A Fast and Easy Method for Specific Detection of Circular RNA by Rolling-Circle Amplification

Abstract

Let it roll: Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy assay for the detection of natural circRNA, based on rolling‐circle amplification (RCA) and which uses only standard methods such as qPCR and gel electrophoresis.