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2019-01Zeitschriftenartikel DOI: 10.18452/19934
Molecular characterization of the movement and coat proteins of a new Elm mottle virus isolate infecting European white elm (Ulmus laevis Pall.)
dc.contributor.authorEisold, Anne-Mareen E.
dc.date.accessioned2019-11-11T09:19:03Z
dc.date.available2019-11-11T09:19:03Z
dc.date.issued2019-01none
dc.identifier.issnISSN: 2312-9344
dc.identifier.other10.33687/phytopath.008.01.2742
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21466
dc.description.abstractEuropean white elms (Ulmus laevis Pall.) growing in a park in Caputh near Berlin (Germany) were regularly monitored over a period of 18 years showing virus infection-like symptoms such as chloroses, chlorotic ringspots, mottling and dieback. To obtain the evidence for viral infection, RNA-seq using an Illumina Hi Seq2500 was conducted and three contigs were obtained. They match with the three EMoV genomic RNAs and cover the open reading frames for the viral replicase, the polymerase and the movement and coat proteins (MP, CP). The contigs show identities of 95.3–96.4%, 91.9–93.3% and 89.0–92.5% at the nucleotide level with RNA 1, RNA 2 and RNA 3 of reference sequences, respectively. The analyses of the MP and CP showed significant differences in amino acid sequence compositions compared to those of reference EMoV sequences. These results demonstrate the presence of a so far unknown isolate of EMoV. This is the first report of sequence data of EMoV infecting U. laevis.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 4.0) Attribution 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectUlmus laeviseng
dc.subjectEMoVeng
dc.subjectRNA3 variabilityeng
dc.subjectIllumina high throughput sequencingeng
dc.subject.ddc630 Landwirtschaft und verwandte Bereichenone
dc.subject.ddc570 Biowissenschaften; Biologienone
dc.titleMolecular characterization of the movement and coat proteins of a new Elm mottle virus isolate infecting European white elm (Ulmus laevis Pall.)none
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21466-8
dc.identifier.doihttp://dx.doi.org/10.18452/19934
dc.type.versionpublishedVersionnone
local.edoc.container-titleInternational Journal of Phytopathologynone
local.edoc.pages7none
local.edoc.anmerkungThe final article is available at DOI: 10.33687/phytopath.008.01.2742none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameESci Journals Publishingnone
local.edoc.container-volume8none
local.edoc.container-issue1none
local.edoc.container-firstpage1none
local.edoc.container-lastpage7none
dc.description.versionPeer Reviewednone

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