A Fast and Easy Method for Specific Detection of Circular RNA by Rolling-Circle Amplification
dc.contributor.author | Boss, Marcel | |
dc.contributor.author | Arenz, Christoph | |
dc.date.accessioned | 2020-04-06T12:05:31Z | |
dc.date.available | 2020-04-06T12:05:31Z | |
dc.date.issued | 2019-11-27 | none |
dc.identifier.uri | http://edoc.hu-berlin.de/18452/22077 | |
dc.description.abstract | Let it roll: Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy assay for the detection of natural circRNA, based on rolling‐circle amplification (RCA) and which uses only standard methods such as qPCR and gel electrophoresis. | eng |
dc.language.iso | eng | none |
dc.publisher | Humboldt-Universität zu Berlin | |
dc.rights | (CC BY-NC-ND 4.0) Attribution-NonCommercial-NoDerivatives 4.0 International | ger |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | circRNA | eng |
dc.subject | polymerase chain reaction | eng |
dc.subject | quantitative PCR | eng |
dc.subject | RNA analysis | eng |
dc.subject | rolling-circle amplification | eng |
dc.subject.ddc | 540 Chemie und zugeordnete Wissenschaften | none |
dc.title | A Fast and Easy Method for Specific Detection of Circular RNA by Rolling-Circle Amplification | none |
dc.type | article | |
dc.identifier.urn | urn:nbn:de:kobv:11-110-18452/22077-2 | |
dc.identifier.doi | http://dx.doi.org/10.18452/21336 | |
dc.type.version | publishedVersion | none |
local.edoc.pages | 4 | none |
local.edoc.type-name | Zeitschriftenartikel | |
local.edoc.container-type | periodical | |
local.edoc.container-type-name | Zeitschrift | |
local.edoc.container-year | 2020 | none |
dc.description.version | Peer Reviewed | none |
dc.identifier.eissn | 1439-7633 | |
dcterms.bibliographicCitation.doi | 10.1002/cbic.201900514 | |
dcterms.bibliographicCitation.journaltitle | ChemBioChem | none |
dcterms.bibliographicCitation.volume | 21 | none |
dcterms.bibliographicCitation.issue | 6 | none |
dcterms.bibliographicCitation.originalpublishername | Wiley-VCH | none |
dcterms.bibliographicCitation.originalpublisherplace | Weinheim | none |
dcterms.bibliographicCitation.pagestart | 793 | none |
dcterms.bibliographicCitation.pageend | 796 | none |
bua.department | Mathematisch-Naturwissenschaftliche Fakultät | none |