Show simple item record

2012-10-29Zeitschriftenartikel DOI: 10.3389/fpls.2012.00236
Evidence for a contribution of ALA synthesis to plastid-to-nucleus signaling
dc.contributor.authorCzarnecki, Olaf
dc.contributor.authorGläßer, Christine
dc.contributor.authorChen, Jin-Gui
dc.contributor.authorMayer, Klaus F. X.
dc.contributor.authorGrimm, Bernhard
dc.date.accessioned2019-12-12T11:39:49Z
dc.date.available2019-12-12T11:39:49Z
dc.date.issued2012-10-29none
dc.date.updated2019-10-08T22:37:21Z
dc.identifier.urihttp://edoc.hu-berlin.de/18452/21638
dc.description.abstractThe formation of 5-aminolevulinic acid (ALA) in tetrapyrrole biosynthesis is widely controlled by environmental and metabolic feedback cues that determine the influx into the entire metabolic path. Because of its central role as the rate-limiting step, we hypothesized a potential role of ALA biosynthesis in tetrapyrrole-mediated retrograde signaling and exploited the direct impact of ALA biosynthesis on nuclear gene expression (NGE) by using two different approaches. Firstly, the Arabidopsis gun1, hy1 (gun2), hy2 (gun3), gun4 mutants showing uncoupled NGE from the physiological state of chloroplasts were thoroughly examined for regulatory modifications of ALA synthesis and transcriptional control in the nucleus. We found that reduced ALA-synthesizing capacity is common to analyzed gun mutants. Inhibition of ALA synthesis by gabaculine (GAB) that inactivates glutamate-1-semialdehyde aminotransferase and ALA feeding of wild-type and mutant seedlings corroborate the expression data of gun mutants. Transcript level of photosynthetic marker genes were enhanced in norflurazon (NF)-treated seedlings upon additional GAB treatment, while enhanced ALA amounts diminish these RNA levels in NF-treated wild-type in comparison to the solely NF-treated seedlings. Secondly, the impact of posttranslationally down-regulated ALA synthesis on NGE was investigated by global transcriptome analysis of GAB-treated Arabidopsis seedlings and the gun4-1 mutant, which is also characterized by reduced ALA formation. A common set of significantly modulated genes was identified indicating ALA synthesis as a potential signal emitter. The over-represented gene ontology categories of genes with decreased or increased transcript abundance highlight a few biological processes and cellular functions, which are remarkably affected in response to plastid-localized ALA biosynthesis. These results support the hypothesis that ALA biosynthesis correlates with retrograde signaling-mediated control of NGE.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY 3.0) Attribution 3.0 Unportedger
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectALA synthesiseng
dc.subjectretrograde signalingeng
dc.subjectgabaculineeng
dc.subjectgun mutantseng
dc.subjectmicroarray analysiseng
dc.subject.ddc570 Biowissenschaften; Biologienone
dc.titleEvidence for a contribution of ALA synthesis to plastid-to-nucleus signalingnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/21638-3
dc.identifier.doi10.3389/fpls.2012.00236none
dc.identifier.doihttp://dx.doi.org/10.18452/20922
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in plant sciencenone
local.edoc.pages19none
local.edoc.type-nameZeitschriftenartikel
local.edoc.institutionLebenswissenschaftliche Fakultätnone
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-publisher-nameFrontiers Media S.A.none
local.edoc.container-publisher-placeLausannenone
local.edoc.container-volume3none
dc.description.versionPeer Reviewednone
local.edoc.container-articlenumber236
dc.identifier.eissn1664-462X

Show simple item record