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2020-04-21Zeitschriftenartikel DOI: 10.18452/21431
A Novel Visible Range FRET Probe for Monitoring Acid Sphingomyelinase Activity in Living Cells
dc.contributor.authorKappe, Christian
dc.contributor.authorMohamed, Zainelabdeen H.
dc.contributor.authorNaser, Eyad
dc.contributor.authorCarpinteiro, Alexander
dc.contributor.authorArenz, Christoph
dc.date.accessioned2020-06-03T14:03:55Z
dc.date.available2020-06-03T14:03:55Z
dc.date.issued2020-04-21none
dc.identifier.urihttp://edoc.hu-berlin.de/18452/22161
dc.description.abstractActivity of acid sphingomyelinase has been implicated in a number of diseases like acute lung injury, sepsis or metastasis of melanoma cells. Here, we present a sphingomyelinase FRET probe based on FAM/BODIPY dyes for real‐time monitoring of acid sphingomyelinase. The probe gives rise to a tremendous increase in fluorescence of the fluorescein FRET donor upon cleavage and we show that this is, to a significant part, due to cleavage‐associated phase transition, suggesting a more systematic consideration of such effects for future probe development. The probe allows for the first time to monitor relative sphingomyelinase activities of intact living cells by flow cytometry.eng
dc.language.isoengnone
dc.publisherHumboldt-Universität zu Berlin
dc.rights(CC BY-NC 4.0) Attribution-NonCommercial 4.0 Internationalger
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectenzyme assayseng
dc.subjectflow cytometryeng
dc.subjectFörster resonance energy transfereng
dc.subjectlive cell assayseng
dc.subjectsphingolipidseng
dc.subject.ddc540 Chemie und zugeordnete Wissenschaftennone
dc.subject.ddc660 Chemische Verfahrenstechnik und verwandte Technologiennone
dc.titleA Novel Visible Range FRET Probe for Monitoring Acid Sphingomyelinase Activity in Living Cellsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:11-110-18452/22161-7
dc.identifier.doihttp://dx.doi.org/10.18452/21431
dc.type.versionpublishedVersionnone
local.edoc.pages4none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
dc.description.versionPeer Reviewednone
dc.identifier.eissn1521-3765
dcterms.bibliographicCitation.doi10.1002/chem.202000133
dcterms.bibliographicCitation.journaltitleChemistry - a European journalnone
dcterms.bibliographicCitation.volume26none
dcterms.bibliographicCitation.issue26none
dcterms.bibliographicCitation.originalpublishernameWiley-VCHnone
dcterms.bibliographicCitation.originalpublisherplaceWeinheimnone
dcterms.bibliographicCitation.pagestart5780none
dcterms.bibliographicCitation.pageend5783none
bua.departmentMathematisch-Naturwissenschaftliche Fakultätnone

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