellseng
dc.subjectmemory Th cellseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleIL-2 Expression in Activated Human Memory FOXP3+ Cells Critically Depends on the Cellular Levels of FOXP3 as Well as of Four Transcription Factors of  T Cell Activationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21440-1
dc.identifier.doihttp://dx.doi.org/10.18452/20715
dc.type.versionpublishedVersionnone
local.edoc.pages10none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1664-3224
dcterms.bibliographicCitation.doi10.3389/fimmu.2012.00264none
dcterms.bibliographicCitation.journaltitleFrontiers in immunologynone
dcterms.bibliographicCitation.volume3none
dcterms.bibliographicCitation.articlenumber264none
dcterms.bibliographicCitation.originalpublishernameFrontiers Research Foundationnone
dcterms.bibliographicCitation.originalpublisherplaceLausannenone
bua.departmentLebenswissenschaftliche Fakultätnone

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